Role of histamine receptors in the effects of histamine on the
production of reactive oxygen species by whole blood phagocytes

J 2014

Role of histamine receptors in the effects of histamine on the production of reactive oxygen species by whole blood phagocytes

VAŠÍČEK, Ondřej; Antonín LOJEK; Viera JANČINOVÁ; Radomír NOSAL; Milan ČÍŽ et al.

Základní údaje

Originální název

Role of histamine receptors in the effects of histamine on the production of reactive oxygen species by whole blood phagocytes

Autoři

VAŠÍČEK, Ondřej; Antonín LOJEK; Viera JANČINOVÁ; Radomír NOSAL a Milan ČÍŽ

Vydání

Life Sciences, Amsterdam, The Netherlands, Elsevier, 2014, 0024-3205

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.702

Označené pro přenos do RIV

DOI

https://doi.org/10.1016/j.lfs.2014.01.082

UT WoS

000334323600009

Klíčová slova anglicky

Histamine; Histamine receptors; H2R; H4R; Reactive oxygen species; Phagocytes

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 2. 2014 11:06, Mgr. Ondřej Vašíček, Ph.D.

Anotace

V originále

Aims The diverse physiological functions of histamine are mediated through distinct histamine receptors. In this study we investigated the role of H2R and H4R in the effects of histamine on the production of reactive oxygen species by phagocytes in whole blood. Main methods Changes in reactive oxygen species (ROS) production by whole blood phagocytes after treatment with histamine, H4R agonists (4-methylhistamine, VUF8430), H2R agonist (dimaprit) and their combinations with H4R antagonist (JNJ10191584) and H2R antagonist (ranitidine) were determined using the chemiluminescence (CL) assay. To exclude the direct scavenging effects of the studied compounds on the CL response, the antioxidant properties of all compounds were measured using several methods (TRAP, ORAC, and luminol HRP H2O2 based CL). Key findings Histamine, 4methylhistamine, VUF8430 and dimaprit inhibited the spontaneous and OZP activated whole blood CL in a dose dependent manner. On the other hand, only VUF8430 was able to inhibit PMA activated whole blood CL. Ranitidine, but not JNJ10191584, completely reduced the effects of histamine, 4methylhistamine and dimaprit. The direct scavenging ability of tested compounds was negligible. Significance Our results demonstrate that the inhibitory effects of histamine on ROS production in whole blood phagocytes were caused by H2R. Our results also suggest that H4R agonists in concentrations higher than 1uM may also influence ROS production via binding to H2R.

Citovat

VAŠÍČEK, Ondřej; Antonín LOJEK; Viera JANČINOVÁ; Radomír NOSAL a Milan ČÍŽ. Role of histamine receptors in the effects of histamine on the production of reactive oxygen species by whole blood phagocytes. Life Sciences. Amsterdam, The Netherlands,: Elsevier, 2014. ISSN 0024-3205. Dostupné z: https://doi.org/10.1016/j.lfs.2014.01.082.

@article{1166955,
   author = {Vašíček, Ondřej and Lojek, Antonín and Jančinová, Viera and Nosal, Radomír and Číž, Milan},
   article_location = {Amsterdam, The Netherlands,},
   doi = {https://doi.org/10.1016/j.lfs.2014.01.082},
   keywords = {Histamine; Histamine receptors; H2R; H4R; Reactive oxygen species; Phagocytes},
   language = {eng},
   issn = {0024-3205},
   journal = {Life Sciences},
   title = {Role of histamine receptors in the effects of histamine on the production of reactive oxygen species by whole blood phagocytes},
   url = {http://www.sciencedirect.com/science/article/pii/S0024320514002203},
   year = {2014}
}

TY  - JOUR
ID  - 1166955
AU  - Vašíček, Ondřej - Lojek, Antonín - Jančinová, Viera - Nosal, Radomír - Číž, Milan
PY  - 2014
TI  - Role of histamine receptors in the effects of histamine on the production of reactive oxygen species by whole blood phagocytes
JF  - Life Sciences
PB  - Elsevier
SN  - 00243205
KW  - Histamine
KW  - Histamine receptors
KW  - H2R
KW  - H4R
KW  - Reactive oxygen species
KW  - Phagocytes
UR  - http://www.sciencedirect.com/science/article/pii/S0024320514002203
N2  - Aims The diverse physiological functions of histamine are mediated through distinct histamine receptors. In this study we investigated the role of H2R and H4R in the effects of histamine on the production of reactive oxygen species by phagocytes in whole blood. Main methods Changes in reactive oxygen species (ROS) production by whole blood phagocytes after treatment with histamine, H4R agonists (4-methylhistamine, VUF8430), H2R agonist (dimaprit) and their combinations with H4R antagonist (JNJ10191584) and H2R antagonist (ranitidine) were determined using the chemiluminescence (CL) assay. To exclude the direct scavenging effects of the studied compounds on the CL response, the antioxidant properties of all compounds were measured using several methods (TRAP, ORAC, and luminol HRP H2O2 based CL). Key findings Histamine, 4methylhistamine, VUF8430 and dimaprit inhibited the spontaneous and OZP activated whole blood CL in a dose dependent manner. On the other hand, only VUF8430 was able to inhibit PMA activated whole blood CL. Ranitidine, but not JNJ10191584, completely reduced the effects of histamine, 4methylhistamine and dimaprit. The direct scavenging ability of tested compounds was negligible. Significance Our results demonstrate that the inhibitory effects of histamine on ROS production in whole blood phagocytes were caused by H2R. Our results also suggest that H4R agonists in concentrations higher than 1uM may also influence ROS production via binding to H2R.
ER  -

VAŠÍČEK, Ondřej; Antonín LOJEK; Viera JANČINOVÁ; Radomír NOSAL a Milan ČÍŽ. Role of histamine receptors in the effects of histamine on the production of reactive oxygen species by whole blood phagocytes. \textit{Life Sciences}. Amsterdam, The Netherlands,: Elsevier, 2014. ISSN~0024-3205. Dostupné z: https://doi.org/10.1016/j.lfs.2014.01.082.

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