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@article{1195413,
author = {Grillová, Linda and Pětrošová, Helena and Mikalová, Lenka and Strnadel, Radim and Dastychová, Eliška and Kuklová, Ivana and Kojanová, Martina and Kreidlová, Miluše and Vaňousová, Daniela and Hercogová, Jana and Procházka, Přemysl and Zákoucká, Hana and Krchňáková, Alena and Vašků, Vladimír and Šmajs, David},
article_location = {Washington},
article_number = {10},
doi = {http://dx.doi.org/10.1128/JCM.01292-14},
keywords = {23S RIBOSOMAL-RNA; AZITHROMYCIN RESISTANCE; CLINICAL ISOLATE; PRIMARY SYPHILIS; SUBSP PALLIDUM; POINT MUTATION; SAN-FRANCISCO; UNITED-STATES; STRAINS; CHINA},
language = {eng},
issn = {0095-1137},
journal = {Journal of Clinical Microbiology},
title = {Molecular Typing of Treponema pallidum in the Czech Republic during 2011 to 2013: Increased Prevalence of Identified Genotypes and of Isolates with Macrolide Resistance},
volume = {52},
year = {2014}
}
TY - JOUR
ID - 1195413
AU - Grillová, Linda - Pětrošová, Helena - Mikalová, Lenka - Strnadel, Radim - Dastychová, Eliška - Kuklová, Ivana - Kojanová, Martina - Kreidlová, Miluše - Vaňousová, Daniela - Hercogová, Jana - Procházka, Přemysl - Zákoucká, Hana - Krchňáková, Alena - Vašků, Vladimír - Šmajs, David
PY - 2014
TI - Molecular Typing of Treponema pallidum in the Czech Republic during 2011 to 2013: Increased Prevalence of Identified Genotypes and of Isolates with Macrolide Resistance
JF - Journal of Clinical Microbiology
VL - 52
IS - 10
SP - 3693-3700
EP - 3693-3700
PB - American Society for Microbiology
SN - 00951137
KW - 23S RIBOSOMAL-RNA
KW - AZITHROMYCIN RESISTANCE
KW - CLINICAL ISOLATE
KW - PRIMARY SYPHILIS
KW - SUBSP PALLIDUM
KW - POINT MUTATION
KW - SAN-FRANCISCO
KW - UNITED-STATES
KW - STRAINS
KW - CHINA
N2 - From January 2011 to December 2013, a total of 262 samples, from 188 patients suspected of having syphilis were tested for the presence of treponemal DNA by PCR amplification of five chromosomal loci, including the polA (TP0105), tmpC (TP0319), TP0136, TP0548, and 23S rRNA genes. Altogether, 146 samples from 103 patients were PCR positive for treponemal DNA. A set of 81 samples from 62 PCR-positive patients were typeable, and among them, nine different genotypes were identified. Compared to a previous study in the Czech Republic during 2004 to 2010, the number of genotypes detected among syphilis patients in a particular year increased to six in both 2012 and 2013, although they were not the same six. The proportion of macrolide-resistant clinical isolates in this 3-year study was 66.7%.
ER -
GRILLOVÁ, Linda, Helena PĚTROŠOVÁ, Lenka MIKALOVÁ, Radim STRNADEL, Eliška DASTYCHOVÁ, Ivana KUKLOVÁ, Martina KOJANOVÁ, Miluše KREIDLOVÁ, Daniela VAŇOUSOVÁ, Jana HERCOGOVÁ, Přemysl PROCHÁZKA, Hana ZÁKOUCKÁ, Alena KRCHŇÁKOVÁ, Vladimír VAŠKŮ a David ŠMAJS. Molecular Typing of Treponema pallidum in the Czech Republic during 2011 to 2013: Increased Prevalence of Identified Genotypes and of Isolates with Macrolide Resistance. Online. \textit{Journal of Clinical Microbiology}. Washington: American Society for Microbiology, 2014, roč.~52, č.~10, s.~3693-3700. ISSN~0095-1137. Dostupné z: https://dx.doi.org/10.1128/JCM.01292-14. [citováno 2024-04-24]
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