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@article{1204941,
author = {Šimara, Pavel and Tesařová, Lenka and Paďourová, Stanislava and Krontorád Koutná, Irena},
article_location = {Praha},
article_number = {S1},
keywords = {hiPSCs; lentivirus; Sendai virus; episomal reprogramming},
language = {eng},
issn = {0015-5500},
journal = {Folia Biologica},
title = {Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods},
volume = {60},
year = {2014}
}
TY - JOUR
ID - 1204941
AU - Šimara, Pavel - Tesařová, Lenka - Paďourová, Stanislava - Krontorád Koutná, Irena
PY - 2014
TI - Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods
JF - Folia Biologica
VL - 60
IS - S1
SP - 85-89
EP - 85-89
PB - Univerzita Karlova
SN - 00155500
KW - hiPSCs
KW - lentivirus
KW - Sendai virus
KW - episomal reprogramming
N2 - Preclinical studies have demonstrated the promising potential of human induced pluripotent stem cells (hiPSCs) for clinical application. To fulfil this goal, efficient and safe methods to generate them must be established. Various reprogramming techniques were presented during seven years of hiPSCs research. Genome non-integrating and completely xeno-free protocols from the first biopsy to stable hiPSC clones are highly preferable in terms of future clinical application. In this commentary article, we summarize the reprogramming experiments performed in our laboratories. We successfully generated hiPSCs using STEMCCA lentivirus, Sendai virus or episomal vectors. Human neonatal fibroblasts and CD34+ blood progenitors were used as cell sources and were maintained either on mouse embryonic feeder cells or in feeder-free conditions. The reprogramming efficiency was comparable for all three methods and both cell types, while the best results were obtained in feeder-free conditions.
ER -
ŠIMARA, Pavel, Lenka TESAŘOVÁ, Stanislava PAĎOUROVÁ a Irena KRONTORÁD KOUTNÁ. Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods. \textit{Folia Biologica}. Praha: Univerzita Karlova, roč.~60, S1, s.~85-89. ISSN~0015-5500. 2014.
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