Resistance of Antarctic lichens and freshwater algae from James
Ross Island to radiation, temperature, dehydration, UV-B and
salt stress assessed by photosynthetic parameters

a 2014

Resistance of Antarctic lichens and freshwater algae from James Ross Island to radiation, temperature, dehydration, UV-B and salt stress assessed by photosynthetic parameters

BARTÁK, Miloš; Josef HÁJEK; Peter VÁCZI; Kateřina SKÁCELOVÁ; Kateřina BALARINOVÁ et al.

Základní údaje

Originální název

Resistance of Antarctic lichens and freshwater algae from James Ross Island to radiation, temperature, dehydration, UV-B and salt stress assessed by photosynthetic parameters

Název česky

Odolnost antarktických lišejníků a sladkovodních řas z ostrova Jamese Rosse vůči záření, teplotě, vyschnutí, stresu UV radiací a zasolením ohodnocená pomocí fotosyntetických parametrů.

Autoři

BARTÁK, Miloš; Josef HÁJEK; Peter VÁCZI; Kateřina SKÁCELOVÁ; Kateřina BALARINOVÁ; Kateřina TRNKOVÁ a Jana HAZDROVÁ

Vydání

SCAR Open Science Conference (Auckland), 2014

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nový Zéland

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Scientific Committee on Antarctic Research

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/14:00076371

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

řasy; lišejníky; fluorescence chlorofylu; fotosyntéza

Klíčová slova anglicky

algae; lichens; chlorophyll fluorescence; photosynthesis

Změněno: 9. 9. 2014 15:55, prof. Ing. Miloš Barták, CSc.

Anotace

ORIG CZ

V originále

Lichen species Usnea antarctica, Usnea aurantiaco-atra, Xanthoria elegans, and algae Klebsormidium sp. and Zygnema sp. were investigated in our study by various biochemical and biophysical approaches in order to evaluate their resistance to particular environmental factors. The methods comprised oxymetric, fluorometric (fast (OJIP), and slow Chl fluorescence curves with quenching analysis), spectrophotometric methods (UV screens, photosynthetic pigments, glutathione). In lichens, short-term photoinhibition using (i) high, (ii) medium light doses were used. Involvement of photoprotective mechanisms was investigated. Chl fluorescence parameters, such FV/FM, yield of PSII, fast transient (OJIP) revealed that Usnea antarctica was less susceptible to photoinhibition than U. aurantiaco-atra. More pronounced reduction in Chla,b contents was found in U. aurantiaco-atra than U. antarctica. Total GSH showed an initial increase (first 30-40 min) followed by a decrease (60 min) and an increase during dark recovery. Full GSH recovery was not finished in U. aurantiaco-atra even after 5 h indicating lower capacity of photoprotective mechanisms. OJIPs decreased in both species, however, the recovery of their shape was faster and more apparent in U. antarctica than in U. aurantiaco-atra. For two algal species, simultaneous measurements of oxygen evolution rate (OER) and effective quantum (PSII yield) were done at different temperatures to gain photosynthetic light-response curves. In both species, OER and yield PSII were well related throughout a range of light (0-500 micromols m-2 s-1 of PAR. In a photobioreactor (PSI), repetitive photoinhibition (high light doses) was applied Klebsormidium sp. culture by simultaneous measurements of OER and PSII yield. The data revealed large resistance against photoinhibition, however, the courses of respiratory OER and potential PSII yield measured at dark changed with increasing number of photoinhibitory treatments. The effects of NaCl treatment on excitation energy transfer from LHCs to PSIIs in two Antarctic algae: Klebsormidium sp. and Zygnema sp. were investigated using Chl fluorescence transients (OJIPs). Short-term salt stress led to significant changes in the shape of OJIPs indicating altered functioning of PSII. With time of exposition to NaCl treatment, the fluorescence yield at the phases J, I and P declined considerably in both species. Acknowledgements: The authors thank CzechPolar infrastructure of laboratory facilities.

