Influence of Salmonella enterica serovar Enteritidis infection
on the composition of chicken cecal microbiota

J 2013

Influence of Salmonella enterica serovar Enteritidis infection on the composition of chicken cecal microbiota

VÍDEŇSKÁ, Petra; F SISAK; H HAVLICKOVA; M FALDYNOVA; Ivan RYCHLIK et al.

Základní údaje

Originální název

Influence of Salmonella enterica serovar Enteritidis infection on the composition of chicken cecal microbiota

Autoři

VÍDEŇSKÁ, Petra; F SISAK; H HAVLICKOVA; M FALDYNOVA a Ivan RYCHLIK

Vydání

BMC Veterinary Research, LONDON, BIOMED CENTRAL LTD, 2013, 1746-6148

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.743

Označené pro přenos do RIV

DOI

https://doi.org/10.1186/1746-6148-9-140

UT WoS

000322043900001

Klíčová slova anglicky

Chicken; Microbiome; Intestinal tract; Pyrosequencing; Salmonella

Příznaky

Recenzováno

Změněno: 4. 4. 2014 08:09, Mgr. Petra Vídeňská, Ph.D.

Anotace

V originále

Background: Infection of newly hatched chicks with Salmonella enterica serovar Enteritidis (S. Enteritidis) results in an inflammatory response in the intestinal tract which may influence the composition of gut microbiota. In this study we were therefore interested whether S. Enteritidis induced inflammation results in changes in the cecal microbiota. To reach this aim, we compared the cecal microbiota of non-infected chickens and those infected by S. Enteritidis by pyrosequencing the V3/V4 variable regions of genes coding for 16S rRNA. Results: Cecal microbiota of chickens up to 19 days of life was dominated by representatives of Enterobacteriaceae, Lachnospiraceae and Ruminococcaceae, followed by Lactobacillaceae. The presence of Lachnospiraceae did not change after S. Enteritidis infection. Enterobacteriaceae increased and Ruminococcaceae decreased after S. Enteritidis infection in two independent experiments although these results were not significant. A significant increase in both experiments was observed only for the representatives of Lactobacillaceae which may correlate with their microaerophilic growth characteristic compared to the obligate anaerobes from the families Lachnospiraceae and Ruminococcaceae. Conclusions: We conclude that S. Enteritidis infection influences the composition of the cecal microbiota in chickens but these changes are minor in nature and should be understood more as an indirect consequence of infection and inflammation rather than a positively selected evolutionary trait.

Citovat

VÍDEŇSKÁ, Petra; F SISAK; H HAVLICKOVA; M FALDYNOVA a Ivan RYCHLIK. Influence of Salmonella enterica serovar Enteritidis infection on the composition of chicken cecal microbiota. BMC Veterinary Research. LONDON: BIOMED CENTRAL LTD, 2013, roč. 9, 8 s. ISSN 1746-6148. Dostupné z: https://doi.org/10.1186/1746-6148-9-140.

@article{1174128,
   author = {Vídeňská, Petra and Sisak, F and Havlickova, H and Faldynova, M and Rychlik, Ivan},
   article_location = {LONDON},
   doi = {https://doi.org/10.1186/1746-6148-9-140},
   keywords = {Chicken; Microbiome; Intestinal tract; Pyrosequencing; Salmonella},
   language = {eng},
   issn = {1746-6148},
   journal = {BMC Veterinary Research},
   title = {Influence of Salmonella enterica serovar Enteritidis infection on the composition of chicken cecal microbiota},
   volume = {9},
   year = {2013}
}

TY  - JOUR
ID  - 1174128
AU  - Vídeňská, Petra - Sisak, F - Havlickova, H - Faldynova, M - Rychlik, Ivan
PY  - 2013
TI  - Influence of Salmonella enterica serovar Enteritidis infection on the composition of chicken cecal microbiota
JF  - BMC Veterinary Research
VL  - 9
PB  - BIOMED CENTRAL LTD
SN  - 17466148
KW  - Chicken
KW  - Microbiome
KW  - Intestinal tract
KW  - Pyrosequencing
KW  - Salmonella
N2  - Background: Infection of newly hatched chicks with Salmonella enterica serovar Enteritidis (S. Enteritidis) results in an inflammatory response in the intestinal tract which may influence the composition of gut microbiota. In this study we were therefore interested whether S. Enteritidis induced inflammation results in changes in the cecal microbiota. To reach this aim, we compared the cecal microbiota of non-infected chickens and those infected by S. Enteritidis by pyrosequencing the V3/V4 variable regions of genes coding for 16S rRNA. Results: Cecal microbiota of chickens up to 19 days of life was dominated by representatives of Enterobacteriaceae, Lachnospiraceae and Ruminococcaceae, followed by Lactobacillaceae. The presence of Lachnospiraceae did not change after S. Enteritidis infection. Enterobacteriaceae increased and Ruminococcaceae decreased after S. Enteritidis infection in two independent experiments although these results were not significant. A significant increase in both experiments was observed only for the representatives of Lactobacillaceae which may correlate with their microaerophilic growth characteristic compared to the obligate anaerobes from the families Lachnospiraceae and Ruminococcaceae. Conclusions: We conclude that S. Enteritidis infection influences the composition of the cecal microbiota in chickens but these changes are minor in nature and should be understood more as an indirect consequence of infection and inflammation rather than a positively selected evolutionary trait.
ER  -

VÍDEŇSKÁ, Petra; F SISAK; H HAVLICKOVA; M FALDYNOVA a Ivan RYCHLIK. Influence of Salmonella enterica serovar Enteritidis infection on the composition of chicken cecal microbiota. \textit{BMC Veterinary Research}. LONDON: BIOMED CENTRAL LTD, 2013, roč.~9, 8 s. ISSN~1746-6148. Dostupné z: https://doi.org/10.1186/1746-6148-9-140.

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