A photobioreactor system for precision cultivation of
photoautotrophic microorganisms and for high-content analysis
of suspension dynamics

J 2008

A photobioreactor system for precision cultivation of photoautotrophic microorganisms and for high-content analysis of suspension dynamics

NEDBAL, Ladislav; Milan TRTÍLEK; Jan ČERVENÝ; O KOMAREK; HB PAKRASI et al.

Základní údaje

Originální název

A photobioreactor system for precision cultivation of photoautotrophic microorganisms and for high-content analysis of suspension dynamics

Autoři

NEDBAL, Ladislav; Milan TRTÍLEK; Jan ČERVENÝ; O KOMAREK a HB PAKRASI

Vydání

HOBOKEN, JOHN WILEY & SONS INC, 2008

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

DOI

https://doi.org/10.1002/bit.21833

Klíčová slova anglicky

algae; cyanobacteria; high-content monitoring; microbial growth; photosynthesis; systems biology

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 11. 2014 13:57, Ing. Jan Červený, Ph.D.

Anotace

V originále

Small-scale photobioreactors for cultivation of photoautotrophic microbes are required for precise characterization of the growth parameters of wild-type and engineered strains of these organisms, for their screening, and for optimization of culture conditions. Here, we describe the design and use of a flat-cuvette photobioreactor that allows accurate control of culture irradiance, temperature, PH, and gas composition combined with real-time monitoring by a built-in fluorometer and densitometer. The high-power LED light source generates precise irradiance levels that are programmed by user-designed protocols. The irradiance, temperature, and gas composition may be static or dynamically modulated, while optical density and PH may be stabilized in turbidostat and pH-stat modes, respectively. We demonstrate that the instrument is able to detect minute variations of growth caused, for example, by sudden dilution or by circadian rhythms. The sensitivity of the instrument is sufficient to monitor suspension optical density as low as 10(-2). This newly designed photobioreactor can significantly contribute to the study and use of photoautotrophic microbes in systems biology and biotechnology.

Citovat

NEDBAL, Ladislav; Milan TRTÍLEK; Jan ČERVENÝ; O KOMAREK a HB PAKRASI. A photobioreactor system for precision cultivation of photoautotrophic microorganisms and for high-content analysis of suspension dynamics. HOBOKEN: JOHN WILEY & SONS INC, 2008. Dostupné z: https://doi.org/10.1002/bit.21833.

@article{1210753,
   author = {Nedbal, Ladislav and Trtílek, Milan and Červený, Jan and Komarek, O and Pakrasi, HB},
   article_location = {HOBOKEN},
   doi = {https://doi.org/10.1002/bit.21833},
   keywords = {algae; cyanobacteria; high-content monitoring; microbial growth; photosynthesis; systems biology},
   language = {eng},
   title = {A photobioreactor system for precision cultivation of photoautotrophic microorganisms and for high-content analysis of suspension dynamics},
   year = {2008}
}

TY  - JOUR
ID  - 1210753
AU  - Nedbal, Ladislav - Trtílek, Milan - Červený, Jan - Komarek, O - Pakrasi, HB
PY  - 2008
TI  - A photobioreactor system for precision cultivation of photoautotrophic microorganisms and for high-content analysis of suspension dynamics
PB  - JOHN WILEY & SONS INC
KW  - algae
KW  - cyanobacteria
KW  - high-content monitoring
KW  - microbial growth
KW  - photosynthesis
KW  - systems biology
N2  - Small-scale photobioreactors for cultivation of photoautotrophic microbes are required for precise characterization of the growth parameters of wild-type and engineered strains of these organisms, for their screening, and for optimization of culture conditions. Here, we describe the design and use of a flat-cuvette photobioreactor that allows accurate control of culture irradiance, temperature, PH, and gas composition combined with real-time monitoring by a built-in fluorometer and densitometer. The high-power LED light source generates precise irradiance levels that are programmed by user-designed protocols. The irradiance, temperature, and gas composition may be static or dynamically modulated, while optical density and PH may be stabilized in turbidostat and pH-stat modes, respectively. We demonstrate that the instrument is able to detect minute variations of growth caused, for example, by sudden dilution or by circadian rhythms. The sensitivity of the instrument is sufficient to monitor suspension optical density as low as 10(-2). This newly designed photobioreactor can significantly contribute to the study and use of photoautotrophic microbes in systems biology and biotechnology.
ER  -

NEDBAL, Ladislav; Milan TRTÍLEK; Jan ČERVENÝ; O KOMAREK a HB PAKRASI. A photobioreactor system for precision cultivation of photoautotrophic microorganisms and for high-content analysis of suspension dynamics. HOBOKEN: JOHN WILEY \&{}amp; SONS INC, 2008. Dostupné z: https://doi.org/10.1002/bit.21833.

Přehled o publikaci