VAN4 Encodes a Putative TRS120 That is Required for Normal Cell
Growth and Vein Development in Arabidopsis

J 2014

VAN4 Encodes a Putative TRS120 That is Required for Normal Cell Growth and Vein Development in Arabidopsis

NARAMOTO, S., Tomasz NODZYNSKI, T. DAINOBU, H. TAKATSUKA, T. OKADA et. al.

Základní údaje

Originální název

VAN4 Encodes a Putative TRS120 That is Required for Normal Cell Growth and Vein Development in Arabidopsis

Autoři

NARAMOTO, S. (392 Japonsko), Tomasz NODZYNSKI (616 Polsko, domácí), T. DAINOBU (392 Japonsko), H. TAKATSUKA (392 Japonsko), T. OKADA (392 Japonsko), Jiří FRIML (203 Česká republika, garant, domácí) a H. FUKUDA (392 Japonsko)

Vydání

PLANT AND CELL PHYSIOLOGY, Oxford, Oxford University Press, 2014, 0032-0781

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.931

Kód RIV

RIV/00216224:14740/14:00073545

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1093/pcp/pcu012

UT WoS

000334679500009

Klíčová slova anglicky

Arabidopsis; Cell growth; Guanine nucleotide exchange factor (GEF); Leaf venation pattern; Vascular cell differentiation; Vesicle trafficking

Štítky

kontrola MP, ok, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 3. 2015 11:24, Martina Prášilová

Anotace

V originále

Leaf venation develops complex patterns in angiosperms, but the mechanism underlying this process is largely unknown. To elucidate the molecular mechanisms governing vein pattern formation, we previously isolated vascular network defective (van) mutants that displayed venation discontinuities. Here, we report the phenotypic analysis of van4 mutants, and we identify and characterize the VAN4 gene. Detailed phenotypic analysis shows that van4 mutants are defective in procambium cell differentiation and subsequent vascular cell differentiation. Reduced shoot and root cell growth is observed in van4 mutants, suggesting that VAN4 function is important for cell growth and the establishment of venation continuity. Consistent with these phenotypes, the VAN4 gene is strongly expressed in vascular and meristematic cells. VAN4 encodes a putative TRS120, which is a known guanine nucleotide exchange factor (GEF) for Rab GTPase involved in regulating vesicle transport, and a known tethering factor that determines the specificity of membrane fusion. VAN4 protein localizes at the trans-Golgi network/early endosome (TGN/EE). Aberrant recycling of the auxin efflux carrier PIN proteins is observed in van4 mutants. These results suggest that VAN4-mediated exocytosis at the TGN plays important roles in plant vascular development and cell growth in shoot and root. Our identification of VAN4 as a putative TRS120 shows that Rab GTPases are crucial (in addition to ARF GTPases) for continuous vascular development, and provides further evidence for the importance of vesicle transport in leaf vascular formation.

Návaznosti

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GA13-40637S, projekt VaV

Název: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Grantová agentura ČR, Genetické studie k identifikaci molekulárních mechanizmu bunecné polarity a auxinového transportu v rostlinách

Citovat

NARAMOTO, S., Tomasz NODZYNSKI, T. DAINOBU, H. TAKATSUKA, T. OKADA, Jiří FRIML a H. FUKUDA. VAN4 Encodes a Putative TRS120 That is Required for Normal Cell Growth and Vein Development in Arabidopsis. PLANT AND CELL PHYSIOLOGY. Oxford: Oxford University Press, 2014, roč. 55, č. 4, s. 750-763. ISSN 0032-0781. Dostupné z: https://dx.doi.org/10.1093/pcp/pcu012.

@article{1173185,
   author = {Naramoto, S. and Nodzynski, Tomasz and Dainobu, T. and Takatsuka, H. and Okada, T. and Friml, Jiří and Fukuda, H.},
   article_location = {Oxford},
   article_number = {4},
   doi = {http://dx.doi.org/10.1093/pcp/pcu012},
   keywords = {Arabidopsis; Cell growth; Guanine nucleotide exchange factor (GEF); Leaf venation pattern; Vascular cell differentiation; Vesicle trafficking},
   language = {eng},
   issn = {0032-0781},
   journal = {PLANT AND CELL PHYSIOLOGY},
   title = {VAN4 Encodes a Putative TRS120 That is Required for Normal Cell Growth and Vein Development in Arabidopsis},
   url = {http://pcp.oxfordjournals.org/content/55/4/750.long},
   volume = {55},
   year = {2014}
}

TY  - JOUR
ID  - 1173185
AU  - Naramoto, S. - Nodzynski, Tomasz - Dainobu, T. - Takatsuka, H. - Okada, T. - Friml, Jiří - Fukuda, H.
PY  - 2014
TI  - VAN4 Encodes a Putative TRS120 That is Required for Normal Cell Growth and Vein Development in Arabidopsis
JF  - PLANT AND CELL PHYSIOLOGY
VL  - 55
IS  - 4
SP  - 750-763
EP  - 750-763
PB  - Oxford University Press
SN  - 00320781
KW  - Arabidopsis
KW  - Cell growth
KW  - Guanine nucleotide exchange factor (GEF)
KW  - Leaf venation pattern
KW  - Vascular cell differentiation
KW  - Vesicle trafficking
UR  - http://pcp.oxfordjournals.org/content/55/4/750.long
L2  - http://pcp.oxfordjournals.org/content/55/4/750.long
N2  - Leaf venation develops complex patterns in angiosperms, but the mechanism underlying this process is largely unknown. To elucidate the molecular mechanisms governing vein pattern formation, we previously isolated vascular network defective (van) mutants that displayed venation discontinuities. Here, we report the phenotypic analysis of van4 mutants, and we identify and characterize the VAN4 gene. Detailed phenotypic analysis shows that van4 mutants are defective in procambium cell differentiation and subsequent vascular cell differentiation. Reduced shoot and root cell growth is observed in van4 mutants, suggesting that VAN4 function is important for cell growth and the establishment of venation continuity. Consistent with these phenotypes, the VAN4 gene is strongly expressed in vascular and meristematic cells. VAN4 encodes a putative TRS120, which is a known guanine nucleotide exchange factor (GEF) for Rab GTPase involved in regulating vesicle transport, and a known tethering factor that determines the specificity of membrane fusion. VAN4 protein localizes at the trans-Golgi network/early endosome (TGN/EE). Aberrant recycling of the auxin efflux carrier PIN proteins is observed in van4 mutants. These results suggest that VAN4-mediated exocytosis at the TGN plays important roles in plant vascular development and cell growth in shoot and root. Our identification of VAN4 as a putative TRS120 shows that Rab GTPases are crucial (in addition to ARF GTPases) for continuous vascular development, and provides further evidence for the importance of vesicle transport in leaf vascular formation.
ER  -

NARAMOTO, S., Tomasz NODZYNSKI, T. DAINOBU, H. TAKATSUKA, T. OKADA, Jiří FRIML a H. FUKUDA. VAN4 Encodes a Putative TRS120 That is Required for Normal Cell Growth and Vein Development in Arabidopsis. \textit{PLANT AND CELL PHYSIOLOGY}. Oxford: Oxford University Press, 2014, roč.~55, č.~4, s.~750-763. ISSN~0032-0781. Dostupné z: https://dx.doi.org/10.1093/pcp/pcu012.

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