a 2014

Cysteine peptidase inhibitors of helminths

ILGOVÁ, Jana, Milan GELNAR, Břetislav KOUDELA and Martin KAŠNÝ

Basic information

Original name

Cysteine peptidase inhibitors of helminths

Authors

ILGOVÁ, Jana (703 Slovakia, guarantor, belonging to the institution), Milan GELNAR (203 Czech Republic, belonging to the institution), Břetislav KOUDELA (203 Czech Republic, belonging to the institution) and Martin KAŠNÝ (203 Czech Republic, belonging to the institution)

Edition

XXI. Helminthological Days, 2014

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/14:00073621

Organization unit

Faculty of Science

ISBN

978-80-210-6796-7

Keywords in English

cysteine peptidase inhibitors; helminths; immunomodulation
Změněno: 12/5/2014 14:26, Mgr. et Mgr. Jana Ilgová, Ph.D.

Abstract

V originále

Investigating the functions of various bioactive molecules produced by parasites expands our understanding of the principles of host-parasite interaction. Several of these compounds were proven to play a key role in a manipulation of the host immune system and thus maximizing the parasite success; among them also the cysteine peptidase inhibitors (CPI) were recorded. Our work is focused on characterization of the properties of CPI expressed by four helminth model species representing four big taxonomic groups – Monogenea, Cestoda, Trematoda and Nematoda. Prior to the functional analysis we searched the transcriptomic and/or genomic data of Eudiplozoon nipponicum, Hymenolepis diminuta, Fascioloides magna and Trichinella spiralis for the presence of conserved cystatin domains. We identified distinct families of CPI (stefins, cystatins and kininogens) and observed differences among the studied species. We designed specific/degenerate primers; amplified, cloned and sequenced the selected genes. The recombinant forms of particular CPI will be prepared and characterized in detail to reveal their function.

Links

GAP506/12/1258, research and development project
Name: Interakce hostitel-parazit u krevsajících diplozoidních monogeneí: Výzkum vysoce specializovaných adaptací k parazitismu
Investor: Czech Science Foundation
GBP505/12/G112, research and development project
Name: ECIP - Evropské centrum ichtyoparazitologie
Investor: Czech Science Foundation