beta-Arrestin Interacts with the Beta/Gamma Subunits of
Trimeric G-Proteins and Dishevelled in the Wnt/Ca2+ Pathway in
Xenopus Gastrulation

J 2014

beta-Arrestin Interacts with the Beta/Gamma Subunits of Trimeric G-Proteins and Dishevelled in the Wnt/Ca2+ Pathway in Xenopus Gastrulation

SEITZ, Katharina; Verena DUERSCH; Jakub HARNOŠ; Vítězslav BRYJA; Marc GENTZEL et al.

Základní údaje

Originální název

beta-Arrestin Interacts with the Beta/Gamma Subunits of Trimeric G-Proteins and Dishevelled in the Wnt/Ca2+ Pathway in Xenopus Gastrulation

Autoři

SEITZ, Katharina; Verena DUERSCH; Jakub HARNOŠ; Vítězslav BRYJA; Marc GENTZEL a Alexandra SCHAMBONY

Vydání

PLOS ONE, 2014, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.234

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/14:00073567

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

CONVERGENT EXTENSION MOVEMENTS; WNT SIGNALING PATHWAYS; WNT/BETA-CATENIN; VERTEBRATE GASTRULATION; PARAXIAL PROTOCADHERIN; TISSUE SEPARATION; ACTIVATION; FRIZZLED-7; LAEVIS; KINASE

Štítky

AKR, rivok

Změněno: 10. 3. 2015 14:49, Mgr. et Mgr. Veronika Oškerová, Ph.D.

Anotace

V originále

beta-Catenin independent, non-canonical Wnt signaling pathways play a major role in the regulation of morphogenetic movements in vertebrates. The term non-canonical Wnt signaling comprises multiple, intracellularly divergent, Wnt-activated and beta-Catenin independent signaling cascades including the Wnt/Planar Cell Polarity and the Wnt/Ca2+ cascades. Wnt/Planar Cell Polarity and Wnt/Ca2+ pathways share common effector proteins, including the Wnt ligand, Frizzled receptors and Dishevelled, with each other and with additional branches of Wnt signaling. Along with the aforementioned proteins, beta-Arrestin has been identified as an essential effector protein in the Wnt/beta-Catenin and the Wnt/Planar Cell Polarity pathway. Our results demonstrate that beta-Arrestin is required in the Wnt/Ca2+ signaling cascade upstream of Protein Kinase C (PKC) and Ca2+/Calmodulin-dependent Protein Kinase II (CamKII). We have further characterized the role of beta-Arrestin in this branch of non-canonical Wnt signaling by knock-down and rescue experiments in Xenopus embryo explants and analyzed protein-protein interactions in 293T cells. Functional interaction of beta-Arrestin, the beta subunit of trimeric G-proteins and Dishevelled is required to induce PKC activation and membrane translocation. In Xenopus gastrulation, beta-Arrestin function in Wnt/Ca2+ signaling is essential for convergent extension movements. We further show that beta-Arrestin physically interacts with the beta subunit of trimeric G-proteins and Dishevelled, and that the interaction between beta-Arrestin and Dishevelled is promoted by the beta/gamma subunits of trimeric G-proteins, indicating the formation of a multiprotein signaling complex.

Návaznosti

GA204/09/0498, projekt VaV

Název: Dynamika proteinů interagujících s Dishevelled a jejich význam pro Wnt signálování

Investor: Grantová agentura ČR, Dynamika proteinů interagujících s Dishevelled a jejich význam pro Wnt signálování

MSM0021622430, záměr

Název: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Citovat

SEITZ, Katharina; Verena DUERSCH; Jakub HARNOŠ; Vítězslav BRYJA; Marc GENTZEL a Alexandra SCHAMBONY. beta-Arrestin Interacts with the Beta/Gamma Subunits of Trimeric G-Proteins and Dishevelled in the Wnt/Ca2+ Pathway in Xenopus Gastrulation. PLOS ONE. 2014, roč. 9, č. 1, s. "nestrankovano", 11 s. ISSN 1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0087132.

