Study of the Receiver and Receiver-like Domain Relationship in
Cytokinin Receptor of Arabidopsis thaliana

a 2013

Study of the Receiver and Receiver-like Domain Relationship in Cytokinin Receptor of Arabidopsis thaliana

HRDINOVÁ, Vendula, Blanka PEKÁROVÁ, Tomáš KLUMPLER, Lubomír JANDA, Jan HEJÁTKO et. al.

Základní údaje

Originální název

Study of the Receiver and Receiver-like Domain Relationship in Cytokinin Receptor of Arabidopsis thaliana

Autoři

HRDINOVÁ, Vendula, Blanka PEKÁROVÁ, Tomáš KLUMPLER, Lubomír JANDA a Jan HEJÁTKO

Vydání

ITAT 2013: Information Technologies—Applications and Theory, 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-1-4909-5208-6

Klíčová slova anglicky

cytokinin domain protein bioinformatics

Změněno: 10. 3. 2014 10:35, Ing. Vendula Hrdinová

Anotace

V originále

Cytokinin (CK) signaling is one of the pathways mediated by multistep phosphorelay (MSP) in plants. In the MSP of Arabidopsis thaliana, Arabidopsis histidine kinases (AHKs) act as receptors initiating the pathway and interact with Arabidopsis histidine-containing proteins (AHPs), thus transferring the signal farther in the cascade. For the signal transduction through the cascade, the domain composition of the MSP elements is crucial. Recently it was reported that the receiver domain of one of the Arabidopsis sensor histidine kinases CKI1 is sufficient and necessary for the specific interaction of CKI1 with AHPs. Here we performed an extensive study of the individual domains of CK receptor AHK4 in mediating interactions with AHPs. Thorough bioinformatic analysis of the AHK4 aa sequence using similarity search was carried out to refine the precise borders of AHK4 individual domains. SMART and PROSITE databases for primary and secondary structure analysis were used to identify similarities within receiver and receiver-like domains of other AHKs and the results were manually refined. Moreover, we modeled individual structures of the receiver and receiver-like domain of AHK4 (AHK4 RD and AHK4 RLD , respectively) employing their homology with solved structure of bacterial response regulator receiver domain CheY in order to see possible differences in the structure and to predict potential interaction interfaces. The structural predictions were used to design recombinant DNA constructs for protein-protein interaction experiments. Intriguingly, by yeast two-hybrid analysis we revealed that not only AHK4 RD is substantial for the interaction with AHPs but we found out that the interaction can be suppressed by AHK4 RLD . We propose that AHK4 RLD has a (negative) regulatory function in the process of molecular recognition of the downstream signaling partners and we experimentally demonstrated that this function can be provided by the heterodimerization of AHK4 RD with AHK4RLD, either intra- or intermolecularly. The structural prediction-based model of this regulatory role will be presented.

Citovat

HRDINOVÁ, Vendula, Blanka PEKÁROVÁ, Tomáš KLUMPLER, Lubomír JANDA a Jan HEJÁTKO. Study of the Receiver and Receiver-like Domain Relationship in Cytokinin Receptor of Arabidopsis thaliana. In ITAT 2013: Information Technologies—Applications and Theory. 2013. ISBN 978-1-4909-5208-6.

@proceedings{1169077,
   author = {Hrdinová, Vendula and Pekárová, Blanka and Klumpler, Tomáš and Janda, Lubomír and Hejátko, Jan},
   booktitle = {ITAT 2013: Information Technologies—Applications and Theory},
   keywords = {cytokinin domain protein bioinformatics},
   language = {eng},
   isbn = {978-1-4909-5208-6},
   title = {Study of the Receiver and Receiver-like Domain Relationship in Cytokinin Receptor of Arabidopsis thaliana},
   year = {2013}
}

TY  - CONF
ID  - 1169077
AU  - Hrdinová, Vendula - Pekárová, Blanka - Klumpler, Tomáš - Janda, Lubomír - Hejátko, Jan
PY  - 2013
TI  - Study of the Receiver and Receiver-like Domain Relationship in Cytokinin Receptor of Arabidopsis thaliana
SN  - 9781490952086
KW  - cytokinin domain protein bioinformatics
N2  - Cytokinin (CK) signaling is one of the pathways mediated by multistep phosphorelay (MSP) in plants. In the MSP of Arabidopsis thaliana, Arabidopsis histidine kinases (AHKs) act as receptors initiating the pathway and interact with Arabidopsis histidine-containing proteins (AHPs), thus transferring the signal farther in the cascade. For the signal transduction through the cascade, the domain composition of the MSP elements is crucial. Recently it was reported that the receiver domain of one of the Arabidopsis sensor histidine kinases CKI1 is sufficient and necessary for the specific interaction of CKI1 with AHPs. Here we performed an extensive study of the individual domains of CK receptor AHK4 in mediating interactions with AHPs. Thorough bioinformatic analysis of the AHK4 aa sequence using similarity search was carried out to refine the precise borders of AHK4 individual domains. SMART and PROSITE databases for primary and secondary structure analysis were used to identify similarities within receiver and receiver-like domains of other AHKs and the results were manually refined. Moreover, we modeled individual structures of the receiver and receiver-like domain of AHK4 (AHK4 RD and AHK4 RLD , respectively) employing their homology with solved structure of bacterial response regulator receiver domain CheY in order to see possible differences in the structure and to predict potential interaction interfaces. The structural predictions were used to design recombinant DNA constructs for protein-protein interaction experiments. Intriguingly, by yeast two-hybrid analysis we revealed that not only AHK4 RD is substantial for the interaction with AHPs but we found out that the interaction can be suppressed by AHK4 RLD . We propose that AHK4 RLD has a (negative) regulatory function in the process of molecular recognition of the downstream signaling partners and we experimentally demonstrated that this function can be provided by the heterodimerization of AHK4 RD with AHK4RLD, either intra- or intermolecularly. The structural prediction-based model of this regulatory role will be presented.
ER  -

HRDINOVÁ, Vendula, Blanka PEKÁROVÁ, Tomáš KLUMPLER, Lubomír JANDA a Jan HEJÁTKO. Study of the Receiver and Receiver-like Domain Relationship in Cytokinin Receptor of Arabidopsis thaliana. In \textit{ITAT 2013: Information Technologies—Applications and Theory}. 2013. ISBN~978-1-4909-5208-6.

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