Whole Genome Sequence of the Treponema pallidum subsp.
endemicum Strain Bosnia A: The Genome Is Related to Yaws
Treponemes but Contains Few Loci Similar to Syphilis Treponemes

J 2014

Whole Genome Sequence of the Treponema pallidum subsp. endemicum Strain Bosnia A: The Genome Is Related to Yaws Treponemes but Contains Few Loci Similar to Syphilis Treponemes

ŠTAUDOVÁ, Barbora, Michal STROUHAL, Marie ZOBANÍKOVÁ, Darina ČEJKOVÁ, Lucinda L. FULTON et. al.

Základní údaje

Originální název

Whole Genome Sequence of the Treponema pallidum subsp. endemicum Strain Bosnia A: The Genome Is Related to Yaws Treponemes but Contains Few Loci Similar to Syphilis Treponemes

Autoři

ŠTAUDOVÁ, Barbora (203 Česká republika, domácí), Michal STROUHAL (203 Česká republika, domácí), Marie ZOBANÍKOVÁ (203 Česká republika, domácí), Darina ČEJKOVÁ (203 Česká republika, domácí), Lucinda L. FULTON (840 Spojené státy), Lei CHEN (840 Spojené státy), lorenzo GIACANI (840 Spojené státy), Arturo CENTURION-LARA (840 Spojené státy), Sylvia M. BRUISTEN (528 Nizozemské království), Erica SODERGREN (840 Spojené státy), George M. WEINSTOCK (840 Spojené státy) a David ŠMAJS (203 Česká republika, garant, domácí)

Vydání

PLoS neglected tropical diseases, San Francisco, Public Library of Science, 2014, 1935-2735

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.446

Kód RIV

RIV/00216224:14110/14:00074160

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1371/journal.pntd.0003261

UT WoS

000345514000016

Klíčová slova anglicky

Treponema pallidum; endemic syphilis

Štítky

EL OK

Příznaky

Recenzováno

Změněno: 31. 1. 2015 23:46, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Background: T. pallidum subsp. endemicum (TEN) is the causative agent of bejel (also known as endemic syphilis). Clinical symptoms of syphilis and bejel are overlapping and the epidemiological context is important for correct diagnosis of both diseases. In contrast to syphilis, caused by T. pallidum subsp. pallidum (TPA), TEN infections are usually spread by direct contact or contaminated utensils rather than by sexual contact. Bejel is most often seen in western Africa and in the Middle East. The strain Bosnia A was isolated in 1950 in Bosnia, southern Europe. Methodology/Principal Findings: The complete genome of the Bosnia A strain was amplified and sequenced using the pooled segment genome sequencing (PSGS) method and a combination of three next-generation sequencing techniques (SOLiD, Roche 454, and Illumina). Using this approach, a total combined average genome coverage of 5136was achieved. The size of the Bosnia A genome was found to be 1,137,653 bp, i.e. 1.6–2.8 kbp shorter than any previously published genomes of uncultivable pathogenic treponemes. Conserved gene synteny was found in the Bosnia A genome compared to other sequenced syphilis and yaws treponemes. The TEN Bosnia A genome was distinct but very similar to the genome of yaws-causing T. pallidum subsp. pertenue (TPE) strains. Interestingly, the TEN Bosnia A genome was found to contain several sequences, which so far, have been uniquely identified only in syphilis treponemes. Conclusions/Significance: The genome of TEN Bosnia A contains several sequences thought to be unique to TPA strains; these sequences very likely represent remnants of recombination events during the evolution of TEN treponemes. This finding emphasizes a possible role of repeated horizontal gene transfer between treponemal subspecies in shaping the Bosnia A genome.

