Study of metallothionein-quantum dots interactions

J 2014

Study of metallothionein-quantum dots interactions

TMEJOVA, Katerina; David HYNEK; Pavel KOPEL; Sona KRIZKOVA; Iva BLAZKOVA et al.

Základní údaje

Originální název

Study of metallothionein-quantum dots interactions

Autoři

TMEJOVA, Katerina; David HYNEK; Pavel KOPEL; Sona KRIZKOVA; Iva BLAZKOVA; Libuše TRNKOVÁ; Vojtěch ADAM a René KIZEK

Vydání

Colloids and Surfaces B: Biointerferences, Amsterdam, Elsevier, 2014, 0927-7765

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.152

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/14:00075727

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Electrochemical detection; Metallothionein; Quantum dots; Interaction

Štítky

AKR, rivok

Změněno: 23. 4. 2020 17:51, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Nanoparticles have gained increasing interest in medical and in vivo applications. Metallothionein (MT) is well known as a maintainer of metal ions balance in intracellular space. This is due to high affinity of this protein to any reactive species including metals and reactive oxygen species. The purpose of this study was to determine the metallothionein-quantum dots interactions that were investigated by spectral and electrochemical techniques. CuS, CdS, PbS, and CdTe quantum dots (QDs) were analysed. The highest intensity was shown for CdTe, than for CdS measured by fluorescence. These results were supported by statistical analysis and considered as significant. Further, these interactions were analysed using gel electrophoresis, where MT aggregates forming after interactions with QDs were detected. Using differential pulse voltammetry Brdicka reaction, QDs and MT were studied. This method allowed us to confirm spectral results and, moreover, to observe the changes in MT structure causing new voltammetric peaks called X and Y, which enhanced with the prolonged time of interaction up to 6 h. (C) 2014 Elsevier B.V. All rights reserved.

Návaznosti

CZ.1.05/1.1.00/02.0068, interní kód MU

Název: CEITEC - středoevropský technologický institut (Akronym: CEITEC)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC - středoevropský technologický institut, 1.1 Evropská centra excelence

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

TMEJOVA, Katerina; David HYNEK; Pavel KOPEL; Sona KRIZKOVA; Iva BLAZKOVA; Libuše TRNKOVÁ; Vojtěch ADAM a René KIZEK. Study of metallothionein-quantum dots interactions. Colloids and Surfaces B: Biointerferences. Amsterdam: Elsevier, 2014, roč. 117, May, s. 534-537. ISSN 0927-7765. Dostupné z: https://doi.org/10.1016/j.colsurfb.2014.03.013.

@article{1186255,
   author = {Tmejova, Katerina and Hynek, David and Kopel, Pavel and Krizkova, Sona and Blazkova, Iva and Trnková, Libuše and Adam, Vojtěch and Kizek, René},
   article_location = {Amsterdam},
   article_number = {May},
   doi = {https://doi.org/10.1016/j.colsurfb.2014.03.013},
   keywords = {Electrochemical detection; Metallothionein; Quantum dots; Interaction},
   language = {eng},
   issn = {0927-7765},
   journal = {Colloids and Surfaces B: Biointerferences},
   title = {Study of metallothionein-quantum dots interactions},
   url = {http://dx.doi.org/10.1016/j.colsurfb.2014.03.013},
   volume = {117},
   year = {2014}
}

TY  - JOUR
ID  - 1186255
AU  - Tmejova, Katerina - Hynek, David - Kopel, Pavel - Krizkova, Sona - Blazkova, Iva - Trnková, Libuše - Adam, Vojtěch - Kizek, René
PY  - 2014
TI  - Study of metallothionein-quantum dots interactions
JF  - Colloids and Surfaces B: Biointerferences
VL  - 117
IS  - May
SP  - 534-537
EP  - 534-537
PB  - Elsevier
SN  - 09277765
KW  - Electrochemical detection
KW  - Metallothionein
KW  - Quantum dots
KW  - Interaction
UR  - http://dx.doi.org/10.1016/j.colsurfb.2014.03.013
L2  - http://dx.doi.org/10.1016/j.colsurfb.2014.03.013
N2  - Nanoparticles have gained increasing interest in medical and in vivo applications. Metallothionein (MT) is well known as a maintainer of metal ions balance in intracellular space. This is due to high affinity of this protein to any reactive species including metals and reactive oxygen species. The purpose of this study was to determine the metallothionein-quantum dots interactions that were investigated by spectral and electrochemical techniques. CuS, CdS, PbS, and CdTe quantum dots (QDs) were analysed. The highest intensity was shown for CdTe, than for CdS measured by fluorescence. These results were supported by statistical analysis and considered as significant. Further, these interactions were analysed using gel electrophoresis, where MT aggregates forming after interactions with QDs were detected. Using differential pulse voltammetry Brdicka reaction, QDs and MT were studied. This method allowed us to confirm spectral results and, moreover, to observe the changes in MT structure causing new voltammetric peaks called X and Y, which enhanced with the prolonged time of interaction up to 6 h. (C) 2014 Elsevier B.V. All rights reserved.
ER  -

TMEJOVA, Katerina; David HYNEK; Pavel KOPEL; Sona KRIZKOVA; Iva BLAZKOVA; Libuše TRNKOVÁ; Vojtěch ADAM a René KIZEK. Study of metallothionein-quantum dots interactions. \textit{Colloids and Surfaces B: Biointerferences}. Amsterdam: Elsevier, 2014, roč.~117, May, s.~534-537. ISSN~0927-7765. Dostupné z: https://doi.org/10.1016/j.colsurfb.2014.03.013.

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