Oocyst or sporocyst? Another enigma in the development of
cryptosporidia

a 2013

Oocyst or sporocyst? Another enigma in the development of cryptosporidia

MELICHEROVÁ, Janka; Jana ILGOVÁ a Andrea VALIGUROVÁ

Základní údaje

Originální název

Oocyst or sporocyst? Another enigma in the development of cryptosporidia

Autoři

MELICHEROVÁ, Janka; Jana ILGOVÁ a Andrea VALIGUROVÁ

Vydání

43. Jirovcovy Protozoologické dny, 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/13:00066916

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-260-4294-5

Klíčová slova anglicky

oocyst; sporocyst; wall forming bodies;

Změněno: 17. 2. 2014 00:46, Mgr. Janka Melicherová

Anotace

V originále

Developmental stages of the gastric parasite, Cryptosporidium muris (strain TS03), were obtained from stomachs and faeces of experimentally inoculated Mastomys coucha. The development of C. muris oocysts was mapped in detail using a combined microscopic approach, supported by freeze etching. Two types of wall forming bodies (WFB) of different electron density occurred in mature macrogamonts and zygotes. The WFB, located beneath the zygote pellicle, disintegrated into small particles and migrated in the space between pellicular membranes. Additional membranes seemed to develop beneath the pellicle so that four or more membranes could be seen enveloping more advanced zygote stages. Developing oocysts were enveloped by a parasitophorous sac and their wall comprised of three layers. The outermost one, considered to be a true oocyst, was very fragile and this could be the reason that it usually remains unnoticed. In endogenous stages, this layer was usually separated from inner two layers and often almost unnoticeable as it was adjacent to the inner membrane of parasitophorous sac. The middle thin and the innermost thick layers, on which the characteristic suture could be seen, form the wall of a sporocyst. Fully sporulated sporocysts, found either in stomach or faeces, were released from parasitophorous sac and frequently lacked the outermost layer (oocyst). Under scanning electron microscope, they exhibited either smooth or wrinkly surface, presumably depending on the wall thickness. Furthermore, due to a high variability in wall thickness, authors call into question the existence of two independent types of oocysts (thin- and thick-walled) in cryptosporidia. Financial support provided by projects No. P506/10/P372 and MUNI/A/0937/2012.

Návaznosti

GPP506/10/P372, projekt VaV

Název: Srovnávací morfologie a imunohistochemie v hodnocení fylogenetických vztahů mezi zástupci raných linií kmene Apicomplexa

Investor: Grantová agentura ČR, Srovnávací morfologie a imunohistochemie v hodnocení fylogenetických vztahů mezi zástupci raných linií kmene Apicomplexa

Citovat

MELICHEROVÁ, Janka; Jana ILGOVÁ a Andrea VALIGUROVÁ. Oocyst or sporocyst? Another enigma in the development of cryptosporidia. In 43. Jirovcovy Protozoologické dny. 2013. ISBN 978-80-260-4294-5.

@proceedings{1165733,
   author = {Melicherová, Janka and Ilgová, Jana and Valigurová, Andrea},
   booktitle = {43. Jirovcovy Protozoologické dny},
   keywords = {oocyst; sporocyst; wall forming bodies;},
   language = {eng},
   isbn = {978-80-260-4294-5},
   title = {Oocyst or sporocyst? Another enigma in the development of cryptosporidia},
   year = {2013}
}

TY  - CONF
ID  - 1165733
AU  - Melicherová, Janka - Ilgová, Jana - Valigurová, Andrea
PY  - 2013
TI  - Oocyst or sporocyst? Another enigma in the development of cryptosporidia
SN  - 9788026042945
KW  - oocyst
KW  - sporocyst
KW  - wall forming bodies;
N2  - Developmental stages of the gastric parasite, Cryptosporidium muris (strain TS03), were obtained from stomachs and faeces of experimentally inoculated Mastomys coucha. The development of C. muris oocysts was mapped in detail using a combined microscopic approach, supported by freeze etching. Two types of wall forming bodies (WFB) of different electron density occurred in mature macrogamonts and zygotes. The WFB, located beneath the zygote pellicle, disintegrated into small particles and migrated in the space between pellicular membranes. Additional membranes seemed to develop beneath the pellicle so that four or more membranes could be seen enveloping more advanced zygote stages. Developing oocysts were enveloped by a parasitophorous sac and their wall comprised of three layers. The outermost one, considered to be a true oocyst, was very fragile and this could be the reason that it usually remains unnoticed. In endogenous stages, this layer was usually separated from inner two layers and often almost unnoticeable as it was adjacent to the inner membrane of parasitophorous sac. The middle thin and the innermost thick layers, on which the characteristic suture could be seen, form the wall of a sporocyst. Fully sporulated sporocysts, found either in stomach or faeces, were released from parasitophorous sac and frequently lacked the outermost layer (oocyst). Under scanning electron microscope, they exhibited either smooth or wrinkly surface, presumably depending on the wall thickness. Furthermore, due to a high variability in wall thickness, authors call into question the existence of two independent types of oocysts (thin- and thick-walled) in cryptosporidia. Financial support provided by projects No. P506/10/P372 and MUNI/A/0937/2012.
ER  -

MELICHEROVÁ, Janka; Jana ILGOVÁ a Andrea VALIGUROVÁ. Oocyst or sporocyst? Another enigma in the development of cryptosporidia. In \textit{43. Jirovcovy Protozoologické dny}. 2013. ISBN~978-80-260-4294-5.

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