Paclitaxel isomerisation in polymeric micelles based on
hydrophobized hyaluronic acid

J 2014

Paclitaxel isomerisation in polymeric micelles based on hydrophobized hyaluronic acid

SMEJKALOVA, D; Kristina NEŠPOROVÁ; M HERMANNOVA; G HUERTA-ANGELES; D COZIKOVA et al.

Základní údaje

Originální název

Paclitaxel isomerisation in polymeric micelles based on hydrophobized hyaluronic acid

Autoři

SMEJKALOVA, D; Kristina NEŠPOROVÁ; M HERMANNOVA; G HUERTA-ANGELES; D COZIKOVA; Lucie VIŠTEJNOVÁ; B SAFRANKOVA; J NOVOTNY; Jindra KUČEŘÍKOVÁ a V VELEBNY

Vydání

International Journal of Pharmaceutics, AMSTERDAM, ELSEVIER SCIENCE BV, 2014, 0378-5173

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.650

Označené pro přenos do RIV

DOI

https://doi.org/10.1016/j.ijpharm.2014.03.024

UT WoS

000335441600018

Klíčová slova anglicky

Polymeric micelle; Hyaluronan; Paclitaxel; Stability; Isomer

Změněno: 4. 7. 2014 16:20, Mgr. Kristina Nešporová, Ph.D.

Anotace

V originále

Physical and chemical structure of paclitaxel (PTX) was studied after its incorporation into polymeric micelles made of hyaluronic acid (HA) (M-w = 15 kDa) grafted with C6 or C18:1 acyl chains. PTX was physically incorporated into the micellar core by solvent evaporation technique. Maximum loading capacity for HAC6 and HAC18:1 was determined to be 2 and 14 wt.%, respectively. The loading efficiency was higher for HAC18:1 and reached 70%. Independently of the derivative, loaded HA micelles had spherical size of approximately 60-80 nm and demonstrated slow and sustained release of PTX in vitro. PTX largely changed its form from crystalline to amorphous after its incorporation into the micelle's interior. This transformation increased PTX sensitivity towards stressing conditions, mainly to UV light exposure, during which the structure of amorphous PTX isomerized and formed C3-C11 bond within its structure. In vitro cytotoxicity assay revealed that polymeric micelles loaded with PTX isomer had higher cytotoxic effect to normal human dermal fibroblasts (NHDF) and human colon carcinoma cells (HCT-116) than the same micelles loaded with non-isomerized PTX. Further observation indicated that PTX isomer influenced in different ways cell morphology and markers of cell cycle. Taken together, PTX isomer loaded in nanocarrier systems may have improved anticancer activity in vivo than pure PTX. (C) 2014 Elsevier B.V. All rights reserved.

Citovat

SMEJKALOVA, D; Kristina NEŠPOROVÁ; M HERMANNOVA; G HUERTA-ANGELES; D COZIKOVA; Lucie VIŠTEJNOVÁ; B SAFRANKOVA; J NOVOTNY; Jindra KUČEŘÍKOVÁ a V VELEBNY. Paclitaxel isomerisation in polymeric micelles based on hydrophobized hyaluronic acid. International Journal of Pharmaceutics. AMSTERDAM: ELSEVIER SCIENCE BV, 2014, roč. 466, 1-2, s. 147-155. ISSN 0378-5173. Dostupné z: https://doi.org/10.1016/j.ijpharm.2014.03.024.

@article{1188186,
   author = {Smejkalova, D and Nešporová, Kristina and Hermannova, M and HuertaandAngeles, G and Cozikova, D and Vištejnová, Lucie and Safrankova, B and Novotny, J and Kučeříková, Jindra and Velebny, V},
   article_location = {AMSTERDAM},
   article_number = {1-2},
   doi = {https://doi.org/10.1016/j.ijpharm.2014.03.024},
   keywords = {Polymeric micelle; Hyaluronan; Paclitaxel; Stability; Isomer},
   language = {eng},
   issn = {0378-5173},
   journal = {International Journal of Pharmaceutics},
   title = {Paclitaxel isomerisation in polymeric micelles based on hydrophobized hyaluronic acid},
   volume = {466},
   year = {2014}
}

TY  - JOUR
ID  - 1188186
AU  - Smejkalova, D - Nešporová, Kristina - Hermannova, M - Huerta-Angeles, G - Cozikova, D - Vištejnová, Lucie - Safrankova, B - Novotny, J - Kučeříková, Jindra - Velebny, V
PY  - 2014
TI  - Paclitaxel isomerisation in polymeric micelles based on hydrophobized hyaluronic acid
JF  - International Journal of Pharmaceutics
VL  - 466
IS  - 1-2
SP  - 147-155
EP  - 147-155
PB  - ELSEVIER SCIENCE BV
SN  - 03785173
KW  - Polymeric micelle
KW  - Hyaluronan
KW  - Paclitaxel
KW  - Stability
KW  - Isomer
N2  - Physical and chemical structure of paclitaxel (PTX) was studied after its incorporation into polymeric micelles made of hyaluronic acid (HA) (M-w = 15 kDa) grafted with C6 or C18:1 acyl chains. PTX was physically incorporated into the micellar core by solvent evaporation technique. Maximum loading capacity for HAC6 and HAC18:1 was determined to be 2 and 14 wt.%, respectively. The loading efficiency was higher for HAC18:1 and reached 70%. Independently of the derivative, loaded HA micelles had spherical size of approximately 60-80 nm and demonstrated slow and sustained release of PTX in vitro. PTX largely changed its form from crystalline to amorphous after its incorporation into the micelle's interior. This transformation increased PTX sensitivity towards stressing conditions, mainly to UV light exposure, during which the structure of amorphous PTX isomerized and formed C3-C11 bond within its structure. In vitro cytotoxicity assay revealed that polymeric micelles loaded with PTX isomer had higher cytotoxic effect to normal human dermal fibroblasts (NHDF) and human colon carcinoma cells (HCT-116) than the same micelles loaded with non-isomerized PTX. Further observation indicated that PTX isomer influenced in different ways cell morphology and markers of cell cycle. Taken together, PTX isomer loaded in nanocarrier systems may have improved anticancer activity in vivo than pure PTX. (C) 2014 Elsevier B.V. All rights reserved.
ER  -

SMEJKALOVA, D; Kristina NEŠPOROVÁ; M HERMANNOVA; G HUERTA-ANGELES; D COZIKOVA; Lucie VIŠTEJNOVÁ; B SAFRANKOVA; J NOVOTNY; Jindra KUČEŘÍKOVÁ a V VELEBNY. Paclitaxel isomerisation in polymeric micelles based on hydrophobized hyaluronic acid. \textit{International Journal of Pharmaceutics}. AMSTERDAM: ELSEVIER SCIENCE BV, 2014, roč.~466, 1-2, s.~147-155. ISSN~0378-5173. Dostupné z: https://doi.org/10.1016/j.ijpharm.2014.03.024.

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