Analysis of Plant Meiotic Chromosomes by Chromosome Painting

C 2013

Analysis of Plant Meiotic Chromosomes by Chromosome Painting

LYSÁK, Martin a Terezie MANDÁKOVÁ

Základní údaje

Originální název

Analysis of Plant Meiotic Chromosomes by Chromosome Painting

Autoři

LYSÁK, Martin a Terezie MANDÁKOVÁ

Vydání

New York, Plant Meiosis: Methods and Protocols, od s. 13-24, 12 s. Part 1, 2013

Nakladatel

Humana Press

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Kód RIV

RIV/00216224:14740/13:00065831

Organizační jednotka

Středoevropský technologický institut

ISBN

978-1-62703-332-9

DOI

https://doi.org/10.1007/978-1-62703-333-6_2

Klíčová slova anglicky

Chromosome painting; Fluorescence in situ hybridization; BAC FISH; Pachytene chromosomes; DNA labeling

Štítky

ok, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 4. 2014 15:22, Olga Křížová

Anotace

V originále

Chromosome painting (CP) refers to visualization of large chromosome regions, entire chromosome arms, or entire chromosomes via fluorescence in situ hybridization (FISH). For CP in plants, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the target species or from a closely related species (comparative chromosome painting, CCP) are typically applied as painting probes. Extended pachytene chromosomes provide the highest resolution of CP in plants. CP enables identification and tracing of particular chromosome regions and/or entire chromosomes throughout all meiotic stages as well as corresponding chromosome territories in premeiotic interphase nuclei. Meiotic pairing and structural chromosome rearrangements (typically inversions and translocations) can be identified by CP. Here, we describe step-by-step protocols of CP and CCP in plant species including chromosome preparation, BAC DNA labeling, and multicolor FISH.

Návaznosti

GAP501/10/1014, projekt VaV

Název: Evoluce genomu alopolyploidních řeřišnic (Cardamine) rozdílného fylogenetického stáří

Investor: Grantová agentura ČR, Evoluce genomu alopolyploidních řeřišnic (Cardamine) rozdílného fylogenetického stáří

IAA601630902, projekt VaV

Název: Evoluce chromosomů brukvovitých (Brassicaceae) analyzována pomocí komparativního chromosomálního paintingu

Investor: Akademie věd ČR, Evoluce chromosomů brukvovitých (Brassicaceae) analyzována pomocí komparativního chromosomálního paintingu

Citovat

LYSÁK, Martin a Terezie MANDÁKOVÁ. Analysis of Plant Meiotic Chromosomes by Chromosome Painting. In Pawlowski Wojciech P.; Grelon Mathidle; Armstrong Susan. Plant Meiosis: Methods and Protocols. New York: Humana Press, 2013, s. 13-24. Part 1. ISBN 978-1-62703-332-9. Dostupné z: https://doi.org/10.1007/978-1-62703-333-6_2.

@inbook{1164717,
   author = {Lysák, Martin and Mandáková, Terezie},
   address = {New York},
   booktitle = {Plant Meiosis: Methods and Protocols},
   doi = {https://doi.org/10.1007/978-1-62703-333-6_2},
   editor = {Pawlowski Wojciech P.; Grelon Mathidle; Armstrong Susan},
   keywords = {Chromosome painting; Fluorescence in situ hybridization; BAC FISH; Pachytene chromosomes; DNA labeling},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York},
   isbn = {978-1-62703-332-9},
   pages = {13-24},
   publisher = {Humana Press},
   title = {Analysis of Plant Meiotic Chromosomes by Chromosome Painting},
   url = {http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-333-6},
   year = {2013}
}

TY  - CHAP
ID  - 1164717
AU  - Lysák, Martin - Mandáková, Terezie
PY  - 2013
TI  - Analysis of Plant Meiotic Chromosomes by Chromosome Painting
VL  - Part 1
PB  - Humana Press
CY  - New York
SN  - 9781627033329
KW  - Chromosome painting
KW  - Fluorescence in situ hybridization
KW  - BAC FISH
KW  - Pachytene chromosomes
KW  - DNA labeling
UR  - http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-333-6
L2  - http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-333-6
N2  - Chromosome painting (CP) refers to visualization of large chromosome regions, entire chromosome arms, or entire chromosomes via fluorescence in situ hybridization (FISH). For CP in plants, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the target species or from a closely related species (comparative chromosome painting, CCP) are typically applied as painting probes. Extended pachytene chromosomes provide the highest resolution of CP in plants. CP enables identification and tracing of particular chromosome regions and/or entire chromosomes throughout all meiotic stages as well as corresponding chromosome territories in premeiotic interphase nuclei. Meiotic pairing and structural chromosome rearrangements (typically inversions and translocations) can be identified by CP. Here, we describe step-by-step protocols of CP and CCP in plant species including chromosome preparation, BAC DNA labeling, and multicolor FISH.
ER  -

LYSÁK, Martin a Terezie MANDÁKOVÁ. Analysis of Plant Meiotic Chromosomes by Chromosome Painting. In Pawlowski Wojciech P.; Grelon Mathidle; Armstrong Susan. \textit{Plant Meiosis: Methods and Protocols}. New York: Humana Press, 2013, s.~13-24. Part 1. ISBN~978-1-62703-332-9. Dostupné z: https://doi.org/10.1007/978-1-62703-333-6\_{}2.

Přehled o publikaci