2014
Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods
ŠIMARA, Pavel; Lenka TESAŘOVÁ; Stanislava PAĎOUROVÁ a Irena KRONTORÁD KOUTNÁZákladní údaje
Originální název
Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods
Autoři
ŠIMARA, Pavel (203 Česká republika, domácí); Lenka TESAŘOVÁ (203 Česká republika, domácí); Stanislava PAĎOUROVÁ (703 Slovensko, domácí) a Irena KRONTORÁD KOUTNÁ (203 Česká republika, garant)
Vydání
Advances in Molecular and Cancer Biology, 2014
Další údaje
Jazyk
angličtina
Typ výsledku
Prezentace na konferencích
Obor
10601 Cell biology
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14330/14:00074082
Organizační jednotka
Fakulta informatiky
Klíčová slova anglicky
hiPSCs; lentivirus; Sendai virus; episomal reprogramming
Změněno: 2. 3. 2018 10:03, Mgr. Pavel Šimara, Ph.D.
Anotace
V originále
Preclinical studies have demonstrated the promising potential of human induced pluripotent stem cells (hiPSCs) for clinical application. To fulfill this goal, efficient and safe methods to generate them must be established. Various reprogramming techniques were presented during 7 years of hiPSCs research. Genome non-integrating and completely xeno-free protocols from the first biopsy to stable hiPSCs clones are highly preferable in terms of future clinical application. We successfully generated hiPSCs using STEMCCA lentivirus, Sendai virus or episomal vectors. Human fibroblasts and CD34+ blood progenitors were used as a source cells and were maintained either on mouse embryonic feeder cells or in feeder-free conditions. The reprogramming efficiency was comparable for all three methods and both cell types, while the best results were obtained in feeder-free conditions.
Návaznosti
| CZ.1.07/2.3.00/30.0030, interní kód MU |
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| GBP302/12/G157, projekt VaV |
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