The role of HIF-1α in the regulation of cardiomyogenesis in
vitro

a 2014

The role of HIF-1α in the regulation of cardiomyogenesis in vitro

KUDOVÁ, Jana; Jiřina PROCHÁZKOVÁ; Ondřej VAŠÍČEK; Lucia BINÓ; Hana KOLÁŘOVÁ et al.

Základní údaje

Originální název

The role of HIF-1α in the regulation of cardiomyogenesis in vitro

Autoři

KUDOVÁ, Jana; Jiřina PROCHÁZKOVÁ; Ondřej VAŠÍČEK; Lucia BINÓ; Hana KOLÁŘOVÁ; Dominika SÝKOROVÁ; Pavel KARAS; Jiří PACHERNÍK a Lukáš KUBALA

Vydání

XXXIV Annual Meeting of the North American Section, Miami Beach, Florida, USA, 2014

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 8. 2014 14:52, Mgr. Ondřej Vašíček, Ph.D.

Anotace

V originále

A cardiac cell formation (cardiomyogenesis) is critically dependent on cell microenvironment. Oxygen availability is one of the most important factors which plays an essential role in cardiomyocytes differentiation. It is known that hypoxia (a low oxygen level) modulates the differentiation of murine embryonic stem cells to cardiomyocytes in vitro. Hypoxic conditions lead to the stabilization of hypoxia-inducible factor-1 alpha (HIF-1alpha), the central mediator of cellular transcription responses to oxygen tension. However, the role of HIF-1alpha in the regulation of cardiomyocyte differentiation is not well understood. With the aim of better understanding the role of HIF-1alpha within cardiomyocyte development and progenitor cell reprogramming, cardiomyogenesis in wild type and HIF-1alpha depleted murine embryonic stem cells was analyzed in vitro. The level of expression of individual cardiospecific genes was higher in the HIF-1alpha deficient cell line compared to wild type cells in the early phase of differentiation (on Day 5). In contrast, in the late phase of cardiac cell development (on Day 15 and later), these markers were lower in HIF-1alpha deficient cells. This suggests a higher state of maturity in HIF-1alpha deficient cells at the beginning of cardiomyogenesis differentiation. At the same time, the profile of cardiospecific markers suggests that a lower number of progenitor cells enter the differentiation process. In conclusion, HIF-1alpha is important for induction of the development of cardiomyogenic progenitors and has a significant effect on the maturation of early cardiomyocytes in in vitro culture.

Citovat

KUDOVÁ, Jana; Jiřina PROCHÁZKOVÁ; Ondřej VAŠÍČEK; Lucia BINÓ; Hana KOLÁŘOVÁ; Dominika SÝKOROVÁ; Pavel KARAS; Jiří PACHERNÍK a Lukáš KUBALA. The role of HIF-1α in the regulation of cardiomyogenesis in vitro. In XXXIV Annual Meeting of the North American Section, Miami Beach, Florida, USA. 2014.

@proceedings{1193854,
   author = {Kudová, Jana and Procházková, Jiřina and Vašíček, Ondřej and Binó, Lucia and Kolářová, Hana and Sýkorová, Dominika and Karas, Pavel and Pacherník, Jiří and Kubala, Lukáš},
   booktitle = {XXXIV Annual Meeting of the North American Section, Miami Beach, Florida, USA},
   title = {The role of HIF-1α in the regulation of cardiomyogenesis in vitro},
   year = {2014}
}

TY  - CONF
ID  - 1193854
AU  - Kudová, Jana - Procházková, Jiřina - Vašíček, Ondřej - Binó, Lucia - Kolářová, Hana - Sýkorová, Dominika - Karas, Pavel - Pacherník, Jiří - Kubala, Lukáš
PY  - 2014
TI  - The role of HIF-1α in the regulation of cardiomyogenesis in vitro
N2  - A cardiac cell formation (cardiomyogenesis) is critically dependent on cell microenvironment. Oxygen availability is one of the most important factors which plays an essential role in cardiomyocytes differentiation. It is known that hypoxia (a low oxygen level) modulates the differentiation of murine embryonic stem cells to cardiomyocytes in vitro. Hypoxic conditions lead to the stabilization of hypoxia-inducible factor-1 alpha (HIF-1alpha), the central mediator of cellular transcription responses to oxygen tension. However, the role of HIF-1alpha in the regulation of cardiomyocyte differentiation is not well understood. With the aim of better understanding the role of HIF-1alpha within cardiomyocyte development and progenitor cell reprogramming, cardiomyogenesis in wild type and HIF-1alpha depleted murine embryonic stem cells was analyzed in vitro. The level of expression of individual cardiospecific genes was higher in the HIF-1alpha deficient cell line compared to wild type cells in the early phase of differentiation (on Day 5). In contrast, in the late phase of cardiac cell development (on Day 15 and later), these markers were lower in HIF-1alpha deficient cells. This suggests a higher state of maturity in HIF-1alpha deficient cells at the beginning of cardiomyogenesis differentiation. At the same time, the profile of cardiospecific markers suggests that a lower number of progenitor cells enter the differentiation process. In conclusion, HIF-1alpha is important for induction of the development of cardiomyogenic progenitors and has a significant effect on the maturation of early cardiomyocytes in in vitro culture.
ER  -

KUDOVÁ, Jana; Jiřina PROCHÁZKOVÁ; Ondřej VAŠÍČEK; Lucia BINÓ; Hana KOLÁŘOVÁ; Dominika SÝKOROVÁ; Pavel KARAS; Jiří PACHERNÍK a Lukáš KUBALA. The role of HIF-1α in the regulation of cardiomyogenesis in vitro. In \textit{XXXIV Annual Meeting of the North American Section, Miami Beach, Florida, USA}. 2014.

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