NONLETHAL SCREENING OF BAT-WING SKIN WITH THE USE OF
ULTRAVIOLET FLUORESCENCE TO DETECT LESIONS INDICATIVE OF
WHITE-NOSE SYNDROME

J 2014

NONLETHAL SCREENING OF BAT-WING SKIN WITH THE USE OF ULTRAVIOLET FLUORESCENCE TO DETECT LESIONS INDICATIVE OF WHITE-NOSE SYNDROME

TURNER, Gregory; Carol METEYER; Hazel BARTON; John GUMBS; DeeAnn REEDER et al.

Základní údaje

Originální název

NONLETHAL SCREENING OF BAT-WING SKIN WITH THE USE OF ULTRAVIOLET FLUORESCENCE TO DETECT LESIONS INDICATIVE OF WHITE-NOSE SYNDROME

Autoři

TURNER, Gregory; Carol METEYER; Hazel BARTON; John GUMBS; DeeAnn REEDER; Barrie OVERTON; Hana BANĎOUCHOVÁ; Tomáš BARTONIČKA; Natália MARTÍNKOVÁ; Jiří PIKULA; Jan ZUKAL a David BLEHERT

Vydání

Journal of Wildlife Diseases, Wildlife Disease Association, 2014, 0090-3558

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.355

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/14:00073877

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Bat Chiroptera dermatomycosis fungal infection Pseudogymnoascus Geomyces destructans ultraviolet UV fluorescence white-nose syndrome

Štítky

AKR, rivok

Změněno: 19. 2. 2018 10:43, doc. Mgr. Tomáš Bartonička, Ph.D.

Anotace

V originále

Definitive diagnosis of the bat disease white-nose syndrome (WNS) requires histologic analysis to identify the cutaneous erosions caused by the fungal pathogen Pseudogymnoascus [formerly Geomyces] destructans (Pd). Gross visual inspection does not distinguish bats with or without WNS, and no nonlethal, on-site, preliminary screening methods are available for WNS in bats. We demonstrate that long-wave ultraviolet (UV) light (wavelength 366–385 nm) elicits a distinct orange–yellow fluorescence in bat-wing membranes (skin) that corresponds directly with the fungal cupping erosions in histologic sections of skin that are the current gold standard for diagnosis of WNS. Between March 2009 and April 2012, wing membranes from 168 North American bat carcasses submitted to the US Geological Survey National Wildlife Health Center were examined with the use of both UV light and histology. Comparison of these techniques showed that 98.8% of the bats with foci of orange–yellow wing fluorescence (n580) were WNS-positive based on histologic diagnosis; bat wings that did not fluoresce under UV light (n588) were all histologically negative for WNS lesions. Punch biopsy samples as small as 3 mm taken from areas of wing with UV fluorescence were effective for identifying lesions diagnostic for WNS by histopathology. In a nonlethal biopsy-based study of 62 bats sampled (4-mm diameter) in hibernacula of the Czech Republic during 2012, 95.5% of fluorescent (n522) and 100% of nonfluorescent (n540) wing samples were confirmed by histopathology to be WNS positive and negative, respectively. This evidence supports use of longwave UV light as a nonlethal and field-applicable method to screen bats for lesions indicative of WNS. Further, UV fluorescence can be used to guide targeted, nonlethal biopsy sampling for follow-up molecular testing, fungal culture analysis, and histologic confirmation of WNS.

Návaznosti

GAP506/12/1064, projekt VaV

Název: Adaptace netopýrů na plísňové onemocnění geomykózu

Investor: Grantová agentura ČR, Adaptace netopýrů na plísňové onemocnění geomykózou

Citovat

TURNER, Gregory; Carol METEYER; Hazel BARTON; John GUMBS; DeeAnn REEDER; Barrie OVERTON; Hana BANĎOUCHOVÁ; Tomáš BARTONIČKA; Natália MARTÍNKOVÁ; Jiří PIKULA; Jan ZUKAL a David BLEHERT. NONLETHAL SCREENING OF BAT-WING SKIN WITH THE USE OF ULTRAVIOLET FLUORESCENCE TO DETECT LESIONS INDICATIVE OF WHITE-NOSE SYNDROME. Journal of Wildlife Diseases. Wildlife Disease Association, 2014, roč. 50, č. 3, s. 566-573. ISSN 0090-3558. Dostupné z: https://doi.org/10.7589/2014-03-058.

