DROPLET DIGITAL PCR (DDPCR) AND REAL-TIME PCR (RQ-PCR): A HEAD
TO HEAD COMPARISON FOR MRD DETECTION IN MYELOMA (MM) MANTLE
CELL LYMPHOMA (MCL) AND FOLLICULAR LYMPHOMA (FL)

a 2014

DROPLET DIGITAL PCR (DDPCR) AND REAL-TIME PCR (RQ-PCR): A HEAD TO HEAD COMPARISON FOR MRD DETECTION IN MYELOMA (MM) MANTLE CELL LYMPHOMA (MCL) AND FOLLICULAR LYMPHOMA (FL)

DRANDI, Daniela; Lenka BEŠŠE; Simone FERRERO; Nadia DANI; Roberto PASSERA et al.

Základní údaje

Originální název

DROPLET DIGITAL PCR (DDPCR) AND REAL-TIME PCR (RQ-PCR): A HEAD TO HEAD COMPARISON FOR MRD DETECTION IN MYELOMA (MM) MANTLE CELL LYMPHOMA (MCL) AND FOLLICULAR LYMPHOMA (FL)

Autoři

Vydání

19th Congress of the European Hematology Association; June 12-15, 2014; Milano, Italy, 2014

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Itálie

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/14:00076847

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Lymphoproliferative disorder; Minimal residual disease (MRD); PCR; Quantitative molecular analysis

Štítky

EL OK

Změněno: 15. 10. 2014 14:24, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Minimal residual disease (MRD) detection is a well described prognostic tool for response evaluation in several hematological malignancies. Real time quantitative PCR (RQ-PCR) has an established role in Mantle cell Lymphoma (MCL), Follicular Lymphoma (FL) and Multiple Myeloma (MM). However, RQ-PCR-based MRD detection is a relative quantification approach, since it requires a reference standard curve. Droplet Digital PCR (ddPCR), based on wide sample partition and Poisson’s statistic, allows for a trustworthy absolute quantification.

Citovat

DRANDI, Daniela; Lenka BEŠŠE; Simone FERRERO; Nadia DANI; Roberto PASSERA; Barbara MANTOAN; Manuela GAMBELLA; Elona SARACI; Luigia MONITILLO; Elisa GENUARDI; Daniela BARBERO; Paola GHIONE; Guglielmo GUARONA; Paola OMEDÈ; Davide BARBERIO; Roman HÁJEK; Umberto VITOLO; Sergio CORTELAZZO; Antonio PALUMBO; Mario BOCCADORO; Giorgio INGHIRAMI a Marco LADETTO. DROPLET DIGITAL PCR (DDPCR) AND REAL-TIME PCR (RQ-PCR): A HEAD TO HEAD COMPARISON FOR MRD DETECTION IN MYELOMA (MM) MANTLE CELL LYMPHOMA (MCL) AND FOLLICULAR LYMPHOMA (FL). In 19th Congress of the European Hematology Association; June 12-15, 2014; Milano, Italy. 2014.

@proceedings{1202740,
   author = {Drandi, Daniela and Bešše, Lenka and Ferrero, Simone and Dani, Nadia and Passera, Roberto and Mantoan, Barbara and Gambella, Manuela and Saraci, Elona and Monitillo, Luigia and Genuardi, Elisa and Barbero, Daniela and Ghione, Paola and Guarona, Guglielmo and Omedè, Paola and Barberio, Davide and Hájek, Roman and Vitolo, Umberto and Cortelazzo, Sergio and Palumbo, Antonio and Boccadoro, Mario and Inghirami, Giorgio and Ladetto, Marco},
   booktitle = {19th Congress of the European Hematology Association; June 12-15, 2014; Milano, Italy},
   keywords = {Lymphoproliferative disorder; Minimal residual disease (MRD); PCR; Quantitative molecular analysis},
   language = {eng},
   title = {DROPLET DIGITAL PCR (DDPCR) AND REAL-TIME PCR (RQ-PCR): A HEAD TO HEAD COMPARISON FOR MRD DETECTION IN MYELOMA (MM) MANTLE CELL LYMPHOMA (MCL) AND FOLLICULAR LYMPHOMA (FL)},
   year = {2014}
}

TY  - CONF
ID  - 1202740
AU  - Drandi, Daniela - Bešše, Lenka - Ferrero, Simone - Dani, Nadia - Passera, Roberto - Mantoan, Barbara - Gambella, Manuela - Saraci, Elona - Monitillo, Luigia - Genuardi, Elisa - Barbero, Daniela - Ghione, Paola - Guarona, Guglielmo - Omedè, Paola - Barberio, Davide - Hájek, Roman - Vitolo, Umberto - Cortelazzo, Sergio - Palumbo, Antonio - Boccadoro, Mario - Inghirami, Giorgio - Ladetto, Marco
PY  - 2014
TI  - DROPLET DIGITAL PCR (DDPCR) AND REAL-TIME PCR (RQ-PCR): A HEAD TO HEAD COMPARISON FOR MRD DETECTION IN MYELOMA (MM) MANTLE CELL LYMPHOMA (MCL) AND FOLLICULAR LYMPHOMA (FL)
KW  - Lymphoproliferative disorder
KW  - Minimal residual disease (MRD)
KW  - PCR
KW  - Quantitative molecular analysis
N2  - Minimal residual disease (MRD) detection is a well described prognostic tool for response evaluation in several hematological malignancies. Real time quantitative PCR (RQ-PCR) has an established role in Mantle cell Lymphoma (MCL), Follicular Lymphoma (FL) and Multiple Myeloma (MM). However, RQ-PCR-based MRD detection is a relative quantification approach, since it requires a reference standard curve. Droplet Digital PCR (ddPCR), based on wide sample partition and Poisson’s statistic, allows for a trustworthy absolute quantification.
ER  -

DRANDI, Daniela; Lenka BEŠŠE; Simone FERRERO; Nadia DANI; Roberto PASSERA; Barbara MANTOAN; Manuela GAMBELLA; Elona SARACI; Luigia MONITILLO; Elisa GENUARDI; Daniela BARBERO; Paola GHIONE; Guglielmo GUARONA; Paola OMEDÈ; Davide BARBERIO; Roman HÁJEK; Umberto VITOLO; Sergio CORTELAZZO; Antonio PALUMBO; Mario BOCCADORO; Giorgio INGHIRAMI a Marco LADETTO. DROPLET DIGITAL PCR (DDPCR) AND REAL-TIME PCR (RQ-PCR): A HEAD TO HEAD COMPARISON FOR MRD DETECTION IN MYELOMA (MM) MANTLE CELL LYMPHOMA (MCL) AND FOLLICULAR LYMPHOMA (FL). In \textit{19th Congress of the European Hematology Association; June 12-15, 2014; Milano, Italy}. 2014.

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