Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline
on Metal Resistant and Non-Resistant Staphylococcus aureus

J 2014

Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus

CHUDOBOVA, Dagmar, Simona DOSTALOVA, Iva BLAZKOVA, Petr MICHALEK, Branislav RUTTKAY-NEDECKY et. al.

Basic information

Original name

Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus

Authors

CHUDOBOVA, Dagmar (203 Czech Republic), Simona DOSTALOVA (203 Czech Republic), Iva BLAZKOVA (203 Czech Republic), Petr MICHALEK (203 Czech Republic), Branislav RUTTKAY-NEDECKY (203 Czech Republic), Matej SKLENAR (203 Czech Republic), Lukas NEJDL (203 Czech Republic), Jiri KUDR (203 Czech Republic), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Kateřina TMEJOVÁ (203 Czech Republic), Marie KONECNA (203 Czech Republic), Marketa VACULOVICOVA (203 Czech Republic), Daniel HYNEK (203 Czech Republic), Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution), Jindrich KYNICKY (203 Czech Republic), Rene KIZEK (203 Czech Republic) and Vojtech ADAM (203 Czech Republic)

Edition

INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, BASEL, Molecular Diversity Preservation International, 2014, 1660-4601

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.063

RIV identification code

RIV/00216224:14110/14:00075837

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/ijerph110303233

UT WoS

000334438200056

Keywords in English

S. aureus; antimicrobial resistance; antibiotics; metal resistance; cross resistance; growth curves; inhibition concentrations; spectrophotometry

Abstract

V originále

There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects of antibiotics on Staphylococcus aureus non-resistant strain and strains resistant to cadmium or lead ions. Metal resistant strains were created by the gradual addition of 2 mM solution of metal ions (cadmium or lead) to the S. aureus culture. An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 mu M) on the resistant strains was observed using a method of growth curves. A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics. On the other hand, the addition of streptomycin and ampicillin did not inhibit the growth of lead resistant strain. Other antibiotics were still toxic to the bacterial cells. Significant differences in the morphology of cell walls were indicated by changes in the cell shape. Our data show that the presence of metal ions in the urban environment may contribute to the development of bacterial strain resistance to other substances including antibiotics, which would have an impact on public health.

Citovat

CHUDOBOVA, Dagmar, Simona DOSTALOVA, Iva BLAZKOVA, Petr MICHALEK, Branislav RUTTKAY-NEDECKY, Matej SKLENAR, Lukas NEJDL, Jiri KUDR, Jaromír GUMULEC, Kateřina TMEJOVÁ, Marie KONECNA, Marketa VACULOVICOVA, Daniel HYNEK, Michal MASAŘÍK, Jindrich KYNICKY, Rene KIZEK and Vojtech ADAM. Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus. INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH. BASEL: Molecular Diversity Preservation International, 2014, vol. 11, No 3, p. 3233-3255. ISSN 1660-4601. Available from: https://dx.doi.org/10.3390/ijerph110303233.

@article{1187235,
   author = {Chudobova, Dagmar and Dostalova, Simona and Blazkova, Iva and Michalek, Petr and RuttkayandNedecky, Branislav and Sklenar, Matej and Nejdl, Lukas and Kudr, Jiri and Gumulec, Jaromír and Tmejová, Kateřina and Konecna, Marie and Vaculovicova, Marketa and Hynek, Daniel and Masařík, Michal and Kynicky, Jindrich and Kizek, Rene and Adam, Vojtech},
   article_location = {BASEL},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/ijerph110303233},
   keywords = {S. aureus; antimicrobial resistance; antibiotics; metal resistance; cross resistance; growth curves; inhibition concentrations; spectrophotometry},
   language = {eng},
   issn = {1660-4601},
   journal = {INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH},
   title = {Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus},
   volume = {11},
   year = {2014}
}

TY  - JOUR
ID  - 1187235
AU  - Chudobova, Dagmar - Dostalova, Simona - Blazkova, Iva - Michalek, Petr - Ruttkay-Nedecky, Branislav - Sklenar, Matej - Nejdl, Lukas - Kudr, Jiri - Gumulec, Jaromír - Tmejová, Kateřina - Konecna, Marie - Vaculovicova, Marketa - Hynek, Daniel - Masařík, Michal - Kynicky, Jindrich - Kizek, Rene - Adam, Vojtech
PY  - 2014
TI  - Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus
JF  - INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
VL  - 11
IS  - 3
SP  - 3233-3255
EP  - 3233-3255
PB  - Molecular Diversity Preservation International
SN  - 16604601
KW  - S. aureus
KW  - antimicrobial resistance
KW  - antibiotics
KW  - metal resistance
KW  - cross resistance
KW  - growth curves
KW  - inhibition concentrations
KW  - spectrophotometry
N2  - There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects of antibiotics on Staphylococcus aureus non-resistant strain and strains resistant to cadmium or lead ions. Metal resistant strains were created by the gradual addition of 2 mM solution of metal ions (cadmium or lead) to the S. aureus culture. An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 mu M) on the resistant strains was observed using a method of growth curves. A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics. On the other hand, the addition of streptomycin and ampicillin did not inhibit the growth of lead resistant strain. Other antibiotics were still toxic to the bacterial cells. Significant differences in the morphology of cell walls were indicated by changes in the cell shape. Our data show that the presence of metal ions in the urban environment may contribute to the development of bacterial strain resistance to other substances including antibiotics, which would have an impact on public health.
ER  -

CHUDOBOVA, Dagmar, Simona DOSTALOVA, Iva BLAZKOVA, Petr MICHALEK, Branislav RUTTKAY-NEDECKY, Matej SKLENAR, Lukas NEJDL, Jiri KUDR, Jaromír GUMULEC, Kateřina TMEJOVÁ, Marie KONECNA, Marketa VACULOVICOVA, Daniel HYNEK, Michal MASAŘÍK, Jindrich KYNICKY, Rene KIZEK and Vojtech ADAM. Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus. \textit{INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH}. BASEL: Molecular Diversity Preservation International, 2014, vol.~11, No~3, p.~3233-3255. ISSN~1660-4601. Available from: https://dx.doi.org/10.3390/ijerph110303233.

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