DNA methylation levels of selected genes in human pluripotent
stem cell lines intended for hematopoietic differentiation.

a 2014

DNA methylation levels of selected genes in human pluripotent stem cell lines intended for hematopoietic differentiation.

TESAŘOVÁ, Lenka; Pavel ŠIMARA; Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ

Základní údaje

Originální název

DNA methylation levels of selected genes in human pluripotent stem cell lines intended for hematopoietic differentiation.

Autoři

TESAŘOVÁ, Lenka; Pavel ŠIMARA; Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ

Vydání

2014 EMBO Conference Stem Cells in Cancer and Regenerative medicine, Heidelberg, Germany, Oct 9-12, 2014. 2014

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10601 Cell biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Fakulta informatiky

Klíčová slova česky

pluripotentní kmenové buňky; metylace promotorové DNA; hematopoetický vývoj

Klíčová slova anglicky

pluripotent stem cells; promotor DNA methylation; hematopoietic development

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 3. 2018 10:01, Mgr. Pavel Šimara, Ph.D.

Anotace

V originále

Generation of hematopoietic stem cells from human pluripotent stem cells (hPSCs) promise many applications in regenerative medicine. Nevertheless, the limitation is variable differentiation efficiency of hPSCs reasoned by abberant molecular signature and epigenetic status of developing hPSCs. We evaluated DNA methylation status of selected genes in several hPSC lines to determine if this DNA modification is associated with the variabilty of hPSC lines and with their hematopoietic differentation potential. Using MethylScreen technology, promotor DNA methylation was analysed in human embryonic stem cell (hESC) lines (CCTL-12, CCTL-14) and human induced pluripotent stem cell (hiPSC) lines derived from human fibroblasts (STENF, IPSCF, AM13). The gene panel included markers for pluripotent cells (UTF1, SOX2), embryonic germ layers (Brachyury T, FOXA2, PAX6) and blood progenitors (SOX17, RUNX1, GATA2, CD34) and genes with described DNA methylation variability in hPSCs (TCERG1L, TSPYL5). In hESC lines all analysed genes were found to be unmethylated with the exception of fully methylated TSPYL5. For hiPSC lines, STENF and IPSCF showed decreased TSPYL5 methylation level and IPSCF was further characterised by intermediate methylation of UTF1. The highest level of DNA methylation was found in AM13, in addition to full methylation of TSPYL5, up to 20 percent of hypermethylation was detected for other five genes. In conclusion, it was demonstrated that there are differences in promotor DNA methylation of selected genes between hiPSC and hESC lines. Differences were detected both in residual DNA methylation of somatic genes and in DNA methylation level of UTF1 and TSPYL5, which suggests these two genes as putative markers linked to the pluripotency state. The association of DNA methylation status in hPSC lines with their ability to differentiate into blood progenitors will be further studied.

Návaznosti

CZ.1.07/2.3.00/30.0030, interní kód MU

Název: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Rozvoj lidských zdrojů pro oblast buněčné biologie, 2.3 Lidské zdroje ve výzkumu a vývoji

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

TESAŘOVÁ, Lenka; Pavel ŠIMARA; Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ. DNA methylation levels of selected genes in human pluripotent stem cell lines intended for hematopoietic differentiation. In 2014 EMBO Conference Stem Cells in Cancer and Regenerative medicine, Heidelberg, Germany, Oct 9-12, 2014. 2014.

@proceedings{1202835,
   author = {Tesařová, Lenka and Šimara, Pavel and Stejskal, Stanislav and Krontorád Koutná, Irena},
   booktitle = {2014 EMBO Conference Stem Cells in Cancer and Regenerative medicine, Heidelberg, Germany, Oct 9-12, 2014.},
   keywords = {pluripotent stem cells; promotor DNA methylation; hematopoietic development},
   language = {eng},
   title = {DNA methylation levels of selected genes in human pluripotent stem cell lines intended for hematopoietic differentiation.},
   year = {2014}
}

TY  - CONF
ID  - 1202835
AU  - Tesařová, Lenka - Šimara, Pavel - Stejskal, Stanislav - Krontorád Koutná, Irena
PY  - 2014
TI  - DNA methylation levels of selected genes in human pluripotent stem cell lines intended for hematopoietic differentiation.
KW  - pluripotent stem cells
KW  - promotor DNA methylation
KW  - hematopoietic development
N2  - Generation of hematopoietic stem cells from human pluripotent stem cells (hPSCs) promise many applications in regenerative medicine. Nevertheless, the limitation is variable differentiation efficiency of hPSCs reasoned by abberant molecular signature and epigenetic status of developing hPSCs. We evaluated DNA methylation status of selected genes in several hPSC lines to determine if this DNA modification is associated with the variabilty of hPSC lines and with their hematopoietic differentation potential. Using MethylScreen technology, promotor DNA methylation was analysed in human embryonic stem cell (hESC) lines (CCTL-12, CCTL-14) and human induced pluripotent stem cell (hiPSC) lines derived from human fibroblasts (STENF, IPSCF, AM13). The gene panel included markers for pluripotent cells (UTF1, SOX2), embryonic germ layers (Brachyury T, FOXA2, PAX6) and blood progenitors (SOX17, RUNX1, GATA2, CD34) and genes with described DNA methylation variability in hPSCs (TCERG1L, TSPYL5). In hESC lines all analysed genes were found to be unmethylated with the exception of fully methylated TSPYL5. For hiPSC lines, STENF and IPSCF showed decreased TSPYL5 methylation level and IPSCF was further characterised by intermediate methylation of UTF1. The highest level of DNA methylation was found in AM13, in addition to full methylation of TSPYL5, up to 20 percent of hypermethylation was detected for other five genes. In conclusion, it was demonstrated that there are differences in promotor DNA methylation of selected genes between hiPSC and hESC lines. Differences were detected both in residual DNA methylation of somatic genes and in DNA methylation level of UTF1 and TSPYL5, which suggests these two genes as putative markers linked to the pluripotency state. The association of DNA methylation status in hPSC lines with their ability to differentiate into blood progenitors will be further studied.
ER  -

TESAŘOVÁ, Lenka; Pavel ŠIMARA; Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ. DNA methylation levels of selected genes in human pluripotent stem cell lines intended for hematopoietic differentiation. In \textit{2014 EMBO Conference Stem Cells in Cancer and Regenerative medicine, Heidelberg, Germany, Oct 9-12, 2014.}. 2014.

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