@article{1173919,
   author = {Seitz, Katharina and Duersch, Verena and Harnoš, Jakub and Bryja, Vítězslav and Gentzel, Marc and Schambony, Alexandra},
   article_number = {1},
   doi = {https://doi.org/10.1371/journal.pone.0087132},
   keywords = {CONVERGENT EXTENSION MOVEMENTS; WNT SIGNALING PATHWAYS; WNT/BETA-CATENIN; VERTEBRATE GASTRULATION; PARAXIAL PROTOCADHERIN; TISSUE SEPARATION; ACTIVATION; FRIZZLED-7; LAEVIS; KINASE},
   language = {eng},
   issn = {1932-6203},
   journal = {PLOS ONE},
   title = {beta-Arrestin Interacts with the Beta/Gamma Subunits of Trimeric G-Proteins and Dishevelled in the Wnt/Ca2+ Pathway in Xenopus Gastrulation},
   url = {http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=17&SID=R2g9W8Fv71CDiX3zjH6&page=1&doc=1#},
   volume = {9},
   year = {2014}
}

TY  - JOUR
ID  - 1173919
AU  - Seitz, Katharina - Duersch, Verena - Harnoš, Jakub - Bryja, Vítězslav - Gentzel, Marc - Schambony, Alexandra
PY  - 2014
TI  - beta-Arrestin Interacts with the Beta/Gamma Subunits of Trimeric G-Proteins and Dishevelled in the Wnt/Ca2+ Pathway in Xenopus Gastrulation
JF  - PLOS ONE
VL  - 9
IS  - 1
SP  - "nestrankovano"
EP  - "nestrankovano"
SN  - 19326203
KW  - CONVERGENT EXTENSION MOVEMENTS
KW  - WNT SIGNALING PATHWAYS
KW  - WNT/BETA-CATENIN
KW  - VERTEBRATE GASTRULATION
KW  - PARAXIAL PROTOCADHERIN
KW  - TISSUE SEPARATION
KW  - ACTIVATION
KW  - FRIZZLED-7
KW  - LAEVIS
KW  - KINASE
UR  - http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=17&SID=R2g9W8Fv71CDiX3zjH6&page=1&doc=1#
N2  - beta-Catenin independent, non-canonical Wnt signaling pathways play a major role in the regulation of morphogenetic movements in vertebrates. The term non-canonical Wnt signaling comprises multiple, intracellularly divergent, Wnt-activated and beta-Catenin independent signaling cascades including the Wnt/Planar Cell Polarity and the Wnt/Ca2+ cascades. Wnt/Planar Cell Polarity and Wnt/Ca2+ pathways share common effector proteins, including the Wnt ligand, Frizzled receptors and Dishevelled, with each other and with additional branches of Wnt signaling. Along with the aforementioned proteins, beta-Arrestin has been identified as an essential effector protein in the Wnt/beta-Catenin and the Wnt/Planar Cell Polarity pathway. Our results demonstrate that beta-Arrestin is required in the Wnt/Ca2+ signaling cascade upstream of Protein Kinase C (PKC) and Ca2+/Calmodulin-dependent Protein Kinase II (CamKII). We have further characterized the role of beta-Arrestin in this branch of non-canonical Wnt signaling by knock-down and rescue experiments in Xenopus embryo explants and analyzed protein-protein interactions in 293T cells. Functional interaction of beta-Arrestin, the beta subunit of trimeric G-proteins and Dishevelled is required to induce PKC activation and membrane translocation. In Xenopus gastrulation, beta-Arrestin function in Wnt/Ca2+ signaling is essential for convergent extension movements. We further show that beta-Arrestin physically interacts with the beta subunit of trimeric G-proteins and Dishevelled, and that the interaction between beta-Arrestin and Dishevelled is promoted by the beta/gamma subunits of trimeric G-proteins, indicating the formation of a multiprotein signaling complex.
ER  -

SEITZ, Katharina; Verena DUERSCH; Jakub HARNOŠ; Vítězslav BRYJA; Marc GENTZEL a Alexandra SCHAMBONY. beta-Arrestin Interacts with the Beta/Gamma Subunits of Trimeric G-Proteins and Dishevelled in the Wnt/Ca2+ Pathway in Xenopus Gastrulation. \textit{PLOS ONE}. 2014, roč.~9, č.~1, s.~''nestrankovano'', 11 s. ISSN~1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0087132.

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