TOWARDS CYTOCHROME P450 IMER FOR KINETICS AND INHIBITION
STUDIES USING CAPILLARY ELECTROPHORESIS IN ONLINE CONFIGURATION

D 2014

TOWARDS CYTOCHROME P450 IMER FOR KINETICS AND INHIBITION STUDIES USING CAPILLARY ELECTROPHORESIS IN ONLINE CONFIGURATION

SCHEJBAL, Jan; Roman ŘEMÍNEK and Zdeněk GLATZ

Basic information

Original name

TOWARDS CYTOCHROME P450 IMER FOR KINETICS AND INHIBITION STUDIES USING CAPILLARY ELECTROPHORESIS IN ONLINE CONFIGURATION

Name in Czech

Tvorba imobilizovaného enzymatického mikroreaktoru pro kinetické a inhibiční studie cytochromu P450 s využitím kapilární elektroforézy v on-line uspořádání

Authors

SCHEJBAL, Jan; Roman ŘEMÍNEK and Zdeněk GLATZ

Edition

Brno, 11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014, p. 103-106, 4 pp. 2014

Publisher

Institute of Analytical Chemistry AS CR, v. v. i., Brno, Česká republika

Other information

Language

English

Type of outcome

Proceedings paper

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

Publication form

storage medium (CD, DVD, flash disk)

RIV identification code

RIV/00216224:14740/14:00074138

Organization unit

Central European Institute of Technology

ISBN

978-80-904959-2-0

UT WoS

000354547400036

Keywords (in Czech)

Capillary electrophoresis; Drug metabolism; Cytochrome P450 2C9; IMER; Diclofenac; Sulfaphenazole; Kineticts; Inhbition;

Keywords in English

kapilární elektroforéza; metabolismus léčiv; cytochrom P450 2C9; IMER; diclofenac; sulfafenazol; kinetika; inhibice;

Abstract

In the original language

The enzyme superfamily of cytochromes P450 (CYP) plays a key role in xenobiotic biotransformation and thusly in drug metabolism. Therefore assessment of the interaction between candidate drugs and CYP isoforms is an integral part of preclinical stage of the drug development process, where high throughput and cost effectiveness is a priority. Capillary electrophoresis (CE) offers a number of features which are suitable for these purposes, those being the low sample consumption, short analysis time and parallel analyses using multicapillary systems, what more integration of immobilized enzyme microreactor (IMER) into the separation capillary enables full automation and repeated use of the enzyme. A new online method for inhibition studies based on cytochrome P450 2C9 isoform IMER integrated into CE system is thus presented.

Links

EE2.3.20.0182, research and development project

Name: Vytvoření multidisciplinárního výzkumného a vzdělávacího centra bioanalytických technologií

GAP206/10/0057, research and development project

Name: Miniaturizovaný systém pro "on-line" studie metabolismu léčiv založený na kapilární elektroforéze

Investor: Czech Science Foundation, Miniaturized on-line drug metabolism system based on capillary electrophoresis

Cite

SCHEJBAL, Jan; Roman ŘEMÍNEK and Zdeněk GLATZ. TOWARDS CYTOCHROME P450 IMER FOR KINETICS AND INHIBITION STUDIES USING CAPILLARY ELECTROPHORESIS IN ONLINE CONFIGURATION. In F.Foret. 11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014. Brno: Institute of Analytical Chemistry AS CR, v. v. i., Brno, Česká republika, 2014, p. 103-106. ISBN 978-80-904959-2-0.

@inproceedings{1210572,
   author = {Schejbal, Jan and Řemínek, Roman and Glatz, Zdeněk},
   address = {Brno},
   booktitle = {11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014},
   editor = {F.Foret},
   keywords = {kapilární elektroforéza; metabolismus léčiv; cytochrom P450 2C9; IMER; diclofenac; sulfafenazol; kinetika; inhibice;},
   howpublished = {paměťový nosič},
   language = {eng},
   location = {Brno},
   isbn = {978-80-904959-2-0},
   pages = {103-106},
   publisher = {Institute of Analytical Chemistry AS CR, v. v. i., Brno, Česká republika},
   title = {TOWARDS CYTOCHROME P450 IMER FOR KINETICS AND INHIBITION STUDIES USING CAPILLARY ELECTROPHORESIS IN ONLINE CONFIGURATION},
   year = {2014}
}

TY  - CONF
ID  - 1210572
AU  - Schejbal, Jan - Řemínek, Roman - Glatz, Zdeněk
PY  - 2014
TI  - TOWARDS CYTOCHROME P450 IMER FOR KINETICS AND INHIBITION STUDIES USING CAPILLARY ELECTROPHORESIS IN ONLINE CONFIGURATION
PB  - Institute of Analytical Chemistry AS CR, v. v. i., Brno, Česká republika
CY  - Brno
SN  - 9788090495920
KW  - kapilární elektroforéza
KW  - metabolismus léčiv
KW  - cytochrom P450 2C9
KW  - IMER
KW  - diclofenac
KW  - sulfafenazol
KW  - kinetika
KW  - inhibice;
N2  - The enzyme superfamily of cytochromes P450 (CYP) plays a key role in xenobiotic biotransformation and thusly in drug metabolism. Therefore assessment of the interaction between candidate drugs and CYP isoforms is an integral part of preclinical stage of the drug development process, where high throughput and cost effectiveness is a priority. Capillary electrophoresis (CE) offers a number of features which are suitable for these purposes, those being the low sample consumption, short analysis time and parallel analyses using multicapillary systems, what more integration of immobilized enzyme microreactor (IMER) into the separation capillary enables full automation and repeated use of the enzyme. A new online method for inhibition studies based on cytochrome P450 2C9 isoform IMER integrated into CE system is thus presented.
ER  -

SCHEJBAL, Jan; Roman ŘEMÍNEK and Zdeněk GLATZ. TOWARDS CYTOCHROME P450 IMER FOR KINETICS AND INHIBITION STUDIES USING CAPILLARY ELECTROPHORESIS IN ONLINE CONFIGURATION. In F.Foret. \textit{11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014}. Brno: Institute of Analytical Chemistry AS CR, v. v. i., Brno, Česká republika, 2014, p.~103-106. ISBN~978-80-904959-2-0.

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