| DADOVÁ, Jitka, Pavlína VIDLÁKOVÁ, Radek POHL, Luděk HAVRAN, Miroslav FOJTA a Michal HOCEK. Aqueous Heck Cross-Coupling Preparation of Acrylate-Modified Nucleotides and Nucleoside Triphosphates for Polymerase Synthesis of Acrylate-Labeled DNA. The journal of organic chemistry. Washington: American Chemical Society, 2013, roč. 78, č. 19, s. 9627-9637. ISSN 0022-3263. Dostupné z: https://dx.doi.org/10.1021/jo4011574. |
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@article{1170041,
author = {Dadová, Jitka and Vidláková, Pavlína and Pohl, Radek and Havran, Luděk and Fojta, Miroslav and Hocek, Michal},
article_location = {Washington},
article_number = {19},
doi = {http://dx.doi.org/10.1021/jo4011574},
keywords = {DNA polymerase; DNA labelling; nucleoside triphosphates},
language = {eng},
issn = {0022-3263},
journal = {The journal of organic chemistry},
title = {Aqueous Heck Cross-Coupling Preparation of Acrylate-Modified Nucleotides and Nucleoside Triphosphates for Polymerase Synthesis of Acrylate-Labeled DNA},
volume = {78},
year = {2013}
}
TY - JOUR
ID - 1170041
AU - Dadová, Jitka - Vidláková, Pavlína - Pohl, Radek - Havran, Luděk - Fojta, Miroslav - Hocek, Michal
PY - 2013
TI - Aqueous Heck Cross-Coupling Preparation of Acrylate-Modified Nucleotides and Nucleoside Triphosphates for Polymerase Synthesis of Acrylate-Labeled DNA
JF - The journal of organic chemistry
VL - 78
IS - 19
SP - 9627-9637
EP - 9627-9637
PB - American Chemical Society
SN - 00223263
KW - DNA polymerase
KW - DNA labelling
KW - nucleoside triphosphates
N2 - Aqueous-phase Heck coupling methodology was developed for direct attachment of butyl acrylate to 5-iodoracil, 5-iodocytosine, 7-iodo-7-deazaadenine, and 7-iodo-7-deazaguanine 2'-deoxyribonucleoside 5'-O-monophosphates (dNMPs) and 5'-O-triphosphates (dNTPs) and compared with the classical approach of phosphorylation of the corresponding modified nucleosides. The acrylate-modified dN(BA)TPs (N = A, C, and U) were good substrates for DNA polymerases and were used for enzymatic synthesis of acrylate-modified DNA by primer extension, whereas dG(BA)TP was an inhibitor of polymerases.
ER -
DADOVÁ, Jitka, Pavlína VIDLÁKOVÁ, Radek POHL, Luděk HAVRAN, Miroslav FOJTA a Michal HOCEK. Aqueous Heck Cross-Coupling Preparation of Acrylate-Modified Nucleotides and Nucleoside Triphosphates for Polymerase Synthesis of Acrylate-Labeled DNA. \textit{The journal of organic chemistry}. Washington: American Chemical Society, 2013, roč.~78, č.~19, s.~9627-9637. ISSN~0022-3263. Dostupné z: https://dx.doi.org/10.1021/jo4011574.
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