Femtogram Electrochemical Sensing of Prion Proteins Using
Quantum Dots

J 2013

Femtogram Electrochemical Sensing of Prion Proteins Using Quantum Dots

ŠOBROVÁ, Pavlína, Markéta RYVOLOVÁ, Vladimír PEKAŘÍK, Jaromír HUBÁLEK, Vojtěch ADAM et. al.

Basic information

Original name

Femtogram Electrochemical Sensing of Prion Proteins Using Quantum Dots

Authors

ŠOBROVÁ, Pavlína (203 Czech Republic), Markéta RYVOLOVÁ (203 Czech Republic), Vladimír PEKAŘÍK (203 Czech Republic, guarantor, belonging to the institution), Jaromír HUBÁLEK (203 Czech Republic), Vojtěch ADAM (203 Czech Republic) and René KIZEK (203 Czech Republic)

Edition

International Journal of Electrochemical Science, Belgrade, ESG, 2013, 1452-3981

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Serbia

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.956

RIV identification code

RIV/00216224:14740/13:00072405

Organization unit

Central European Institute of Technology

UT WoS

000326961400006

Keywords in English

Quantum Dot; Prion Proteins; In Vivo Imaging; Electrochemistry; Differential Pulse Voltammetry

Abstract

V originále

The prion protein (PrP) is involved in neurodegeneration via its conversion from the normal cellular form, PrPC, to the infectious form, PrPSc, which is the causative agent of the transmissible spongiform encephalopathies (TSEs) including Creutzfeldt-Jakob disease (CJD). In spite of great effort in this field, diagnostics of prion protein caused diseases represents a sort of challenge. In this study, we aimed our attention on studying of prion protein interaction with CdTe quantum dots (QDs) by voltammetry as a new and extremely sensitive tool for sensing of these proteins. Primarily, we characterized fluorescent and electrochemical properties of QDs. Further, electrochemical study of their interactions was carried out to find the most suitable conditions for sensitive detection of prion proteins. Detection limit (3 S/N) was estimated as 1 fg in 5 mu l. This makes labeling of proteins with QDs of great importance due to easy applicability and possibility to use in miniaturized devices, which can be used in situ. Based on our results it can be concluded that QDs-prion protein complex is stable and can be quantified in extremely low amounts. This should open new possibilities how to determine the presence of these proteins on surgical equipment and other types of materials, which could be contagious.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

ŠOBROVÁ, Pavlína, Markéta RYVOLOVÁ, Vladimír PEKAŘÍK, Jaromír HUBÁLEK, Vojtěch ADAM and René KIZEK. Femtogram Electrochemical Sensing of Prion Proteins Using Quantum Dots. International Journal of Electrochemical Science. Belgrade: ESG, 2013, vol. 8, No 12, p. 12466-12475. ISSN 1452-3981.

@article{1167373,
   author = {Šobrová, Pavlína and Ryvolová, Markéta and Pekařík, Vladimír and Hubálek, Jaromír and Adam, Vojtěch and Kizek, René},
   article_location = {Belgrade},
   article_number = {12},
   keywords = {Quantum Dot; Prion Proteins; In Vivo Imaging; Electrochemistry; Differential Pulse Voltammetry},
   language = {eng},
   issn = {1452-3981},
   journal = {International Journal of Electrochemical Science},
   title = {Femtogram Electrochemical Sensing of Prion Proteins Using Quantum Dots},
   url = {http://www.electrochemsci.org/papers/vol8/81212466.pdf},
   volume = {8},
   year = {2013}
}

TY  - JOUR
ID  - 1167373
AU  - Šobrová, Pavlína - Ryvolová, Markéta - Pekařík, Vladimír - Hubálek, Jaromír - Adam, Vojtěch - Kizek, René
PY  - 2013
TI  - Femtogram Electrochemical Sensing of Prion Proteins Using Quantum Dots
JF  - International Journal of Electrochemical Science
VL  - 8
IS  - 12
SP  - 12466-12475
EP  - 12466-12475
PB  - ESG
SN  - 14523981
KW  - Quantum Dot
KW  - Prion Proteins
KW  - In Vivo Imaging
KW  - Electrochemistry
KW  - Differential Pulse Voltammetry
UR  - http://www.electrochemsci.org/papers/vol8/81212466.pdf
L2  - http://www.electrochemsci.org/papers/vol8/81212466.pdf
N2  - The prion protein (PrP) is involved in neurodegeneration via its conversion from the normal cellular form, PrPC, to the infectious form, PrPSc, which is the causative agent of the transmissible spongiform encephalopathies (TSEs) including Creutzfeldt-Jakob disease (CJD). In spite of great effort in this field, diagnostics of prion protein caused diseases represents a sort of challenge. In this study, we aimed our attention on studying of prion protein interaction with CdTe quantum dots (QDs) by voltammetry as a new and extremely sensitive tool for sensing of these proteins. Primarily, we characterized fluorescent and electrochemical properties of QDs. Further, electrochemical study of their interactions was carried out to find the most suitable conditions for sensitive detection of prion proteins. Detection limit (3 S/N) was estimated as 1 fg in 5 mu l. This makes labeling of proteins with QDs of great importance due to easy applicability and possibility to use in miniaturized devices, which can be used in situ. Based on our results it can be concluded that QDs-prion protein complex is stable and can be quantified in extremely low amounts. This should open new possibilities how to determine the presence of these proteins on surgical equipment and other types of materials, which could be contagious.
ER  -

ŠOBROVÁ, Pavlína, Markéta RYVOLOVÁ, Vladimír PEKAŘÍK, Jaromír HUBÁLEK, Vojtěch ADAM and René KIZEK. Femtogram Electrochemical Sensing of Prion Proteins Using Quantum Dots. \textit{International Journal of Electrochemical Science}. Belgrade: ESG, 2013, vol.~8, No~12, p.~12466-12475. ISSN~1452-3981.

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