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@proceedings{1202744,
author = {Drandi, Daniela and Bešše, Lenka and Ferrero, Simone and Passera, Roberto and Mantoan, Barbara and Monitillo, Luigia and Gambella, Manuela and Omedè, Paola and Hájek, Roman and Vitolo, Umberto and Palumbo, Antonio and Cortelazzo, Sergio and Boccadoro, Mario and Inghirami, Giorgio and Ladetto, Marco},
booktitle = {Digital PCR Conference: Technologies and Tools for Precision Diagnostics; October 6-8,2014, La Jolla, San Diego, California},
keywords = {ddPCR; multiple myeloma; Non-Hodgkin lymphoma; RQ-PCR; minimal residual disease},
language = {eng},
title = {Droplet digital PCR improves minimal residual disease monitoring in mature lymphoid tumors},
year = {2014}
}
TY - CONF
ID - 1202744
AU - Drandi, Daniela - Bešše, Lenka - Ferrero, Simone - Passera, Roberto - Mantoan, Barbara - Monitillo, Luigia - Gambella, Manuela - Omedè, Paola - Hájek, Roman - Vitolo, Umberto - Palumbo, Antonio - Cortelazzo, Sergio - Boccadoro, Mario - Inghirami, Giorgio - Ladetto, Marco
PY - 2014
TI - Droplet digital PCR improves minimal residual disease monitoring in mature lymphoid tumors
KW - ddPCR
KW - multiple myeloma
KW - Non-Hodgkin lymphoma
KW - RQ-PCR
KW - minimal residual disease
N2 - Minimal residual disease (MRD) detection allowed acquisition of valuable prognostic information in several mature lymphoid disorders with a considerable impact on clinical research (Ferrero S. 2011, Pott C. 2010). Although different methods can be used for MRD quantification, including flow cytometry (FC), real-time quantitative polymerase chain reaction (qPCR) remains the most validated and standardized method (Ladetto M. 2013, Pott C. 2011). However, despite remarkable sensitivity and specificity, qPCR has some drawbacks mainly related to the need for a reference standard curve, performed by target serial dilutions. The introduction of droplet digital PCR (ddPCR) has allowed to overcome this limitation, since its absolute quantification competence allows to avoid the need for a reference standard curve. Based on these considerations, we sought to verify the utility of ddPCR as a MRD monitoring tool and to verify whether ddPCR could overcome some qPCR limitations without mislay its critical advantages. We compared the two approaches, in the context of MRD evaluation, in multiple myeloma (MM), mantle cell lymphoma (MCL), and follicular lymphoma (FL) patients enrolled in prospective clinical trials and selected for having the immunoglobuline gene (IGH) (MM and MCL) or Bcl-2/MBR (FL) molecular marker at diagnosis.
ER -
DRANDI, Daniela, Lenka BEŠŠE, Simone FERRERO, Roberto PASSERA, Barbara MANTOAN, Luigia MONITILLO, Manuela GAMBELLA, Paola OMEDÈ, Roman HÁJEK, Umberto VITOLO, Antonio PALUMBO, Sergio CORTELAZZO, Mario BOCCADORO, Giorgio INGHIRAMI a Marco LADETTO. Droplet digital PCR improves minimal residual disease monitoring in mature lymphoid tumors. In \textit{Digital PCR Conference: Technologies and Tools for Precision Diagnostics; October 6-8,2014, La Jolla, San Diego, California}. 2014.
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