Light-Switchable Polymer from Cationic to Zwitterionic Form:
Synthesis, Characterization, and Interactions with DNA and
Bacterial Cells

J 2013

Light-Switchable Polymer from Cationic to Zwitterionic Form: Synthesis, Characterization, and Interactions with DNA and Bacterial Cells

SOBOLČIAK, Patrik, Mário ŠPÍREK, Jaroslav KATRLÍK, Peter GEMEINER, Igor LACÍK et. al.

Základní údaje

Originální název

Light-Switchable Polymer from Cationic to Zwitterionic Form: Synthesis, Characterization, and Interactions with DNA and Bacterial Cells

Autoři

SOBOLČIAK, Patrik (703 Slovensko), Mário ŠPÍREK (703 Slovensko, garant, domácí), Jaroslav KATRLÍK (703 Slovensko), Peter GEMEINER (703 Slovensko), Igor LACÍK (703 Slovensko) a Peter KASÁK (703 Slovensko)

Vydání

MACROMOLECULAR RAPID COMMUNICATIONS, 2013, 1022-1336

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10404 Polymer science

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.608

Kód RIV

RIV/00216224:14110/13:00072702

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1002/marc.201200823

UT WoS

000318029800003

Klíčová slova anglicky

antibacterial; carboxybetaine ester; dsDNA polyplex; photochemistry; water-soluble polymers

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 3. 2014 13:57, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

A novel cationic polymer poly(N,N-dimethyl-N-[3-(methacroylamino) propyl]-N-[2-[(2-nitrophenyl)methoxy]-2-oxo-ethyl]ammonium chloride) is synthesized by free-radical polymerization of N-[3-(dimethylamino)propyl] methacrylamide and subsequent quaternization with o-nitrobenzyl 2-chloroacetate. The photolabile o-nitrobenzyl carboxymethyl pendant moiety is transformed to the zwitterionic carboxybetaine form upon the irradiation at 365 nm. This feature is used to condense and, upon the light irradiation, to release double-strand DNA tested by gel electrophoresis and surface plasmon resonance experiments as well as to switch the antibacterial activity to non-toxic character demonstrated for Escherichia coli bacterial cells in solution and at the surface using the self-assembled monolayers.

Citovat

SOBOLČIAK, Patrik, Mário ŠPÍREK, Jaroslav KATRLÍK, Peter GEMEINER, Igor LACÍK a Peter KASÁK. Light-Switchable Polymer from Cationic to Zwitterionic Form: Synthesis, Characterization, and Interactions with DNA and Bacterial Cells. MACROMOLECULAR RAPID COMMUNICATIONS. 2013, roč. 34, č. 8, s. 635-639. ISSN 1022-1336. Dostupné z: https://dx.doi.org/10.1002/marc.201200823.

@article{1170156,
   author = {Sobolčiak, Patrik and Špírek, Mário and Katrlík, Jaroslav and Gemeiner, Peter and Lacík, Igor and Kasák, Peter},
   article_number = {8},
   doi = {http://dx.doi.org/10.1002/marc.201200823},
   keywords = {antibacterial; carboxybetaine ester; dsDNA polyplex; photochemistry; water-soluble polymers},
   language = {eng},
   issn = {1022-1336},
   journal = {MACROMOLECULAR RAPID COMMUNICATIONS},
   title = {Light-Switchable Polymer from Cationic to Zwitterionic Form: Synthesis, Characterization, and Interactions with DNA and Bacterial Cells},
   volume = {34},
   year = {2013}
}

TY  - JOUR
ID  - 1170156
AU  - Sobolčiak, Patrik - Špírek, Mário - Katrlík, Jaroslav - Gemeiner, Peter - Lacík, Igor - Kasák, Peter
PY  - 2013
TI  - Light-Switchable Polymer from Cationic to Zwitterionic Form: Synthesis, Characterization, and Interactions with DNA and Bacterial Cells
JF  - MACROMOLECULAR RAPID COMMUNICATIONS
VL  - 34
IS  - 8
SP  - 635-639
EP  - 635-639
SN  - 10221336
KW  - antibacterial
KW  - carboxybetaine ester
KW  - dsDNA polyplex
KW  - photochemistry
KW  - water-soluble polymers
N2  - A novel cationic polymer poly(N,N-dimethyl-N-[3-(methacroylamino) propyl]-N-[2-[(2-nitrophenyl)methoxy]-2-oxo-ethyl]ammonium chloride) is synthesized by free-radical polymerization of N-[3-(dimethylamino)propyl] methacrylamide and subsequent quaternization with o-nitrobenzyl 2-chloroacetate. The photolabile o-nitrobenzyl carboxymethyl pendant moiety is transformed to the zwitterionic carboxybetaine form upon the irradiation at 365 nm. This feature is used to condense and, upon the light irradiation, to release double-strand DNA tested by gel electrophoresis and surface plasmon resonance experiments as well as to switch the antibacterial activity to non-toxic character demonstrated for Escherichia coli bacterial cells in solution and at the surface using the self-assembled monolayers.
ER  -

SOBOLČIAK, Patrik, Mário ŠPÍREK, Jaroslav KATRLÍK, Peter GEMEINER, Igor LACÍK a Peter KASÁK. Light-Switchable Polymer from Cationic to Zwitterionic Form: Synthesis, Characterization, and Interactions with DNA and Bacterial Cells. \textit{MACROMOLECULAR RAPID COMMUNICATIONS}. 2013, roč.~34, č.~8, s.~635-639. ISSN~1022-1336. Dostupné z: https://dx.doi.org/10.1002/marc.201200823.

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