Haematopoietic developmental potential of human pluripotent
stem cell lines

J 2014

Haematopoietic developmental potential of human pluripotent stem cell lines

TESAŘOVÁ, Lenka; Pavel ŠIMARA; Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ

Základní údaje

Originální název

Haematopoietic developmental potential of human pluripotent stem cell lines

Autoři

TESAŘOVÁ, Lenka; Pavel ŠIMARA; Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ

Vydání

Folia biologica, Praha, Univerzita Karlova, 2014, 0015-5500

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.000

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14330/14:00073970

Organizační jednotka

Fakulta informatiky

UT WoS

000343275800015

Klíčová slova česky

lidské pluripotentní kmenové buňky; hematopoetický vývoj; embryoidní tělíska; CD34+ buňky

Klíčová slova anglicky

human pluripotent stem cells; hematopoietic development; embryoid bodies; CD34+ cells

Štítky

cbia-web

Změněno: 2. 3. 2018 10:06, Mgr. Pavel Šimara, Ph.D.

Anotace

V originále

The generation of hematopoietic progenitors from human pluripotent stem cells (hPSCs) presents great promise for cell-replacement therapies. However, current protocols for hematopoietic differentiation of hPSCs suffer from low efficiency and functional defects in the derived cells. The technology is also limited by variable ability of hPSC lines to generate blood cells in vitro. To address this issue, methodologies for hematopoietic differentiation in feeder-free conditions were applied to available human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) lines in this study. It was found that these cell lines did not generate hematopoietic progenitors in such an extent as H1 and H9 hESC lines used for this purpose in the vast majority of relevant studies. These results suggest that for clinical application of blood cells derived from hPSCs, possibly from autologous hiPSCs, it is necessary to overcome the variability in the hematopoietic developmental potential of individual hPSC lines.

Návaznosti

CZ.1.07/2.3.00/30.0030, interní kód MU

Název: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Rozvoj lidských zdrojů pro oblast buněčné biologie, 2.3 Lidské zdroje ve výzkumu a vývoji

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

TESAŘOVÁ, Lenka; Pavel ŠIMARA; Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ. Haematopoietic developmental potential of human pluripotent stem cell lines. Folia biologica. Praha: Univerzita Karlova, 2014, roč. 60, č. 1, s. 90-94. ISSN 0015-5500.

@article{1202839,
   author = {Tesařová, Lenka and Šimara, Pavel and Stejskal, Stanislav and Krontorád Koutná, Irena},
   article_location = {Praha},
   article_number = {1},
   keywords = {human pluripotent stem cells; hematopoietic development; embryoid bodies; CD34+ cells},
   language = {eng},
   issn = {0015-5500},
   journal = {Folia biologica},
   title = {Haematopoietic developmental potential of human pluripotent stem cell lines},
   volume = {60},
   year = {2014}
}

TY  - JOUR
ID  - 1202839
AU  - Tesařová, Lenka - Šimara, Pavel - Stejskal, Stanislav - Krontorád Koutná, Irena
PY  - 2014
TI  - Haematopoietic developmental potential of human pluripotent stem cell lines
JF  - Folia biologica
VL  - 60
IS  - 1
SP  - 90-94
EP  - 90-94
PB  - Univerzita Karlova
SN  - 00155500
KW  - human pluripotent stem cells
KW  - hematopoietic development
KW  - embryoid bodies
KW  - CD34+ cells
N2  - The generation of hematopoietic progenitors from human pluripotent stem cells (hPSCs) presents great promise for cell-replacement therapies. However, current protocols for hematopoietic differentiation of hPSCs suffer from low efficiency and functional defects in the derived cells. The technology is also limited by variable ability of hPSC lines to generate blood cells in vitro. To address this issue, methodologies for hematopoietic differentiation in feeder-free conditions were applied to available human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) lines in this study. It was found that these cell lines did not generate hematopoietic progenitors in such an extent as H1 and H9 hESC lines used for this purpose in the vast majority of relevant studies. These results suggest that for clinical application of blood cells derived from hPSCs, possibly from autologous hiPSCs, it is necessary to overcome the variability in the hematopoietic developmental potential of individual hPSC lines.
ER  -

TESAŘOVÁ, Lenka; Pavel ŠIMARA; Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ. Haematopoietic developmental potential of human pluripotent stem cell lines. \textit{Folia biologica}. Praha: Univerzita Karlova, 2014, roč.~60, č.~1, s.~90-94. ISSN~0015-5500.

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