Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne
asmulti-redox centre DNA label at mercury meniscus modified
silversolid amalgam electrode

J 2014

Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode

DAŇHEL, Aleš; Veronika RAINDLOVA; Luděk HAVRAN; Hana PIVOŇKOVÁ; Michal HOCEK et. al.

Základní údaje

Originální název

Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode

Autoři

DAŇHEL, Aleš; Veronika RAINDLOVA; Luděk HAVRAN; Hana PIVOŇKOVÁ; Michal HOCEK a Miroslav FOJTA

Vydání

Electrochimica Acta, OXFORD, Elsevier Science Ltd, 2014, 0013-4686

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.504

Kód RIV

RIV/00216224:14740/14:00073589

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1016/j.electacta.2013.09.147

UT WoS

000336340900016

EID Scopus

2-s2.0-84897982345

Klíčová slova anglicky

DNA; Redox labeling; 2_4-Dinitrophenylhydrazine; Silver solid amalgam electrode; Voltammetry

Štítky

DNA, kontrola MP, rivok, Voltammetry

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 3. 2015 08:30, Martina Prášilová

Anotace

V originále

A detailed voltammetric study of 2,4-dinitrophenylhydrazine (DNPH), a DNPH modified nucleotide(dCDNPHMP) and a short single stranded DNA (ssDNA-DNPH) at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) is presented in this work. Electrochemical behaviour of these compounds was studied using cyclic voltammetry (CV), adsorptive stripping CV and open circuit adsorptive transfer stripping CV. Successful incorpo-ration of the DNPH labeled nucleoside triphosphate into the DNA structure shows that selected DNApolymerase tolerates this bulky moiety and can be used, in combination with electrochemical methodsutilizing m-AgSAE, in further development of various applications.

Návaznosti

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

DAŇHEL, Aleš; Veronika RAINDLOVA; Luděk HAVRAN; Hana PIVOŇKOVÁ; Michal HOCEK a Miroslav FOJTA. Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode. Electrochimica Acta. OXFORD: Elsevier Science Ltd, 2014, roč. 126, SI, s. 122-131. ISSN 0013-4686. Dostupné z: https://doi.org/10.1016/j.electacta.2013.09.147.

@article{1179581,
   author = {Daňhel, Aleš and Raindlova, Veronika and Havran, Luděk and Pivoňková, Hana and Hocek, Michal and Fojta, Miroslav},
   article_location = {OXFORD},
   article_number = {SI},
   doi = {https://doi.org/10.1016/j.electacta.2013.09.147},
   keywords = {DNA; Redox labeling; 2_4-Dinitrophenylhydrazine; Silver solid amalgam electrode; Voltammetry},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode},
   url = {http://dx.doi.org/10.1016/j.electacta.2013.09.147},
   volume = {126},
   year = {2014}
}

TY  - JOUR
ID  - 1179581
AU  - Daňhel, Aleš - Raindlova, Veronika - Havran, Luděk - Pivoňková, Hana - Hocek, Michal - Fojta, Miroslav
PY  - 2014
TI  - Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode
JF  - Electrochimica Acta
VL  - 126
IS  - SI
SP  - 122-131
EP  - 122-131
PB  - Elsevier Science Ltd
SN  - 00134686
KW  - DNA
KW  - Redox labeling
KW  - 2_4-Dinitrophenylhydrazine
KW  - Silver solid amalgam electrode
KW  - Voltammetry
UR  - http://dx.doi.org/10.1016/j.electacta.2013.09.147
L2  - http://dx.doi.org/10.1016/j.electacta.2013.09.147
N2  - A detailed voltammetric study of 2,4-dinitrophenylhydrazine (DNPH), a DNPH modified nucleotide(dCDNPHMP) and a short single stranded DNA (ssDNA-DNPH) at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) is presented in this work. Electrochemical behaviour of these compounds was studied using cyclic voltammetry (CV), adsorptive stripping CV and open circuit adsorptive transfer stripping CV. Successful incorpo-ration of the DNPH labeled nucleoside triphosphate into the DNA structure shows that selected DNApolymerase tolerates this bulky moiety and can be used, in combination with electrochemical methodsutilizing m-AgSAE, in further development of various applications.
ER  -

DAŇHEL, Aleš; Veronika RAINDLOVA; Luděk HAVRAN; Hana PIVOŇKOVÁ; Michal HOCEK a Miroslav FOJTA. Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode. \textit{Electrochimica Acta}. OXFORD: Elsevier Science Ltd, 2014, roč.~126, SI, s.~122-131. ISSN~0013-4686. Dostupné z: https://doi.org/10.1016/j.electacta.2013.09.147.

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