Lack of interaction of endocannabinoids and 5-HT3
neurotransmission in associative fear circuits of the amygdala:
Evidence from electrophysiological and behavioural experiments

J 2013

Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments

HOFELMANN, D.; B. DI BENEDETTO; S. C. AZAD; Vincenzo MICALE; C.T. WOTJAK et. al.

Základní údaje

Originální název

Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments

Autoři

HOFELMANN, D. (276 Německo); B. DI BENEDETTO (276 Německo); S. C. AZAD (276 Německo); Vincenzo MICALE (380 Itálie, garant, domácí); C.T. WOTJAK (276 Německo) a G. RAMMES (276 Německo)

Vydání

Brain Research, Amsterdam, Elsevier, 2013, 0006-8993

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.828

Kód RIV

RIV/00216224:14740/13:00072700

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1016/j.brainres.2013.06.011

UT WoS

000324225400005

Klíčová slova anglicky

CB1; 5-HT3; Amygdala; Fear conditioning; Slice; IPSC

Štítky

ok, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 3. 2014 11:26, Olga Křížová

Anotace

V originále

Both the serotonergic and the endocannabinoid system play a major role in mediating fear and anxiety. In the basolateral amygdala (BLA) it has been shown that the cannabinoid receptor 1 (CB1) is highly co-expressed with 5-HT3 receptors on GABAergic intemeurons suggesting that 5-HT3 receptor activity modulates CB1-mediated effects on inhibitory synaptic transmission. In the present study, we investigated the possible interactions of CB1 and 5-HT3-mediated neuronal processes in the BLA using electrophysiological and behavioural approaches. Whole-cell patch-clamp recordings were performed in coronal brain slices of mice. Electric stimuli were delivered to the lateral amygdala to evoke GABA(A) receptor-mediated inhibitory postsynaptic currents (GABA(A)-eIPSCs) in the BLA. The induction of LTDi, a CB1-mediated depression of inhibitory synaptic transmission, was neither affected by the 5-HT3 antagonists ondansetron (OND; 20 mu M) and tropisetron (Trop; 50 nM) nor by the 5-HT3 agonists SR57227A (10 mu M). In auditory fear conditioning tests, mice treated with SR57227A (3.0 mg/kg i.p.) showed sustained freezing, whereas treatment with Trop (1.0 mg/kg i.p.) decreased the expression of conditioned fear. These effects were overruled by the CB1 antagonist rimonabant (RIM; 3.0 mg/kg), which caused increased freezing with or without co-treatment with Trop. In summary, these experiments do not support a functional interaction between CB1 and 5-HT3 receptors at the level of GABA neurotransmission in the BLA nor in terms of fear regulation. (C) 2013 Elsevier B.V. All rights reserved.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

HOFELMANN, D.; B. DI BENEDETTO; S. C. AZAD; Vincenzo MICALE; C.T. WOTJAK a G. RAMMES. Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments. Brain Research. Amsterdam: Elsevier, 2013, roč. 1527, Aug, s. 47-56. ISSN 0006-8993. Dostupné z: https://doi.org/10.1016/j.brainres.2013.06.011.

@article{1170127,
   author = {Hofelmann, D. and di Benedetto, B. and Azad, S. C. and Micale, Vincenzo and Wotjak, C.T. and Rammes, G.},
   article_location = {Amsterdam},
   article_number = {Aug},
   doi = {https://doi.org/10.1016/j.brainres.2013.06.011},
   keywords = {CB1; 5-HT3; Amygdala; Fear conditioning; Slice; IPSC},
   language = {eng},
   issn = {0006-8993},
   journal = {Brain Research},
   title = {Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments},
   url = {http://www.sciencedirect.com/science/article/pii/S0006899313008627#},
   volume = {1527},
   year = {2013}
}

TY  - JOUR
ID  - 1170127
AU  - Hofelmann, D. - di Benedetto, B. - Azad, S. C. - Micale, Vincenzo - Wotjak, C.T. - Rammes, G.
PY  - 2013
TI  - Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments
JF  - Brain Research
VL  - 1527
IS  - Aug
SP  - 47-56
EP  - 47-56
PB  - Elsevier
SN  - 00068993
KW  - CB1
KW  - 5-HT3
KW  - Amygdala
KW  - Fear conditioning
KW  - Slice
KW  - IPSC
UR  - http://www.sciencedirect.com/science/article/pii/S0006899313008627#
L2  - http://www.sciencedirect.com/science/article/pii/S0006899313008627#
N2  - Both the serotonergic and the endocannabinoid system play a major role in mediating fear and anxiety. In the basolateral amygdala (BLA) it has been shown that the cannabinoid receptor 1 (CB1) is highly co-expressed with 5-HT3 receptors on GABAergic intemeurons suggesting that 5-HT3 receptor activity modulates CB1-mediated effects on inhibitory synaptic transmission. In the present study, we investigated the possible interactions of CB1 and 5-HT3-mediated neuronal processes in the BLA using electrophysiological and behavioural approaches. Whole-cell patch-clamp recordings were performed in coronal brain slices of mice. Electric stimuli were delivered to the lateral amygdala to evoke GABA(A) receptor-mediated inhibitory postsynaptic currents (GABA(A)-eIPSCs) in the BLA. The induction of LTDi, a CB1-mediated depression of inhibitory synaptic transmission, was neither affected by the 5-HT3 antagonists ondansetron (OND; 20 mu M) and tropisetron (Trop; 50 nM) nor by the 5-HT3 agonists SR57227A (10 mu M). In auditory fear conditioning tests, mice treated with SR57227A (3.0 mg/kg i.p.) showed sustained freezing, whereas treatment with Trop (1.0 mg/kg i.p.) decreased the expression of conditioned fear. These effects were overruled by the CB1 antagonist rimonabant (RIM; 3.0 mg/kg), which caused increased freezing with or without co-treatment with Trop. In summary, these experiments do not support a functional interaction between CB1 and 5-HT3 receptors at the level of GABA neurotransmission in the BLA nor in terms of fear regulation. (C) 2013 Elsevier B.V. All rights reserved.
ER  -

HOFELMANN, D.; B. DI BENEDETTO; S. C. AZAD; Vincenzo MICALE; C.T. WOTJAK a G. RAMMES. Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments. \textit{Brain Research}. Amsterdam: Elsevier, 2013, roč.~1527, Aug, s.~47-56. ISSN~0006-8993. Dostupné z: https://doi.org/10.1016/j.brainres.2013.06.011.

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