@article{1198931,
   author = {Turner, Gregory and Meteyer, Carol and Barton, Hazel and Gumbs, John and Reeder, DeeAnn and Overton, Barrie and Banďouchová, Hana and Bartonička, Tomáš and Martínková, Natália and Pikula, Jiří and Zukal, Jan and Blehert, David},
   article_number = {3},
   doi = {https://doi.org/10.7589/2014-03-058},
   keywords = {Bat Chiroptera dermatomycosis fungal infection Pseudogymnoascus Geomyces destructans ultraviolet UV fluorescence white-nose syndrome},
   language = {eng},
   issn = {0090-3558},
   journal = {Journal of Wildlife Diseases},
   title = {NONLETHAL SCREENING OF BAT-WING SKIN WITH THE USE OF ULTRAVIOLET FLUORESCENCE TO DETECT LESIONS INDICATIVE OF WHITE-NOSE SYNDROME},
   volume = {50},
   year = {2014}
}

TY  - JOUR
ID  - 1198931
AU  - Turner, Gregory - Meteyer, Carol - Barton, Hazel - Gumbs, John - Reeder, DeeAnn - Overton, Barrie - Banďouchová, Hana - Bartonička, Tomáš - Martínková, Natália - Pikula, Jiří - Zukal, Jan - Blehert, David
PY  - 2014
TI  - NONLETHAL SCREENING OF BAT-WING SKIN WITH THE USE OF ULTRAVIOLET FLUORESCENCE TO DETECT LESIONS INDICATIVE OF WHITE-NOSE SYNDROME
JF  - Journal of Wildlife Diseases
VL  - 50
IS  - 3
SP  - 566-573
EP  - 566-573
PB  - Wildlife Disease Association
SN  - 00903558
KW  - Bat Chiroptera dermatomycosis fungal infection Pseudogymnoascus Geomyces destructans ultraviolet UV fluorescence white-nose syndrome
N2  - Definitive diagnosis of the bat disease white-nose syndrome (WNS) requires histologic analysis to identify the cutaneous erosions caused by the fungal pathogen Pseudogymnoascus [formerly Geomyces] destructans (Pd). Gross visual inspection does not distinguish bats with or without WNS, and no nonlethal, on-site, preliminary screening methods are available for WNS in bats. We demonstrate that long-wave ultraviolet (UV) light (wavelength 366–385 nm) elicits a distinct orange–yellow fluorescence in bat-wing membranes (skin) that corresponds directly with the fungal cupping erosions in histologic sections of skin that are the current gold standard for diagnosis of WNS. Between March 2009 and April 2012, wing membranes from 168 North American bat carcasses submitted to the US Geological Survey National Wildlife Health Center were examined with the use of both UV light and histology. Comparison of these techniques showed that 98.8% of the bats with foci of orange–yellow wing fluorescence (n580) were WNS-positive based on histologic diagnosis; bat wings that did not fluoresce under UV light (n588) were all histologically negative for WNS lesions. Punch biopsy samples as small as 3 mm taken from areas of wing with UV fluorescence were effective for identifying lesions diagnostic for WNS by histopathology. In a nonlethal biopsy-based study of 62 bats sampled (4-mm diameter) in hibernacula of the Czech Republic during 2012, 95.5% of fluorescent (n522) and 100% of nonfluorescent (n540) wing samples were confirmed by histopathology to be WNS positive and negative, respectively. This evidence supports use of longwave UV light as a nonlethal and field-applicable method to screen bats for lesions indicative of WNS. Further, UV fluorescence can be used to guide targeted, nonlethal biopsy sampling for follow-up molecular testing, fungal culture analysis, and histologic confirmation of WNS.
ER  -

TURNER, Gregory; Carol METEYER; Hazel BARTON; John GUMBS; DeeAnn REEDER; Barrie OVERTON; Hana BANĎOUCHOVÁ; Tomáš BARTONIČKA; Natália MARTÍNKOVÁ; Jiří PIKULA; Jan ZUKAL a David BLEHERT. NONLETHAL SCREENING OF BAT-WING SKIN WITH THE USE OF ULTRAVIOLET FLUORESCENCE TO DETECT LESIONS INDICATIVE OF WHITE-NOSE SYNDROME. \textit{Journal of Wildlife Diseases}. Wildlife Disease Association, 2014, roč.~50, č.~3, s.~566-573. ISSN~0090-3558. Dostupné z: https://doi.org/10.7589/2014-03-058.

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