Polymerase synthesis of oligonucleotides containing a single
chemically modified nucleobase for site-specific redox
labelling

J 2013

Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling

MÉNOVÁ, Petra; Hana CAHOVÁ; Medard PLUCNARA; Luděk HAVRAN; Miroslav FOJTA et al.

Základní údaje

Originální název

Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling

Autoři

MÉNOVÁ, Petra; Hana CAHOVÁ; Medard PLUCNARA; Luděk HAVRAN; Miroslav FOJTA a Michal HOCEK

Vydání

Chemical communications, Cambridge, Royal Society of Chemistry, 2013, 1359-7345

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.718

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/13:00067006

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1039/C3CC41438H

UT WoS

000318028500020

Klíčová slova anglicky

DNA polymerase; DNA labelling; oligonucleotides

Štítky

DNA labelling, DNA polymerase, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 3. 2014 13:28, Olga Křížová

Anotace

V originále

Enzymatic construction of single-nucleobase redox-labelled oligonucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magnetoseparation and the second PEX with a full-length template.

Návaznosti

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

MÉNOVÁ, Petra; Hana CAHOVÁ; Medard PLUCNARA; Luděk HAVRAN; Miroslav FOJTA a Michal HOCEK. Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling. Chemical communications. Cambridge: Royal Society of Chemistry, 2013, roč. 49, č. 41, s. 4652-4654. ISSN 1359-7345. Dostupné z: https://doi.org/10.1039/C3CC41438H.

@article{1170046,
   author = {Ménová, Petra and Cahová, Hana and Plucnara, Medard and Havran, Luděk and Fojta, Miroslav and Hocek, Michal},
   article_location = {Cambridge},
   article_number = {41},
   doi = {https://doi.org/10.1039/C3CC41438H},
   keywords = {DNA polymerase; DNA labelling; oligonucleotides},
   language = {eng},
   issn = {1359-7345},
   journal = {Chemical communications},
   title = {Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling},
   url = {http://www.rsc.org/chemcomm},
   volume = {49},
   year = {2013}
}

TY  - JOUR
ID  - 1170046
AU  - Ménová, Petra - Cahová, Hana - Plucnara, Medard - Havran, Luděk - Fojta, Miroslav - Hocek, Michal
PY  - 2013
TI  - Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling
JF  - Chemical communications
VL  - 49
IS  - 41
SP  - 4652-4654
EP  - 4652-4654
PB  - Royal Society of Chemistry
SN  - 13597345
KW  - DNA polymerase
KW  - DNA labelling
KW  - oligonucleotides
UR  - http://www.rsc.org/chemcomm
L2  - http://www.rsc.org/chemcomm
N2  - Enzymatic construction of single-nucleobase redox-labelled oligonucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magnetoseparation and the second PEX with a full-length template.
ER  -

MÉNOVÁ, Petra; Hana CAHOVÁ; Medard PLUCNARA; Luděk HAVRAN; Miroslav FOJTA a Michal HOCEK. Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling. \textit{Chemical communications}. Cambridge: Royal Society of Chemistry, 2013, roč.~49, č.~41, s.~4652-4654. ISSN~1359-7345. Dostupné z: https://doi.org/10.1039/C3CC41438H.

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