Cell differentiation along multiple pathways accompanied by
changes in histone acetylation status

J 2014

Cell differentiation along multiple pathways accompanied by changes in histone acetylation status

LEGARTOVÁ, Soňa; Stanislav KOZUBEK; Michal FRÁNEK; Zbyněk ZDRÁHAL; Gabriela LOCHMANOVÁ et. al.

Basic information

Original name

Cell differentiation along multiple pathways accompanied by changes in histone acetylation status

Authors

LEGARTOVÁ, Soňa (203 Czech Republic); Stanislav KOZUBEK (203 Czech Republic); Michal FRÁNEK (203 Czech Republic); Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution); Gabriela LOCHMANOVÁ (203 Czech Republic, belonging to the institution); Nadine MARTINET (250 France) and Eva BÁRTOVÁ (203 Czech Republic)

Edition

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, OTTAWA, CANADIAN SCIENCE PUBLISHING, 2014, 0829-8211

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

Genetics and molecular biology

Country of publisher

Canada

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 2.152

RIV identification code

RIV/00216224:14740/14:00073697

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1139/bcb-2013-0082

UT WoS

000334029200001

EID Scopus

2-s2.0-84898061760

Keywords in English

NEWLY SYNTHESIZED HISTONES; EMBRYONIC STEM-CELLS; NUCLEAR ARRANGEMENT; REPLICATION FORK; H3K9 ACETYLATION; CHROMATIN; TRANSCRIPTION; H4; COMPLEX; SITES

Abstract

In the original language

Post-translational modification of histones is fundamental to the regulation of basic nuclear processes and subsequent cellular events, including differentiation. In this study, we analyzed acetylated forms of histones H2A, H2B, and H4 during induced differentiation in mouse (mESCs) and human (hESCs) embryonic stem cells and during induced enterocytic differentiation of colon cancer cells in vitro. Endoderm-like differentiation of mESCs induced by retinoic acid and enterocytic differentiation induced by histone deacetylase inhibitor sodium butyrate were accompanied by increased mono-, di-, and tri-acetylation of histone H2B and a pronounced increase in di- and tri-acetylation of histone H4. In enterocytes, mono- acetylation of histone H2A also increased and tetra-acetylation of histone H4 appeared only after induction of this differentiation pathway. During differentiation of hESCs, we observed increased mono-acetylation and decreased tri-acetylation of H2B. Mono-, di-, and tri-acetylation of H4 were reduced, manifested by a significant increase in nonacetylated H4 histones. Levels of acetylated histones increased during induced differentiation in mESCs and during histone deacetylase (HDAC) inhibitor-induced enterocytic differentiation, whereas differentiation of human ESCs was associated with reduced acetylation of histones H2B and H4.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GBP302/12/G157, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

Cite

LEGARTOVÁ, Soňa; Stanislav KOZUBEK; Michal FRÁNEK; Zbyněk ZDRÁHAL; Gabriela LOCHMANOVÁ; Nadine MARTINET and Eva BÁRTOVÁ. Cell differentiation along multiple pathways accompanied by changes in histone acetylation status. BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE. OTTAWA: CANADIAN SCIENCE PUBLISHING, 2014, vol. 92, No 2, p. 85-93. ISSN 0829-8211. Available from: https://dx.doi.org/10.1139/bcb-2013-0082.

@article{1186358,
   author = {Legartová, Soňa and Kozubek, Stanislav and Fránek, Michal and Zdráhal, Zbyněk and Lochmanová, Gabriela and Martinet, Nadine and Bártová, Eva},
   article_location = {OTTAWA},
   article_number = {2},
   doi = {http://dx.doi.org/10.1139/bcb-2013-0082},
   keywords = {NEWLY SYNTHESIZED HISTONES; EMBRYONIC STEM-CELLS; NUCLEAR ARRANGEMENT; REPLICATION FORK; H3K9 ACETYLATION; CHROMATIN; TRANSCRIPTION; H4; COMPLEX; SITES},
   language = {eng},
   issn = {0829-8211},
   journal = {BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE},
   title = {Cell differentiation along multiple pathways accompanied by changes in histone acetylation status},
   url = {http://www.nrcresearchpress.com/doi/abs/10.1139/bcb-2013-0082?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed&#.VQAanuFFrs4},
   volume = {92},
   year = {2014}
}

TY  - JOUR
ID  - 1186358
AU  - Legartová, Soňa - Kozubek, Stanislav - Fránek, Michal - Zdráhal, Zbyněk - Lochmanová, Gabriela - Martinet, Nadine - Bártová, Eva
PY  - 2014
TI  - Cell differentiation along multiple pathways accompanied by changes in histone acetylation status
JF  - BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
VL  - 92
IS  - 2
SP  - 85-93
EP  - 85-93
PB  - CANADIAN SCIENCE PUBLISHING
SN  - 08298211
KW  - NEWLY SYNTHESIZED HISTONES
KW  - EMBRYONIC STEM-CELLS
KW  - NUCLEAR ARRANGEMENT
KW  - REPLICATION FORK
KW  - H3K9 ACETYLATION
KW  - CHROMATIN
KW  - TRANSCRIPTION
KW  - H4
KW  - COMPLEX
KW  - SITES
UR  - http://www.nrcresearchpress.com/doi/abs/10.1139/bcb-2013-0082?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed&#.VQAanuFFrs4
L2  - http://www.nrcresearchpress.com/doi/abs/10.1139/bcb-2013-0082?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed&#.VQAanuFFrs4
N2  - Post-translational modification of histones is fundamental to the regulation of basic nuclear processes and subsequent cellular events, including differentiation. In this study, we analyzed acetylated forms of histones H2A, H2B, and H4 during induced differentiation in mouse (mESCs) and human (hESCs) embryonic stem cells and during induced enterocytic differentiation of colon cancer cells in vitro. Endoderm-like differentiation of mESCs induced by retinoic acid and enterocytic differentiation induced by histone deacetylase inhibitor sodium butyrate were accompanied by increased mono-, di-, and tri-acetylation of histone H2B and a pronounced increase in di- and tri-acetylation of histone H4. In enterocytes, mono- acetylation of histone H2A also increased and tetra-acetylation of histone H4 appeared only after induction of this differentiation pathway. During differentiation of hESCs, we observed increased mono-acetylation and decreased tri-acetylation of H2B. Mono-, di-, and tri-acetylation of H4 were reduced, manifested by a significant increase in nonacetylated H4 histones. Levels of acetylated histones increased during induced differentiation in mESCs and during histone deacetylase (HDAC) inhibitor-induced enterocytic differentiation, whereas differentiation of human ESCs was associated with reduced acetylation of histones H2B and H4.
ER  -

LEGARTOVÁ, Soňa; Stanislav KOZUBEK; Michal FRÁNEK; Zbyněk ZDRÁHAL; Gabriela LOCHMANOVÁ; Nadine MARTINET and Eva BÁRTOVÁ. Cell differentiation along multiple pathways accompanied by changes in histone acetylation status. \textit{BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE}. OTTAWA: CANADIAN SCIENCE PUBLISHING, 2014, vol.~92, No~2, p.~85-93. ISSN~0829-8211. Available from: https://dx.doi.org/10.1139/bcb-2013-0082.

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