Coilin is rapidly recruited to UVA-induced DNA lesions and
gamma-radiation affects localized movement of Cajal bodies

J 2014

Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies

BÁRTOVÁ, Eva; Veronika FOLTÁNKOVÁ; Soňa LEGARTOVÁ; Petra SEHNALOVÁ; Dmitry SOROKIN et al.

Základní údaje

Originální název

Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies

Autoři

BÁRTOVÁ, Eva; Veronika FOLTÁNKOVÁ; Soňa LEGARTOVÁ ; Petra SEHNALOVÁ; Dmitry SOROKIN; Jana SUCHÁNKOVÁ a Stanislav KOZUBEK

Vydání

Nucleus, Landes Bioscience, 2014, 1949-1034

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.033

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14330/14:00073626

Organizační jednotka

Fakulta informatiky

Klíčová slova anglicky

DNA repair; chromatin; nucleus; nucleolus; Cajal bodies; coilin

Štítky

CBIA, cbia-web

Změněno: 27. 4. 2015 03:55, RNDr. Pavel Šmerk, Ph.D.

Anotace

V originále

Cajal bodies are important nuclear structures containing proteins that preferentially regulate RNA-related metabolism. We investigated the cell-type specific nuclear distribution of Cajal bodies and the level of coilin, a protein of Cajal bodies, in non-irradiated and irradiated human tumor cell lines and embryonic stem (ES) cells. Cajal bodies were localized in different nuclear compartments, including DAPI-poor regions, in the proximity of chromocenters, and adjacent to nucleoli. The number of Cajal bodies per nucleus was cell cycle-dependent, with higher numbers occurring during G2 phase. Human ES cells contained a high coilin level in the nucleoplasm, but coilin-positive Cajal bodies were also identified in nuclei of mouse and human ES cells. Coilin, but not SMN, recognized UVA-induced DNA lesions, which was cell cycle-independent. Treatment with gamma-radiation reduced the localized movement of Cajal bodies in many cell types and GFP-coilin fluorescence recovery after photobleaching was very fast in nucleoplasm in comparison with GFP-coilin recovery in DNA lesions. By contrast, nucleolus-localized coilin displayed very slow fluorescence recovery after photobleaching, which indicates very slow rates of protein diffusion, especially in mouse ES cell nucleoli.

Návaznosti

CZ.1.07/2.3.00/30.0030, interní kód MU

Název: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Rozvoj lidských zdrojů pro oblast buněčné biologie, 2.3 Lidské zdroje ve výzkumu a vývoji

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

BÁRTOVÁ, Eva; Veronika FOLTÁNKOVÁ; Soňa LEGARTOVÁ; Petra SEHNALOVÁ; Dmitry SOROKIN; Jana SUCHÁNKOVÁ a Stanislav KOZUBEK. Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies. Nucleus. Landes Bioscience, 2014, roč. 5, č. 3, s. 269-277. ISSN 1949-1034. Dostupné z: https://doi.org/10.4161/nucl.29229.

@article{1182653,
   author = {Bártová, Eva and Foltánková, Veronika and Legartová, Soňa and Sehnalová, Petra and Sorokin, Dmitry and Suchánková, Jana and Kozubek, Stanislav},
   article_number = {3},
   doi = {https://doi.org/10.4161/nucl.29229},
   keywords = {DNA repair; chromatin; nucleus; nucleolus; Cajal bodies; coilin},
   language = {eng},
   issn = {1949-1034},
   journal = {Nucleus},
   title = {Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies},
   volume = {5},
   year = {2014}
}

TY  - JOUR
ID  - 1182653
AU  - Bártová, Eva - Foltánková, Veronika - Legartová, Soňa - Sehnalová, Petra - Sorokin, Dmitry - Suchánková, Jana - Kozubek, Stanislav
PY  - 2014
TI  - Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies
JF  - Nucleus
VL  - 5
IS  - 3
SP  - 269-277
EP  - 269-277
PB  - Landes Bioscience
SN  - 19491034
KW  - DNA repair
KW  - chromatin
KW  - nucleus
KW  - nucleolus
KW  - Cajal bodies
KW  - coilin
N2  - Cajal bodies are important nuclear structures containing proteins that preferentially regulate RNA-related metabolism. We investigated the cell-type specific nuclear distribution of Cajal bodies and the level of coilin, a protein of Cajal bodies, in non-irradiated and irradiated human tumor cell lines and embryonic stem (ES) cells. Cajal bodies were localized in different nuclear compartments, including DAPI-poor regions, in the proximity of chromocenters, and adjacent to nucleoli. The number of Cajal bodies per nucleus was cell cycle-dependent, with higher numbers occurring during G2 phase. Human ES cells contained a high coilin level in the nucleoplasm, but coilin-positive Cajal bodies were also identified in nuclei of mouse and human ES cells. Coilin, but not SMN, recognized UVA-induced DNA lesions, which was cell cycle-independent. Treatment with gamma-radiation reduced the localized movement of Cajal bodies in many cell types and GFP-coilin fluorescence recovery after photobleaching was very fast in nucleoplasm in comparison with GFP-coilin recovery in DNA lesions. By contrast, nucleolus-localized coilin displayed very slow fluorescence recovery after photobleaching, which indicates very slow rates of protein diffusion, especially in mouse ES cell nucleoli.
ER  -

BÁRTOVÁ, Eva; Veronika FOLTÁNKOVÁ; Soňa LEGARTOVÁ; Petra SEHNALOVÁ; Dmitry SOROKIN; Jana SUCHÁNKOVÁ a Stanislav KOZUBEK. Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies. \textit{Nucleus}. Landes Bioscience, 2014, roč.~5, č.~3, s.~269-277. ISSN~1949-1034. Dostupné z: https://doi.org/10.4161/nucl.29229.

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