Cell proliferation and apoptosis in the primary enamel knot
measured by flow cytometry of laser microdissected samples

J 2010

Cell proliferation and apoptosis in the primary enamel knot measured by flow cytometry of laser microdissected samples

MATALOVÁ, Eva, Lenka ZDRAŽILOVÁ DUBSKÁ, Jana FLEISCHMANNOVÁ, I CHLASTAKOVA, Eva JANEČKOVÁ et. al.

Základní údaje

Originální název

Cell proliferation and apoptosis in the primary enamel knot measured by flow cytometry of laser microdissected samples

Autoři

MATALOVÁ, Eva, Lenka ZDRAŽILOVÁ DUBSKÁ, Jana FLEISCHMANNOVÁ, I CHLASTAKOVA, Eva JANEČKOVÁ a A S TUCKER

Vydání

Archives of Oral Biology, Amsterodam, Elsevier, 2010, 0003-9969

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.463

DOI

http://dx.doi.org/10.1016/j.archoralbio.2010.05.007

UT WoS

000280465300005

Změněno: 15. 10. 2014 14:22, MUDr. RNDr. Michal Řiháček, Ph.D., EuSpLM

Anotace

V originále

Laser capture microdissection (LCM) uniquely allows the selection of specific cell populations from histological sections These selected cells are then catapulted into a test tube without any contamination from surrounding tissues During the last ten years, many significant results have been achieved, particularly at the level of DNA and RNA where amplification techniques are available However, where amplification procedures are difficult, the benefits of LCM diminish To overcome such difficulties, a novel approach, combining laser capture microdissection and flow cytometry, has been tested here for detection of apoptosis and proliferation in tissue bound cell populations without any amplification steps The mouse cap stage molar tooth germ was used as a model At the centre of the inner enamel epithelium, the primary enamel knot is a clearly defined apoptotic population with minimal proliferation, flanked by the highly proliferative cervical loops on each side Thus within the tooth germ epithelium at this stage, two distinct populations of cells are found side by side These populations were selected by laser capture microdissection and then analysed by flow cytometry for apoptosis and proliferation Flow cytometric results correlated well with immunohistochemical findings, demonstrating the success and sensitivity of this combined procedure (C) 2010 Elsevier Ltd All rights reserved

Citovat

MATALOVÁ, Eva, Lenka ZDRAŽILOVÁ DUBSKÁ, Jana FLEISCHMANNOVÁ, I CHLASTAKOVA, Eva JANEČKOVÁ a A S TUCKER. Cell proliferation and apoptosis in the primary enamel knot measured by flow cytometry of laser microdissected samples. Archives of Oral Biology. Amsterodam: Elsevier, 2010, roč. 55, č. 8, s. 570-575. ISSN 0003-9969. Dostupné z: https://dx.doi.org/10.1016/j.archoralbio.2010.05.007.

@article{1202739,
   author = {Matalová, Eva and Zdražilová Dubská, Lenka and Fleischmannová, Jana and Chlastakova, I and Janečková, Eva and Tucker, A S},
   article_location = {Amsterodam},
   article_number = {8},
   doi = {http://dx.doi.org/10.1016/j.archoralbio.2010.05.007},
   issn = {0003-9969},
   journal = {Archives of Oral Biology},
   title = {Cell proliferation and apoptosis in the primary enamel knot measured by flow cytometry of laser microdissected samples},
   volume = {55},
   year = {2010}
}

TY  - JOUR
ID  - 1202739
AU  - Matalová, Eva - Zdražilová Dubská, Lenka - Fleischmannová, Jana - Chlastakova, I - Janečková, Eva - Tucker, A S
PY  - 2010
TI  - Cell proliferation and apoptosis in the primary enamel knot measured by flow cytometry of laser microdissected samples
JF  - Archives of Oral Biology
VL  - 55
IS  - 8
SP  - 570-575
EP  - 570-575
PB  - Elsevier
SN  - 00039969
N2  - Laser capture microdissection (LCM) uniquely allows the selection of specific cell populations from histological sections These selected cells are then catapulted into a test tube without any contamination from surrounding tissues During the last ten years, many significant results have been achieved, particularly at the level of DNA and RNA where amplification techniques are available However, where amplification procedures are difficult, the benefits of LCM diminish To overcome such difficulties, a novel approach, combining laser capture microdissection and flow cytometry, has been tested here for detection of apoptosis and proliferation in tissue bound cell populations without any amplification steps The mouse cap stage molar tooth germ was used as a model At the centre of the inner enamel epithelium, the primary enamel knot is a clearly defined apoptotic population with minimal proliferation, flanked by the highly proliferative cervical loops on each side Thus within the tooth germ epithelium at this stage, two distinct populations of cells are found side by side These populations were selected by laser capture microdissection and then analysed by flow cytometry for apoptosis and proliferation Flow cytometric results correlated well with immunohistochemical findings, demonstrating the success and sensitivity of this combined procedure (C) 2010 Elsevier Ltd All rights reserved
ER  -

MATALOVÁ, Eva, Lenka ZDRAŽILOVÁ DUBSKÁ, Jana FLEISCHMANNOVÁ, I CHLASTAKOVA, Eva JANEČKOVÁ a A S TUCKER. Cell proliferation and apoptosis in the primary enamel knot measured by flow cytometry of laser microdissected samples. \textit{Archives of Oral Biology}. Amsterodam: Elsevier, 2010, roč.~55, č.~8, s.~570-575. ISSN~0003-9969. Dostupné z: https://dx.doi.org/10.1016/j.archoralbio.2010.05.007.

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