Selected phenotypic features of BR91, a unique spirochaetal
strain isolated from the Culex pipiens mosquito

J 2014

Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito

SIKUTOVÁ, Silvie; Leona BUNKOVÁ; Eva KREJCÍ; Jiří HALOUZKA; Yibayiri O. SANOGO et. al.

Základní údaje

Originální název

Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito

Autoři

SIKUTOVÁ, Silvie (203 Česká republika); Leona BUNKOVÁ (203 Česká republika); Eva KREJCÍ (203 Česká republika); Jiří HALOUZKA (203 Česká republika); Yibayiri O. SANOGO (840 Spojené státy) a Ivo RUDOLF (203 Česká republika, garant, domácí)

Vydání

Microbiological Research, 2014, 0944-5013

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.561

Kód RIV

RIV/00216224:14310/14:00081992

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.micres.2013.10.002

UT WoS

000335107900003

EID Scopus

2-s2.0-84896545445

Klíčová slova anglicky

Spirochaete; Culex pipiens; Enzyme activity; SDS-PAGE; FAME

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 4. 2016 13:00, Ing. Andrea Mikešková

Anotace

V originále

Growth temperature range, resistance to selective antibiotics, activities of 23 enzymes, protein fingerprints and fatty acids composition of the spirochaetal strain BR91, isolated from the Culex pipiens mosquito, were tested. The spirochaetes were grown in BSK-H Complete liquid medium. The optimal in vitro growth temperature of the strain was 33 °C. Strain BR91 was sensitive to trimethoprim, nalidixic acid, 5-fluorouracil, and tolerated phosphomycin. The strain produced acid and alkaline phosphatase, esterase (C4), esterase–lipase (C8), leucine arylamidase, naphthol-AS-BI-phosphohydrolase and alpha-fucosidase. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay revealed several major proteins in the size range of 13–16 kDa, 22–30 kDa and 37–131 kDa. Fatty acid methyl ester (FAME) analysis showed that C14:0, C16:0, C18:1 etha9c and summed feature 5 (C18:2 etha6,9c and/or C18:0 anteiso) are major fatty acids. This study highlights certain phenotypic differences between strain BR91 and the Lyme disease spirochaete Borrelia burgdorferi, and supports the hypothesis that strain BR91 represents a unique taxonomical entity in a system of spirochaetal species.

Návaznosti

EE2.3.20.0183, projekt VaV

Název: Centrum experimentální biomedicíny

Citovat

SIKUTOVÁ, Silvie; Leona BUNKOVÁ; Eva KREJCÍ; Jiří HALOUZKA; Yibayiri O. SANOGO a Ivo RUDOLF. Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito. Microbiological Research. 2014, roč. 169, 5-6, s. 348-352. ISSN 0944-5013. Dostupné z: https://dx.doi.org/10.1016/j.micres.2013.10.002.

@article{1173333,
   author = {Sikutová, Silvie and Bunková, Leona and Krejcí, Eva and Halouzka, Jiří and Sanogo, Yibayiri O. and Rudolf, Ivo},
   article_number = {5-6},
   doi = {http://dx.doi.org/10.1016/j.micres.2013.10.002},
   keywords = {Spirochaete; Culex pipiens; Enzyme activity; SDS-PAGE; FAME},
   language = {eng},
   issn = {0944-5013},
   journal = {Microbiological Research},
   title = {Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito},
   volume = {169},
   year = {2014}
}

TY  - JOUR
ID  - 1173333
AU  - Sikutová, Silvie - Bunková, Leona - Krejcí, Eva - Halouzka, Jiří - Sanogo, Yibayiri O. - Rudolf, Ivo
PY  - 2014
TI  - Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito
JF  - Microbiological Research
VL  - 169
IS  - 5-6
SP  - 348-352
EP  - 348-352
SN  - 09445013
KW  - Spirochaete
KW  - Culex pipiens
KW  - Enzyme activity
KW  - SDS-PAGE
KW  - FAME
N2  - Growth temperature range, resistance to selective antibiotics, activities of 23 enzymes, protein fingerprints and fatty acids composition of the spirochaetal strain BR91, isolated from the Culex pipiens mosquito, were tested. The spirochaetes were grown in BSK-H Complete liquid medium. The optimal in vitro growth temperature of the strain was 33 °C. Strain BR91 was sensitive to trimethoprim, nalidixic acid, 5-fluorouracil, and tolerated phosphomycin. The strain produced acid and alkaline phosphatase, esterase (C4), esterase–lipase (C8), leucine arylamidase, naphthol-AS-BI-phosphohydrolase and alpha-fucosidase. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay revealed several major proteins in the size range of 13–16 kDa, 22–30 kDa and 37–131 kDa. Fatty acid methyl ester (FAME) analysis showed that C14:0, C16:0, C18:1 etha9c and summed feature 5 (C18:2 etha6,9c and/or C18:0 anteiso) are major fatty acids. This study highlights certain phenotypic differences between strain BR91 and the Lyme disease spirochaete Borrelia burgdorferi, and supports the hypothesis that strain BR91 represents a unique taxonomical entity in a system of spirochaetal species.
ER  -

SIKUTOVÁ, Silvie; Leona BUNKOVÁ; Eva KREJCÍ; Jiří HALOUZKA; Yibayiri O. SANOGO a Ivo RUDOLF. Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito. \textit{Microbiological Research}. 2014, roč.~169, 5-6, s.~348-352. ISSN~0944-5013. Dostupné z: https://dx.doi.org/10.1016/j.micres.2013.10.002.

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