Česky

Odkazujeme na anglickou anotaci

Návaznosti

MSM 143100007, záměr

Název: Ekologie pobřežní antarktické vegetační oázy

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Ekologie pobřežní antarktické vegetační oázy

Citovat

BARTÁK, Miloš; Josef HÁJEK; Peter VÁCZI; Kateřina SKÁCELOVÁ; Kateřina BALARINOVÁ; Kateřina TRNKOVÁ a Jana HAZDROVÁ. Resistance of Antarctic lichens and freshwater algae from James Ross Island to radiation, temperature, dehydration, UV-B and salt stress assessed by photosynthetic parameters. In SCAR Open Science Conference (Auckland). 2014.

@proceedings{1197061,
   author = {Barták, Miloš and Hájek, Josef and Váczi, Peter and Skácelová, Kateřina and Balarinová, Kateřina and Trnková, Kateřina and Hazdrová, Jana},
   booktitle = {SCAR Open Science Conference (Auckland)},
   keywords = {algae; lichens; chlorophyll fluorescence; photosynthesis},
   language = {eng},
   title = {Resistance of Antarctic lichens and freshwater algae from James Ross Island to radiation, temperature, dehydration, UV-B and salt stress assessed by photosynthetic parameters},
   url = {http://www.scar.org},
   year = {2014}
}

TY  - CONF
ID  - 1197061
AU  - Barták, Miloš - Hájek, Josef - Váczi, Peter - Skácelová, Kateřina - Balarinová, Kateřina - Trnková, Kateřina - Hazdrová, Jana
PY  - 2014
TI  - Resistance of Antarctic lichens and freshwater algae from James Ross Island to radiation, temperature, dehydration, UV-B and salt stress assessed by photosynthetic parameters
KW  - algae
KW  - lichens
KW  - chlorophyll fluorescence
KW  - photosynthesis
UR  - http://www.scar.org
L2  - http://www.scar.org
N2  - Lichen species Usnea antarctica, Usnea aurantiaco-atra, Xanthoria elegans, and algae Klebsormidium sp. and Zygnema sp. were investigated in our study by various biochemical and biophysical approaches in order to evaluate their resistance to particular environmental factors. The methods comprised oxymetric, fluorometric (fast (OJIP), and slow Chl fluorescence curves with quenching analysis), spectrophotometric methods (UV screens, photosynthetic pigments, glutathione). In lichens, short-term photoinhibition using (i) high, (ii) medium light doses were used. Involvement of photoprotective mechanisms was investigated. Chl fluorescence parameters, such FV/FM, yield of PSII, fast transient (OJIP) revealed that Usnea antarctica was less susceptible to photoinhibition than U. aurantiaco-atra. More pronounced reduction in Chla,b contents was found in U. aurantiaco-atra than U. antarctica. Total GSH showed an initial increase (first 30-40 min) followed by a decrease (60 min) and an increase during dark recovery. Full GSH recovery was not finished in U. aurantiaco-atra even after 5 h indicating lower capacity of photoprotective mechanisms. OJIPs decreased in both species, however, the recovery of their shape was faster and more apparent in U. antarctica than in U. aurantiaco-atra. For two algal species, simultaneous measurements of oxygen evolution rate (OER) and effective quantum (PSII yield) were done at different temperatures to gain photosynthetic light-response curves. In both species, OER and yield PSII were well related throughout a range of light (0-500 micromols m-2 s-1 of PAR. In a photobioreactor (PSI), repetitive photoinhibition (high light doses) was applied Klebsormidium sp. culture by simultaneous measurements of OER and PSII yield. The data revealed large resistance against photoinhibition, however, the courses of respiratory OER and potential PSII yield measured at dark changed with increasing number of photoinhibitory treatments. The effects of NaCl treatment on excitation energy transfer from LHCs to PSIIs in two Antarctic algae: Klebsormidium sp. and Zygnema sp. were investigated using Chl fluorescence transients (OJIPs). Short-term salt stress led to significant changes in the shape of OJIPs indicating altered functioning of PSII. With time of exposition to NaCl treatment, the fluorescence yield at the phases J, I and P declined considerably in both species. Acknowledgements: The authors thank CzechPolar infrastructure of laboratory facilities.
ER  -

Přehled o publikaci