Comparison of the effects of silver phosphate and selenium
nanoparticles on Staphylococcus aureus growth reveals potential
for selenium particles to prevent infection

J 2014

Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection

CHUDOBOVÁ, Dagmar, Kristyna CIHALOVA, Simona DOSTALOVA, Branislav RUTTKAY-NEDECKY, Miguel Angel Merlos RODRIGO et. al.

Základní údaje

Originální název

Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection

Autoři

CHUDOBOVÁ, Dagmar (203 Česká republika), Kristyna CIHALOVA (203 Česká republika), Simona DOSTALOVA (203 Česká republika), Branislav RUTTKAY-NEDECKY (203 Česká republika), Miguel Angel Merlos RODRIGO (203 Česká republika), Kateřina TMEJOVÁ (203 Česká republika), Pavel KOPEL (203 Česká republika), Lukas NEJDL (203 Česká republika), Jiri KUDR (203 Česká republika), Jaromír GUMULEC (203 Česká republika, domácí), Soňa KŘÍŽKOVÁ (203 Česká republika), Jindřich KYNICKÝ (203 Česká republika), René KIZEK (203 Česká republika) a Vojtěch ADAM (203 Česká republika, garant)

Vydání

FEMS Microbiology Letters, Amsterdam, Elsevier Science B. V. 2014, 0378-1097

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.121

Kód RIV

RIV/00216224:14110/14:00076562

Organizační jednotka

Lékařská fakulta

UT WoS

000331783000012

Klíčová slova anglicky

BIOFILM FORMATION; IN-VITRO; ANTIBACTERIAL; MECHANISMS; GENERATION; PATHOGENS; COATINGS; EFFICACY; PROTEIN; CELLS

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 9. 2014 14:46, Soňa Böhmová

Anotace

V originále

Interactions of silver phosphate nanoparticles (SPNPs) and selenium nanoparticles (SeNPs) with Staphylococcus aureus cultures have been studied at the cellular, molecular and protein level. Significant antibacterial effects of both SPNPs and SeNPs on S. aureus were observed. At a concentration of 300 uM, SPNPs caused 37.5% inhibition of bacterial growth and SeNPs totally inhibited bacterial growth. As these effects might have been performed due to the interactions of nanoparticles with DNA and proteins, the interaction of SPNPs or SeNPs with the amplified zntR gene was studied. The presence of nanoparticles decreased the melting temperatures of the nanoparticle complexes with the zntR gene by 23% for SeNPs and by 12% for SPNPs in comparison with the control value. The concentration of bacterial metallothionein was 87% lower in bacteria after application of SPNPs (6.3 ug mg-1 protein) but was increased by 29% after addition of SeNPs (63 ug mg-1 protein) compared with the S. aureus control (49 ug mg-1 protein). Significant antimicrobial effects of the nanoparticles on bacterial growth and DNA integrity provide a promising approach to reducing the risk of bacterial infections that cannot be controlled by the usual antibiotic treatments.

Citovat

CHUDOBOVÁ, Dagmar, Kristyna CIHALOVA, Simona DOSTALOVA, Branislav RUTTKAY-NEDECKY, Miguel Angel Merlos RODRIGO, Kateřina TMEJOVÁ, Pavel KOPEL, Lukas NEJDL, Jiri KUDR, Jaromír GUMULEC, Soňa KŘÍŽKOVÁ, Jindřich KYNICKÝ, René KIZEK a Vojtěch ADAM. Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection. FEMS Microbiology Letters. Amsterdam: Elsevier Science B. V., 2014, roč. 351, č. 2, s. 195-201. ISSN 0378-1097.

@article{1199010,
   author = {Chudobová, Dagmar and Cihalova, Kristyna and Dostalova, Simona and RuttkayandNedecky, Branislav and Rodrigo, Miguel Angel Merlos and Tmejová, Kateřina and Kopel, Pavel and Nejdl, Lukas and Kudr, Jiri and Gumulec, Jaromír and Křížková, Soňa and Kynický, Jindřich and Kizek, René and Adam, Vojtěch},
   article_location = {Amsterdam},
   article_number = {2},
   keywords = {BIOFILM FORMATION; IN-VITRO; ANTIBACTERIAL; MECHANISMS; GENERATION; PATHOGENS; COATINGS; EFFICACY; PROTEIN; CELLS},
   language = {eng},
   issn = {0378-1097},
   journal = {FEMS Microbiology Letters},
   title = {Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection},
   volume = {351},
   year = {2014}
}

TY  - JOUR
ID  - 1199010
AU  - Chudobová, Dagmar - Cihalova, Kristyna - Dostalova, Simona - Ruttkay-Nedecky, Branislav - Rodrigo, Miguel Angel Merlos - Tmejová, Kateřina - Kopel, Pavel - Nejdl, Lukas - Kudr, Jiri - Gumulec, Jaromír - Křížková, Soňa - Kynický, Jindřich - Kizek, René - Adam, Vojtěch
PY  - 2014
TI  - Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection
JF  - FEMS Microbiology Letters
VL  - 351
IS  - 2
SP  - 195-201
EP  - 195-201
PB  - Elsevier Science B. V.
SN  - 03781097
KW  - BIOFILM FORMATION
KW  - IN-VITRO
KW  - ANTIBACTERIAL
KW  - MECHANISMS
KW  - GENERATION
KW  - PATHOGENS
KW  - COATINGS
KW  - EFFICACY
KW  - PROTEIN
KW  - CELLS
N2  - Interactions of silver phosphate nanoparticles (SPNPs) and selenium nanoparticles (SeNPs) with Staphylococcus aureus cultures have been studied at the cellular, molecular and protein level. Significant antibacterial effects of both SPNPs and SeNPs on S. aureus were observed. At a concentration of 300 uM, SPNPs caused 37.5% inhibition of bacterial growth and SeNPs totally inhibited bacterial growth. As these effects might have been performed due to the interactions of nanoparticles with DNA and proteins, the interaction of SPNPs or SeNPs with the amplified zntR gene was studied. The presence of nanoparticles decreased the melting temperatures of the nanoparticle complexes with the zntR gene by 23% for SeNPs and by 12% for SPNPs in comparison with the control value. The concentration of bacterial metallothionein was 87% lower in bacteria after application of SPNPs (6.3 ug mg-1 protein) but was increased by 29% after addition of SeNPs (63 ug mg-1 protein) compared with the S. aureus control (49 ug mg-1 protein). Significant antimicrobial effects of the nanoparticles on bacterial growth and DNA integrity provide a promising approach to reducing the risk of bacterial infections that cannot be controlled by the usual antibiotic treatments.
ER  -

CHUDOBOVÁ, Dagmar, Kristyna CIHALOVA, Simona DOSTALOVA, Branislav RUTTKAY-NEDECKY, Miguel Angel Merlos RODRIGO, Kateřina TMEJOVÁ, Pavel KOPEL, Lukas NEJDL, Jiri KUDR, Jaromír GUMULEC, Soňa KŘÍŽKOVÁ, Jindřich KYNICKÝ, René KIZEK a Vojtěch ADAM. Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection. \textit{FEMS Microbiology Letters}. Amsterdam: Elsevier Science B. V., 2014, roč.~351, č.~2, s.~195-201. ISSN~0378-1097.

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