HPLC-MS Identification of Metabolites of Selected
Benzophenanthridine Alkaloids by Rat Liver Microsomes

D 2014

HPLC-MS Identification of Metabolites of Selected Benzophenanthridine Alkaloids by Rat Liver Microsomes

MIDLIK, Adam; Kristýna ŠEBRLOVÁ; Ivana KUŠNYEROVÁ; Gabriela DOVRTĚLOVÁ; Kristýna NOSKOVÁ et al.

Základní údaje

Originální název

HPLC-MS Identification of Metabolites of Selected Benzophenanthridine Alkaloids by Rat Liver Microsomes

Autoři

MIDLIK, Adam; Kristýna ŠEBRLOVÁ; Ivana KUŠNYEROVÁ; Gabriela DOVRTĚLOVÁ; Kristýna NOSKOVÁ; Jan JUŘICA; Eva TÁBORSKÁ a Ondřej PEŠ

Vydání

Praha, Book of Abstracts European Symposium on Atomic Spectrometry ESAS 2014 & 15th Czech - Slovak Spectroscopic Conference, s. 205-474, 2014

Nakladatel

Ioannes Marcus Marci Spectroscopic Society

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

paměťový nosič (CD, DVD, flash disk)

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

ISBN

978-80-905704-1-2

Klíčová slova anglicky

LC MS; benzophenanthridine alkaloids; metabolites; microsomes

Změněno: 7. 11. 2014 08:22, doc. Mgr. Ondřej Peš, Ph.D.

Anotace

V originále

Quaternary benzophenanthridine alkaloids (QBAs) are a group of isoquinoline derivatives produced by plants of the Papaveraceae, Fumariaceae and Rutaceae family. Presently they are studied intensively because of their biological activity. Anti-inflammatory, anti-microbial, anti-fungal, antiparasitic, as well as pro-apoptotic and anti-tumor effects of QBAs have been reported. Determination of toxicity of these potential drugs is based on metabolic studies of major, commercially available sanguinarine (SA) and chelerythrine (CHE). However, metabolic fate and pharmacological activity of minor QBAs, such as chelilutine (CL), chelirubine (CR), sanguilutine (SL), sanguirubine (SR) and macarpine (MA), might differ considerably, despite their structural similarity. Rat liver microsomes (RLMs), subcellular particles containing metabolizing enzymes, such as a cytochrome P450 family, provide a simple metabolic model for in vitro investigation before carrying out in vivo tests.

Návaznosti

LH12176, projekt VaV

Název: Benzofenanthridinové alkaloidy - studium účinků na celulární a molekulární úrovni (Akronym: KBF)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Benzofenathridinové alkaloidy-studium účinků na celulární a molekulární úrovni

MUNI/A/0818/2012, interní kód MU

Název: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Akronym: BIOMET 2)

Investor: Masarykova univerzita, Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

MIDLIK, Adam; Kristýna ŠEBRLOVÁ; Ivana KUŠNYEROVÁ; Gabriela DOVRTĚLOVÁ; Kristýna NOSKOVÁ; Jan JUŘICA; Eva TÁBORSKÁ a Ondřej PEŠ. HPLC-MS Identification of Metabolites of Selected Benzophenanthridine Alkaloids by Rat Liver Microsomes. In Vítězslav Otruba, Michaela Vašinová Galiová. Book of Abstracts European Symposium on Atomic Spectrometry ESAS 2014 & 15th Czech - Slovak Spectroscopic Conference. Praha: Ioannes Marcus Marci Spectroscopic Society, 2014, s. 205-474. ISBN 978-80-905704-1-2.

@inproceedings{1204445,
   author = {Midlik, Adam and Šebrlová, Kristýna and Kušnyerová, Ivana and Dovrtělová, Gabriela and Nosková, Kristýna and Juřica, Jan and Táborská, Eva and Peš, Ondřej},
   address = {Praha},
   booktitle = {Book of Abstracts European Symposium on Atomic Spectrometry ESAS 2014 & 15th Czech - Slovak Spectroscopic Conference},
   editor = {Vítězslav Otruba, Michaela Vašinová Galiová},
   keywords = {LC MS; benzophenanthridine alkaloids; metabolites; microsomes},
   howpublished = {paměťový nosič},
   language = {eng},
   location = {Praha},
   isbn = {978-80-905704-1-2},
   pages = {205-474},
   publisher = {Ioannes Marcus Marci Spectroscopic Society},
   title = {HPLC-MS Identification of Metabolites of Selected Benzophenanthridine Alkaloids by Rat Liver Microsomes},
   year = {2014}
}

TY  - CONF
ID  - 1204445
AU  - Midlik, Adam - Šebrlová, Kristýna - Kušnyerová, Ivana - Dovrtělová, Gabriela - Nosková, Kristýna - Juřica, Jan - Táborská, Eva - Peš, Ondřej
PY  - 2014
TI  - HPLC-MS Identification of Metabolites of Selected Benzophenanthridine Alkaloids by Rat Liver Microsomes
PB  - Ioannes Marcus Marci Spectroscopic Society
CY  - Praha
SN  - 9788090570412
KW  - LC MS
KW  - benzophenanthridine alkaloids
KW  - metabolites
KW  - microsomes
N2  - Quaternary benzophenanthridine alkaloids (QBAs) are a group of isoquinoline derivatives produced by plants of the Papaveraceae, Fumariaceae and Rutaceae family. Presently they are studied intensively because of their biological activity. Anti-inflammatory, anti-microbial, anti-fungal, antiparasitic, as well as pro-apoptotic and anti-tumor effects of QBAs have been reported. Determination of toxicity of these potential drugs is based on metabolic studies of major, commercially available sanguinarine (SA) and chelerythrine (CHE). However, metabolic fate and pharmacological activity of minor QBAs, such as chelilutine (CL), chelirubine (CR), sanguilutine (SL), sanguirubine (SR) and macarpine (MA), might differ considerably, despite their structural similarity. Rat liver microsomes (RLMs), subcellular particles containing metabolizing enzymes, such as a cytochrome P450 family, provide a simple metabolic model for in vitro investigation before carrying out in vivo tests.
ER  -

MIDLIK, Adam; Kristýna ŠEBRLOVÁ; Ivana KUŠNYEROVÁ; Gabriela DOVRTĚLOVÁ; Kristýna NOSKOVÁ; Jan JUŘICA; Eva TÁBORSKÁ a Ondřej PEŠ. HPLC-MS Identification of Metabolites of Selected Benzophenanthridine Alkaloids by Rat Liver Microsomes. In Vítězslav Otruba, Michaela Vašinová Galiová. \textit{Book of Abstracts European Symposium on Atomic Spectrometry ESAS 2014 \&{} 15th Czech - Slovak Spectroscopic Conference}. Praha: Ioannes Marcus Marci Spectroscopic Society, 2014, s.~205-474. ISBN~978-80-905704-1-2.

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