POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF
CARE DIGNOSTICS

D 2012

POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS

KUBÁŇ, Petr, Eeva-Gerda KOBRIN a Kaljurand MIHKEL

Základní údaje

Originální název

POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS

Autoři

KUBÁŇ, Petr (203 Česká republika, garant, domácí), Eeva-Gerda KOBRIN (233 Estonsko) a Kaljurand MIHKEL (233 Estonsko)

Vydání

Brno, CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS, od s. 128-133, 6 s. 2012

Nakladatel

Ústav analytické chemie AV ČR, v. v. i

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Kód RIV

RIV/00216224:14740/12:00081987

Organizační jednotka

Středoevropský technologický institut

ISBN

978-80-904959-1-3

UT WoS

000329635000041

Klíčová slova česky

kondenzát vydechovaného vzduch; kapilární elektroforéza; kapilární elektroforéza; point of care diagnostika

Klíčová slova anglicky

Exhaled breath condensate; capillary electrophoresis; point of care diagnostics

Štítky

kontrola MP, rivok

Příznaky

Mezinárodní význam

Změněno: 25. 9. 2015 10:21, Martina Prášilová

Anotace

V originále

Exhaled breath condensate has been analyzed for its ionic content by capillary electrophoresis with capacitively coupled contactless conductometric detection. Two devices for exhaled breath condensate collection were compared. These include a tube-in-tube cooled sampler and a simple zip-lock bag. The devices allow collection of small volumes (100-200 mu L) of exhaled breath condensate in less than 2 min. A method for quick (less than 3 min) simultaneous determination of inorganic cations, inorganic anions and organic anions from the samples using dual-opposite end injection principle with a short fused silica capillary (35 cm, 50 mu m i.d.) was optimized with final background electrolyte composition of 20 mM 2-(N-morpholino) ethanesulfonic acid, 20 mM L-histidine, 30 mu M cetyltrimethylammonium bromide and 2 mM 18-crown-6 was used. It has been shown that changes of nitrite could be observed in acute inflammation of upper airways and in person with diagnosed mild chronic obstructive pulmonary disease, while the changes of other ions could also be observed.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

KUBÁŇ, Petr, Eeva-Gerda KOBRIN a Kaljurand MIHKEL. POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS. In Foret, F; Krenkova, J; Guttman, A; Kleparnik, K; Bocek, P. CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS. Brno: Ústav analytické chemie AV ČR, v. v. i, 2012, s. 128-133. ISBN 978-80-904959-1-3.

@inproceedings{1169981,
   author = {Kubáň, Petr and Kobrin, EevaandGerda and Mihkel, Kaljurand},
   address = {Brno},
   booktitle = {CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS},
   editor = {Foret, F; Krenkova, J; Guttman, A; Kleparnik, K; Bocek, P},
   keywords = {Exhaled breath condensate; capillary electrophoresis; point of care diagnostics},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-904959-1-3},
   pages = {128-133},
   publisher = {Ústav analytické chemie AV ČR, v. v. i},
   title = {POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS},
   year = {2012}
}

TY  - JOUR
ID  - 1169981
AU  - Kubáň, Petr - Kobrin, Eeva-Gerda - Mihkel, Kaljurand
PY  - 2012
TI  - POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS
PB  - Ústav analytické chemie AV ČR, v. v. i
CY  - Brno
SN  - 9788090495913
KW  - Exhaled breath condensate
KW  - capillary electrophoresis
KW  - point of care diagnostics
N2  - Exhaled breath condensate has been analyzed for its ionic content by capillary electrophoresis with capacitively coupled contactless conductometric detection. Two devices for exhaled breath condensate collection were compared. These include a tube-in-tube cooled sampler and a simple zip-lock bag. The devices allow collection of small volumes (100-200 mu L) of exhaled breath condensate in less than 2 min. A method for quick (less than 3 min) simultaneous determination of inorganic cations, inorganic anions and organic anions from the samples using dual-opposite end injection principle with a short fused silica capillary (35 cm, 50 mu m i.d.) was optimized with final background electrolyte composition of 20 mM 2-(N-morpholino) ethanesulfonic acid, 20 mM L-histidine, 30 mu M cetyltrimethylammonium bromide and 2 mM 18-crown-6 was used. It has been shown that changes of nitrite could be observed in acute inflammation of upper airways and in person with diagnosed mild chronic obstructive pulmonary disease, while the changes of other ions could also be observed.
ER  -

KUBÁŇ, Petr, Eeva-Gerda KOBRIN a Kaljurand MIHKEL. POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS. In Foret, F; Krenkova, J; Guttman, A; Kleparnik, K; Bocek, P. \textit{CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS}. Brno: Ústav analytické chemie AV ČR, v. v. i, 2012, s.~128-133. ISBN~978-80-904959-1-3.

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