DETERMINATION OF TESTOSTERONE AND ITS 5 METABOLITES IN THE
INCUBATION MEDIA AS A MEASURE OF CYTOCHROME P450 METABOLIC
ACTIVITY

D 2014

DETERMINATION OF TESTOSTERONE AND ITS 5 METABOLITES IN THE INCUBATION MEDIA AS A MEASURE OF CYTOCHROME P450 METABOLIC ACTIVITY

TURJAP, Miroslav; Gabriela DOVRTĚLOVÁ; Kristýna NOSKOVÁ; Ondřej ZENDULKA; Jan JUŘICA et al.

Základní údaje

Originální název

DETERMINATION OF TESTOSTERONE AND ITS 5 METABOLITES IN THE INCUBATION MEDIA AS A MEASURE OF CYTOCHROME P450 METABOLIC ACTIVITY

Autoři

TURJAP, Miroslav; Gabriela DOVRTĚLOVÁ; Kristýna NOSKOVÁ; Ondřej ZENDULKA a Jan JUŘICA

Vydání

Brno, CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS, od s. 270-273, 4 s. 2014

Nakladatel

Institute of Analytical Chemistry AS CR

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/14:00082148

Organizační jednotka

Lékařská fakulta

ISBN

978-80-904959-2-0

UT WoS

000354547400088

Klíčová slova anglicky

LIQUID-CHROMATOGRAPHY

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 10. 2015 12:36, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

The innovative HPLC method for the determination of testosterone and its five metabolites in incubation medium with rat liver microsomes is reported. The method utilizes liquid-liquid extraction procedure, with use of internal standard prednisone. Analytes are eluted within 18.7 min. The method enables reliable determination of testosterone, 7 alpha-hydroxytestosterone, 6 beta-hydroxytestosterone, 16 alpha-hydroxytestosterone, 16 beta-hydroxytestosterone, 2 beta-hydroxytestosterone with LOD in the range 1.9-3.9 nmol/L.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

MUNI/A/0886/2013, interní kód MU

Název: Farmakodynamické, farmakokinetické a farmakogenetické přístupy v preklinické fázi výzkumu a vývoje léčiv (Akronym: FDN, FKK a FGEN studie)

Investor: Masarykova univerzita, Farmakodynamické, farmakokinetické a farmakogenetické přístupy v preklinické fázi výzkumu a vývoje léčiv, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

TURJAP, Miroslav; Gabriela DOVRTĚLOVÁ; Kristýna NOSKOVÁ; Ondřej ZENDULKA a Jan JUŘICA. DETERMINATION OF TESTOSTERONE AND ITS 5 METABOLITES IN THE INCUBATION MEDIA AS A MEASURE OF CYTOCHROME P450 METABOLIC ACTIVITY. Online. In František Foret, Jana Křenková, Iveta Drobníková, Andras Guttman, Karel Klepárník. CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS. Brno: Institute of Analytical Chemistry AS CR, 2014, s. 270-273. ISBN 978-80-904959-2-0.

@inproceedings{1204439,
   author = {Turjap, Miroslav and Dovrtělová, Gabriela and Nosková, Kristýna and Zendulka, Ondřej and Juřica, Jan},
   address = {Brno},
   booktitle = {CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS},
   editor = {František Foret, Jana Křenková, Iveta Drobníková, Andras Guttman, Karel Klepárník},
   keywords = {LIQUID-CHROMATOGRAPHY},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-904959-2-0},
   pages = {270-273},
   publisher = {Institute of Analytical Chemistry AS CR},
   title = {DETERMINATION OF TESTOSTERONE AND ITS 5 METABOLITES IN THE INCUBATION MEDIA AS A MEASURE OF CYTOCHROME P450 METABOLIC ACTIVITY},
   year = {2014}
}

TY  - CONF
ID  - 1204439
AU  - Turjap, Miroslav - Dovrtělová, Gabriela - Nosková, Kristýna - Zendulka, Ondřej - Juřica, Jan
PY  - 2014
TI  - DETERMINATION OF TESTOSTERONE AND ITS 5 METABOLITES IN THE INCUBATION MEDIA AS A MEASURE OF CYTOCHROME P450 METABOLIC ACTIVITY
PB  - Institute of Analytical Chemistry AS CR
CY  - Brno
SN  - 9788090495920
KW  - LIQUID-CHROMATOGRAPHY
N2  - The innovative HPLC method for the determination of testosterone and its five metabolites in incubation medium with rat liver microsomes is reported. The method utilizes liquid-liquid extraction procedure, with use of internal standard prednisone. Analytes are eluted within 18.7 min. The method enables reliable determination of testosterone, 7 alpha-hydroxytestosterone, 6 beta-hydroxytestosterone, 16 alpha-hydroxytestosterone, 16 beta-hydroxytestosterone, 2 beta-hydroxytestosterone with LOD in the range 1.9-3.9 nmol/L.
ER  -

TURJAP, Miroslav; Gabriela DOVRTĚLOVÁ; Kristýna NOSKOVÁ; Ondřej ZENDULKA a Jan JUŘICA. DETERMINATION OF TESTOSTERONE AND ITS 5 METABOLITES IN THE INCUBATION MEDIA AS A MEASURE OF CYTOCHROME P450 METABOLIC ACTIVITY. Online. In František Foret, Jana Křenková, Iveta Drobníková, Andras Guttman, Karel Klepárník. \textit{CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS}. Brno: Institute of Analytical Chemistry AS CR, 2014, s.~270-273. ISBN~978-80-904959-2-0.

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