Bioluminescent assay for evaluating antimicrobial activity in
insect haemolymph

J 2014

Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph

VOJTEK, Libor; Pavel DOBEŠ; Ender BÜYÜKGÜZEL; Janne ATOSUO; Pavel HYRŠL et al.

Základní údaje

Originální název

Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph

Autoři

VOJTEK, Libor; Pavel DOBEŠ; Ender BÜYÜKGÜZEL; Janne ATOSUO a Pavel HYRŠL

Vydání

European Journal of Entomology, 2014, 1210-5759

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.975

Kód RIV

RIV/00216224:14310/14:00073253

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.14411/eje.2014.045

UT WoS

000343664400004

EID Scopus

2-s2.0-84904608455

Klíčová slova česky

Antibacterial activity; antimicrobial peptides; Lepidoptera; bioluminescent bacteria; Bombyx mori; Escherichia coli; Galleria mellonella; Photorhabdus luminescens

Klíčová slova anglicky

Antibacterial activity; antimicrobial peptides; Lepidoptera; bioluminescent bacteria; Bombyx mori; Escherichia coli; Galleria mellonella; Photorhabdus luminescens

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 5. 2015 13:42, Mgr. Pavel Dobeš, Ph.D.

Anotace

V originále

We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria, Photorhabdus luminescens and transformed Escherichia coli, which can be used as a real-time measurement of antibacterial activity in insect haemolymph. This method is based on the production of the bioluminescence signal depending on the viability of bacterial cells. We observed a significant rapid dose-dependent decrease in bioluminescence using both bacterial species, and Bombyx mori or Galleria mellonella haemolymph, which was confirmed by the decrease in bacterial viability determined by plating. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed for haemolymph plasma without haemocytes. Antibacterial activity directed against Gram negative bacteria was recorded in unaffected insect larvae as well as after septic injury; increased antibacterial activity of haemolymph was detected in the latter case confirming the inducibility of antimicrobial agents. We think it is likely that this method could be widely used for determining antibacterial activity in insects and other invertebrates.

Návaznosti

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GP206/09/P470, projekt VaV

Název: Vliv inhibitorů biosyntézy eikosanoidů na imunitu zavíječe voskového Galleria mellonella

Investor: Grantová agentura ČR, Vliv inhibitorů biosyntézy eikonsanoidů na imunitu zavíječe voskového Galleria mellonella

QJ1210047, projekt VaV

Název: Vývoj nových prostředků pro podporu imunity včel, prevenci a léčbu včelích onemocnění (Akronym: Probiotika - imunita včel)

Investor: Ministerstvo zemědělství ČR, Vývoj nových prostředků pro podporu imunity včel, prevenci a léčbu včelích onemocnění

Citovat

VOJTEK, Libor; Pavel DOBEŠ; Ender BÜYÜKGÜZEL; Janne ATOSUO a Pavel HYRŠL. Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph. European Journal of Entomology. 2014, roč. 111, č. 3, s. 335-340. ISSN 1210-5759. Dostupné z: https://doi.org/10.14411/eje.2014.045.

@article{1183817,
   author = {Vojtek, Libor and Dobeš, Pavel and Büyükgüzel, Ender and Atosuo, Janne and Hyršl, Pavel},
   article_number = {3},
   doi = {https://doi.org/10.14411/eje.2014.045},
   keywords = {Antibacterial activity; antimicrobial peptides; Lepidoptera; bioluminescent bacteria; Bombyx mori; Escherichia coli; Galleria mellonella; Photorhabdus luminescens},
   language = {eng},
   issn = {1210-5759},
   journal = {European Journal of Entomology},
   title = {Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph},
   volume = {111},
   year = {2014}
}

TY  - JOUR
ID  - 1183817
AU  - Vojtek, Libor - Dobeš, Pavel - Büyükgüzel, Ender - Atosuo, Janne - Hyršl, Pavel
PY  - 2014
TI  - Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph
JF  - European Journal of Entomology
VL  - 111
IS  - 3
SP  - 335-340
EP  - 335-340
SN  - 12105759
KW  - Antibacterial activity
KW  - antimicrobial peptides
KW  - Lepidoptera
KW  - bioluminescent bacteria
KW  - Bombyx mori
KW  - Escherichia coli
KW  - Galleria mellonella
KW  - Photorhabdus luminescens
N2  - We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria, Photorhabdus luminescens and transformed Escherichia coli, which can be used as a real-time measurement of antibacterial activity in insect haemolymph. This method is based on the production of the bioluminescence signal depending on the viability of bacterial cells. We observed a significant rapid dose-dependent decrease in bioluminescence using both bacterial species, and Bombyx mori or Galleria mellonella haemolymph, which was confirmed by the decrease in bacterial viability determined by plating. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed for haemolymph plasma without haemocytes. Antibacterial activity directed against Gram negative bacteria was recorded in unaffected insect larvae as well as after septic injury; increased antibacterial activity of haemolymph was detected in the latter case confirming the inducibility of antimicrobial agents. We think it is likely that this method could be widely used for determining antibacterial activity in insects and other invertebrates.
ER  -

VOJTEK, Libor; Pavel DOBEŠ; Ender BÜYÜKGÜZEL; Janne ATOSUO a Pavel HYRŠL. Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph. \textit{European Journal of Entomology}. 2014, roč.~111, č.~3, s.~335-340. ISSN~1210-5759. Dostupné z: https://doi.org/10.14411/eje.2014.045.

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