Circular dichroism and conformational polymorphism of DNA

KYPR, Jaroslav, Iva KEJNOVSKÁ, Daniel RENČIUK a Michaela VORLÍČKOVÁ. Circular dichroism and conformational polymorphism of DNA. Nucleic Acids Research. Oxford: Oxford Press, 2009, roč. 37, č. 6, s. 1713-1725. ISSN 0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkp026.

Základní údaje

• Originální název: Circular dichroism and conformational polymorphism of DNA
• Autoři: KYPR, Jaroslav, Iva KEJNOVSKÁ, Daniel RENČIUK a Michaela VORLÍČKOVÁ.
• Vydání: Nucleic Acids Research, Oxford, Oxford Press, 2009, 0305-1048.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: 10600 1.6 Biological sciences
• Stát vydavatele: Velká Británie a Severní Irsko
• Utajení: není předmětem státního či obchodního tajemství
• Impakt faktor: Impact factor: 7.479
• Organizační jednotka: Středoevropský technologický institut
• Doi: http://dx.doi.org/10.1093/nar/gkp026
• UT WoS: 000265097400008
• Klíčová slova anglicky: GUANINE-GUANINE STACKING; HUMAN TELOMERIC SEQUENCE; ADENINE REPEAT STRANDS; DOUBLE-HELICAL DNA; G-QUADRUPLEX; NUCLEIC-ACID; POTASSIUM SOLUTION; CRYSTAL-STRUCTURE; MELTING CURVES; SYNTHETIC DNA
• Štítky: ok

Anotace

Here we review studies that provided important information about conformational properties of DNA using circular dichroic (CD) spectroscopy. The conformational properties include the B-family of structures, A-form, Z-form, guanine quadruplexes, cytosine quadruplexes, triplexes and other less characterized structures. CD spectroscopy is extremely sensitive and relatively inexpensive. This fast and simple method can be used at low- as well as high-DNA concentrations and with short- as well as long-DNA molecules. The samples can easily be titrated with various agents to cause conformational isomerizations of DNA. The course of detected CD spectral changes makes possible to distinguish between gradual changes within a single DNA conformation and cooperative isomerizations between discrete structural states. It enables measuring kinetics of the appearance of particular conformers and determination of their thermodynamic parameters. In careful hands, CD spectroscopy is a valuable tool for mapping conformational properties of particular DNA molecules. Due to its numerous advantages, CD spectroscopy significantly participated in all basic conformational findings on DNA.