Detailed Information on Publication Record
2013
Microscopic visualization of the body organisation in diplozoids
HODOVÁ, Iveta, Milan GELNAR and Andrea VALIGUROVÁBasic information
Original name
Microscopic visualization of the body organisation in diplozoids
Authors
HODOVÁ, Iveta (203 Czech Republic, guarantor, belonging to the institution), Milan GELNAR (203 Czech Republic, belonging to the institution) and Andrea VALIGUROVÁ (703 Slovakia, belonging to the institution)
Edition
2013
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
Brazil
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/13:00067052
Organization unit
Faculty of Science
Keywords in English
Monogenea; Diplozoidae
Tags
International impact
Změněno: 4/4/2014 14:22, Mgr. Iveta Hodová, Ph.D.
Abstract
V originále
Diplozoidae represent blood-feeding gill ectoparasites of freshwater fish. They exhibit extraordinary morphology and life cycle involving permanent fusion of two larval worms and their subsequent transformation into one individual (permanent copula). Different developmental stages including diporpa, juvenile and adult of 3 species Paradiplozoon homoion, Paradiplozoon blicae and Eudiplozoon nipponicum were investigated for the body organisation and its adaptation to the ectoparasitic life. Using a combined approach of light, confocal and electron microscopy, this study focused on the parasite attachment to and localization on the host body. Special attention was given to parasite’s digestive, reproductive and excretory systems. The attachment apparatus along with attached muscle bundles forms during parasite’s development, which is clearly visible under the confocal microscope and in histological sections. Labelling of filamentous actin with fluorescent phaloidin revealed the musculature of the body wall, buccal suckers, the pharynx as well as some parts of reproductive tract. Staining with chloride carmine and counterstaining with DAPI proved to be powerful tools for confocal microscopic detection of various cell types including putative gland cells. A preliminary immunofluorescent detection of alfa-tubulin showed to be suitable for visualisation of excretory system, some parts of nervous system and spermatozoons. In addition, a strong autofluorescence signal, excited in the green wavelengths, facilitated visualization of egg and vitelloduct reservoir without specific staining.
Links
| GAP506/12/1258, research and development project |
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| GBP505/12/G112, research and development project |
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