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@article{1209991,
author = {Hansel, Robert and Luh, Laura M. and Corberski, Ivan and Trantírek, Lukáš and Dotsch, Volker},
article_location = {Weinheim, Germany},
article_number = {39},
doi = {http://dx.doi.org/10.1002/anie.201311320},
keywords = {MAGNETIC-RESONANCE-SPECTROSCOPY; XENOPUS-LAEVIS OOCYTES; ELECTRON-PARAMAGNETIC-RESONANCE; PROTEIN-PROTEIN INTERACTIONS; HUMAN TELOMERIC DNA; DISTANCE MEASUREMENTS; ESCHERICHIA-COLI; LIVING CELLS; F-19 NMR; PHYSIOLOGICAL CONDITIONS},
language = {eng},
issn = {1433-7851},
journal = {Angewandte Chemie International Edition},
title = {In-Cell NMR and EPR Spectroscopy of Biomacromolecules},
url = {http://onlinelibrary.wiley.com/doi/10.1002/anie.201311320/abstract},
volume = {53},
year = {2014}
}
TY - JOUR
ID - 1209991
AU - Hansel, Robert - Luh, Laura M. - Corberski, Ivan - Trantírek, Lukáš - Dotsch, Volker
PY - 2014
TI - In-Cell NMR and EPR Spectroscopy of Biomacromolecules
JF - Angewandte Chemie International Edition
VL - 53
IS - 39
SP - 10300-10314
EP - 10300-10314
PB - Wiley-VCH Verlag GmbH & Co.
SN - 14337851
KW - MAGNETIC-RESONANCE-SPECTROSCOPY
KW - XENOPUS-LAEVIS OOCYTES
KW - ELECTRON-PARAMAGNETIC-RESONANCE
KW - PROTEIN-PROTEIN INTERACTIONS
KW - HUMAN TELOMERIC DNA
KW - DISTANCE MEASUREMENTS
KW - ESCHERICHIA-COLI
KW - LIVING CELLS
KW - F-19 NMR
KW - PHYSIOLOGICAL CONDITIONS
UR - http://onlinelibrary.wiley.com/doi/10.1002/anie.201311320/abstract
L2 - http://onlinelibrary.wiley.com/doi/10.1002/anie.201311320/abstract
N2 - The dream of cell biologists is to be able to watch biological macromolecules perform their duties in the intracellular environment of live cells. Ideally, the observation of both the location and the conformation of these macromolecules with biophysical techniques is desired. The development of many fluorescence techniques, including super-resolution fluorescence microscopy, has significantly enhanced our ability to spot proteins and other molecules in the crowded cellular environment. However, the observation of their structure and conformational changes while they attend their business is still very challenging. In principle, NMR and EPR spectroscopy can be used to investigate the conformation and dynamics of biological macromolecules in living cells. The development of in-cell magnetic resonance techniques has demonstrated the feasibility of this approach. Herein we review the different techniques with a focus on liquid-state in-cell NMR spectroscopy, provide an overview of applications, and discuss the challenges that lie ahead.
ER -
HANSEL, Robert, Laura M. LUH, Ivan CORBERSKI, Lukáš TRANTÍREK and Volker DOTSCH. In-Cell NMR and EPR Spectroscopy of Biomacromolecules. \textit{Angewandte Chemie International Edition}. Weinheim, Germany: Wiley-VCH Verlag GmbH \&{} Co., 2014, vol.~53, No~39, p.~10300-10314. ISSN~1433-7851. Available from: https://dx.doi.org/10.1002/anie.201311320.
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