Effect of Ca2+ Efflux Pathway Distribution and Exogenous Ca2+
Buffers on Intracellular Ca2+ Dynamics in the Rat Ventricular
Myocyte: A Simulation Study

J 2014

Effect of Ca2+ Efflux Pathway Distribution and Exogenous Ca2+ Buffers on Intracellular Ca2+ Dynamics in the Rat Ventricular Myocyte: A Simulation Study

PÁSEK, Michal; Jiří ŠIMURDA a Clive H. ORCHARD

Základní údaje

Originální název

Effect of Ca2+ Efflux Pathway Distribution and Exogenous Ca2+ Buffers on Intracellular Ca2+ Dynamics in the Rat Ventricular Myocyte: A Simulation Study

Autoři

PÁSEK, Michal; Jiří ŠIMURDA a Clive H. ORCHARD

Vydání

BioMed Research International, New York, Hindawi Publishing Corporation, 2014, 2314-6133

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.579

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/14:00080146

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

SARCOPLASMIC-RETICULUM; T-TUBULES; CARDIAC MYOCYTES; SURFACE-MEMBRANE; CALCIUM-RELEASE; INACTIVATION; EXCHANGE; CHANNELS; MUSCLE

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 10. 2014 15:49, Soňa Böhmová

Anotace

V originále

We have used a previously published computer model of the rat cardiac ventricular myocyte to investigate the effect of changing the distribution of Ca2+ efflux pathways (SERCA, Na+/Ca2+ exchange, and sarcolemmal Ca2+ ATPase) between the dyad and bulk cytoplasm and the effect of adding exogenous Ca2+ buffers (BAPTA or EGTA), which are used experimentally to differentially buffer Ca2+ in the dyad and bulk cytoplasm, on cellular Ca2+ cycling. Increasing the dyadic fraction of a particular Ca2+ efflux pathway increases the amount of Ca2+ removed by that pathway, with corresponding changes in Ca2+ efflux from the bulk cytoplasm. The magnitude of these effects varies with the proportion of the total Ca2+ removed from the cytoplasm by that pathway. Differences in the response to EGTA and BAPTA, including changes in Ca2+-dependent inactivation of the L-type Ca2+ current, resulted from the buffers acting as slow and fast "shuttles," respectively, removing Ca2+ from the dyadic space. The data suggest that complex changes in dyadic Ca2+ and cellular Ca2+ cycling occur as a result of changes in the location of Ca2+ removal pathways or the presence of exogenous Ca2+ buffers, although changing the distribution of Ca2+ efflux pathways has relatively small effects on the systolic Ca2+ transient.

Návaznosti

NT14301, projekt VaV

Název: Vliv ethanolu a jeho metabolitu acetaldehydu na srdeční inward rectifier draslíkové proudy: vztah k fibrilaci síní po konzumaci alkoholu?

Investor: Ministerstvo zdravotnictví ČR, Vliv ethanolu a jeho metabolitu acetaldehydu na srdeční inward rectifier draslíkové proudy: vztah k fibrilaci síní po konzumaci alkoholu?

Citovat

PÁSEK, Michal; Jiří ŠIMURDA a Clive H. ORCHARD. Effect of Ca2+ Efflux Pathway Distribution and Exogenous Ca2+ Buffers on Intracellular Ca2+ Dynamics in the Rat Ventricular Myocyte: A Simulation Study. BioMed Research International. New York: Hindawi Publishing Corporation, 2014, roč. 2014, č. 920208, s. 1-12. ISSN 2314-6133. Dostupné z: https://doi.org/10.1155/2014/920208.

@article{1202680,
   author = {Pásek, Michal and Šimurda, Jiří and Orchard, Clive H.},
   article_location = {New York},
   article_number = {920208},
   doi = {https://doi.org/10.1155/2014/920208},
   keywords = {SARCOPLASMIC-RETICULUM; T-TUBULES; CARDIAC MYOCYTES; SURFACE-MEMBRANE; CALCIUM-RELEASE; INACTIVATION; EXCHANGE; CHANNELS; MUSCLE},
   language = {eng},
   issn = {2314-6133},
   journal = {BioMed Research International},
   title = {Effect of Ca2+ Efflux Pathway Distribution and Exogenous Ca2+ Buffers on Intracellular Ca2+ Dynamics in the Rat Ventricular Myocyte: A Simulation Study},
   volume = {2014},
   year = {2014}
}

TY  - JOUR
ID  - 1202680
AU  - Pásek, Michal - Šimurda, Jiří - Orchard, Clive H.
PY  - 2014
TI  - Effect of Ca2+ Efflux Pathway Distribution and Exogenous Ca2+ Buffers on Intracellular Ca2+ Dynamics in the Rat Ventricular Myocyte: A Simulation Study
JF  - BioMed Research International
VL  - 2014
IS  - 920208
SP  - 1-12
EP  - 1-12
PB  - Hindawi Publishing Corporation
SN  - 23146133
KW  - SARCOPLASMIC-RETICULUM
KW  - T-TUBULES
KW  - CARDIAC MYOCYTES
KW  - SURFACE-MEMBRANE
KW  - CALCIUM-RELEASE
KW  - INACTIVATION
KW  - EXCHANGE
KW  - CHANNELS
KW  - MUSCLE
N2  - We have used a previously published computer model of the rat cardiac ventricular myocyte to investigate the effect of changing the distribution of Ca2+ efflux pathways (SERCA, Na+/Ca2+ exchange, and sarcolemmal Ca2+ ATPase) between the dyad and bulk cytoplasm and the effect of adding exogenous Ca2+ buffers (BAPTA or EGTA), which are used experimentally to differentially buffer Ca2+ in the dyad and bulk cytoplasm, on cellular Ca2+ cycling. Increasing the dyadic fraction of a particular Ca2+ efflux pathway increases the amount of Ca2+ removed by that pathway, with corresponding changes in Ca2+ efflux from the bulk cytoplasm. The magnitude of these effects varies with the proportion of the total Ca2+ removed from the cytoplasm by that pathway. Differences in the response to EGTA and BAPTA, including changes in Ca2+-dependent inactivation of the L-type Ca2+ current, resulted from the buffers acting as slow and fast "shuttles," respectively, removing Ca2+ from the dyadic space. The data suggest that complex changes in dyadic Ca2+ and cellular Ca2+ cycling occur as a result of changes in the location of Ca2+ removal pathways or the presence of exogenous Ca2+ buffers, although changing the distribution of Ca2+ efflux pathways has relatively small effects on the systolic Ca2+ transient.
ER  -

PÁSEK, Michal; Jiří ŠIMURDA a Clive H. ORCHARD. Effect of Ca2+ Efflux Pathway Distribution and Exogenous Ca2+ Buffers on Intracellular Ca2+ Dynamics in the Rat Ventricular Myocyte: A Simulation Study. \textit{BioMed Research International}. New York: Hindawi Publishing Corporation, 2014, roč.~2014, č.~920208, s.~1-12. ISSN~2314-6133. Dostupné z: https://doi.org/10.1155/2014/920208.

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