A simple Fourier filter for suppression of the missing wedge
ray artefacts in single-axis electron tomographic
reconstructions

J 2014

A simple Fourier filter for suppression of the missing wedge ray artefacts in single-axis electron tomographic reconstructions

KOVÁČIK, Lubomír; Sami KEREÏCHE; Johanna HÖÖG; Pavel JŮDA; Pavel MATULA et al.

Základní údaje

Originální název

A simple Fourier filter for suppression of the missing wedge ray artefacts in single-axis electron tomographic reconstructions

Autoři

KOVÁČIK, Lubomír; Sami KEREÏCHE; Johanna HÖÖG; Pavel JŮDA; Pavel MATULA a Ivan RAŠKA

Vydání

Journal of Structural Biology, Academic Press, 2014, 1047-8477

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.231

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14330/14:00073497

Organizační jednotka

Fakulta informatiky

Klíčová slova anglicky

Electron tomography; Missing wedge; Missing wedge artefacts; Single-axis tilting

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 4. 2015 13:32, doc. RNDr. Pavel Matula, Ph.D.

Anotace

V originále

he limited specimen tilting range that is typically available in electron tomography gives rise to a region in the Fourier space of the reconstructed object where experimental data are unavailable - the missing wedge. Since this region is sharply delimited from the area of available data, the reconstructed signal is typically hampered by convolution with its impulse response, which gives rise to the well-known missing wedge artefacts in 3D reconstructions. Despite the recent progress in the field of reconstruction and regularization techniques, the missing wedge artefacts remain untreated in most current reconstruction workflows in structural biology. Therefore we have designed a simple Fourier angular filter that effectively suppresses the ray artefacts in the single-axis tilting projection acquisition scheme, making single-axis tomographic reconstructions easier to interpret in particular at low signal-to-noise ratio in acquired projections. The proposed filter can be easily incorporated into current electron tomographic reconstruction schemes.

Návaznosti

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

KOVÁČIK, Lubomír; Sami KEREÏCHE; Johanna HÖÖG; Pavel JŮDA; Pavel MATULA a Ivan RAŠKA. A simple Fourier filter for suppression of the missing wedge ray artefacts in single-axis electron tomographic reconstructions. Journal of Structural Biology. Academic Press, 2014, roč. 186, č. 1, s. 141-152. ISSN 1047-8477. Dostupné z: https://doi.org/10.1016/j.jsb.2014.02.004.

@article{1165321,
   author = {Kováčik, Lubomír and Kereïche, Sami and Höög, Johanna and Jůda, Pavel and Matula, Pavel and Raška, Ivan},
   article_number = {1},
   doi = {https://doi.org/10.1016/j.jsb.2014.02.004},
   keywords = {Electron tomography; Missing wedge; Missing wedge artefacts; Single-axis tilting},
   language = {eng},
   issn = {1047-8477},
   journal = {Journal of Structural Biology},
   title = {A simple Fourier filter for suppression of the missing wedge ray artefacts in single-axis electron tomographic reconstructions},
   volume = {186},
   year = {2014}
}

TY  - JOUR
ID  - 1165321
AU  - Kováčik, Lubomír - Kereïche, Sami - Höög, Johanna - Jůda, Pavel - Matula, Pavel - Raška, Ivan
PY  - 2014
TI  - A simple Fourier filter for suppression of the missing wedge ray artefacts in single-axis electron tomographic reconstructions
JF  - Journal of Structural Biology
VL  - 186
IS  - 1
SP  - 141-152
EP  - 141-152
PB  - Academic Press
SN  - 10478477
KW  - Electron tomography
KW  - Missing wedge
KW  - Missing wedge artefacts
KW  - Single-axis tilting
N2  - he limited specimen tilting range that is typically available in electron tomography gives rise to a region in the Fourier space of the reconstructed object where experimental data are unavailable - the missing wedge. Since this region is sharply delimited from the area of available data, the reconstructed signal is typically hampered by convolution with its impulse response, which gives rise to the well-known missing wedge artefacts in 3D reconstructions. Despite the recent progress in the field of reconstruction and regularization techniques, the missing wedge artefacts remain untreated in most current reconstruction workflows in structural biology. Therefore we have designed a simple Fourier angular filter that effectively suppresses the ray artefacts in the single-axis tilting projection acquisition scheme, making single-axis tomographic reconstructions easier to interpret in particular at low signal-to-noise ratio in acquired projections. The proposed filter can be easily incorporated into current electron tomographic reconstruction schemes.
ER  -

KOVÁČIK, Lubomír; Sami KEREÏCHE; Johanna HÖÖG; Pavel JŮDA; Pavel MATULA a Ivan RAŠKA. A simple Fourier filter for suppression of the missing wedge ray artefacts in single-axis electron tomographic reconstructions. \textit{Journal of Structural Biology}. Academic Press, 2014, roč.~186, č.~1, s.~141-152. ISSN~1047-8477. Dostupné z: https://doi.org/10.1016/j.jsb.2014.02.004.

Přehled o publikaci