Návaznosti

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GAP302/12/0574, projekt VaV

Název: Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

Investor: Grantová agentura ČR, Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

NT11159, projekt VaV

Název: Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Investor: Ministerstvo zdravotnictví ČR, Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Citovat

ŠTAUDOVÁ, Barbora, Michal STROUHAL, Marie ZOBANÍKOVÁ, Darina ČEJKOVÁ, Lucinda L. FULTON, Lei CHEN, lorenzo GIACANI, Arturo CENTURION-LARA, Sylvia M. BRUISTEN, Erica SODERGREN, George M. WEINSTOCK a David ŠMAJS. Whole Genome Sequence of the Treponema pallidum subsp. endemicum Strain Bosnia A: The Genome Is Related to Yaws Treponemes but Contains Few Loci Similar to Syphilis Treponemes. PLoS neglected tropical diseases. San Francisco: Public Library of Science, 2014, roč. 8, č. 11, s. "e3261", 13 s. ISSN 1935-2735. Dostupné z: https://dx.doi.org/10.1371/journal.pntd.0003261.

@article{1211569,
   author = {Štaudová, Barbora and Strouhal, Michal and Zobaníková, Marie and Čejková, Darina and Fulton, Lucinda L. and Chen, Lei and Giacani, lorenzo and CenturionandLara, Arturo and Bruisten, Sylvia M. and Sodergren, Erica and Weinstock, George M. and Šmajs, David},
   article_location = {San Francisco},
   article_number = {11},
   doi = {http://dx.doi.org/10.1371/journal.pntd.0003261},
   keywords = {Treponema pallidum; endemic syphilis},
   language = {eng},
   issn = {1935-2735},
   journal = {PLoS neglected tropical diseases},
   title = {Whole Genome Sequence of the Treponema pallidum subsp. endemicum Strain Bosnia A: The Genome Is Related to Yaws Treponemes but Contains Few Loci Similar to Syphilis Treponemes},
   volume = {8},
   year = {2014}
}

TY  - JOUR
ID  - 1211569
AU  - Štaudová, Barbora - Strouhal, Michal - Zobaníková, Marie - Čejková, Darina - Fulton, Lucinda L. - Chen, Lei - Giacani, lorenzo - Centurion-Lara, Arturo - Bruisten, Sylvia M. - Sodergren, Erica - Weinstock, George M. - Šmajs, David
PY  - 2014
TI  - Whole Genome Sequence of the Treponema pallidum subsp. endemicum Strain Bosnia A: The Genome Is Related to Yaws Treponemes but Contains Few Loci Similar to Syphilis Treponemes
JF  - PLoS neglected tropical diseases
VL  - 8
IS  - 11
SP  - "e3261"
EP  - "e3261"
PB  - Public Library of Science
SN  - 19352735
KW  - Treponema pallidum
KW  - endemic syphilis
N2  - Background: T. pallidum subsp. endemicum (TEN) is the causative agent of bejel (also known as endemic syphilis). Clinical symptoms of syphilis and bejel are overlapping and the epidemiological context is important for correct diagnosis of both diseases. In contrast to syphilis, caused by T. pallidum subsp. pallidum (TPA), TEN infections are usually spread by direct contact or contaminated utensils rather than by sexual contact. Bejel is most often seen in western Africa and in the Middle East. The strain Bosnia A was isolated in 1950 in Bosnia, southern Europe. Methodology/Principal Findings: The complete genome of the Bosnia A strain was amplified and sequenced using the pooled segment genome sequencing (PSGS) method and a combination of three next-generation sequencing techniques (SOLiD, Roche 454, and Illumina). Using this approach, a total combined average genome coverage of 5136was achieved. The size of the Bosnia A genome was found to be 1,137,653 bp, i.e. 1.6–2.8 kbp shorter than any previously published genomes of uncultivable pathogenic treponemes. Conserved gene synteny was found in the Bosnia A genome compared to other sequenced syphilis and yaws treponemes. The TEN Bosnia A genome was distinct but very similar to the genome of yaws-causing T. pallidum subsp. pertenue (TPE) strains. Interestingly, the TEN Bosnia A genome was found to contain several sequences, which so far, have been uniquely identified only in syphilis treponemes. Conclusions/Significance: The genome of TEN Bosnia A contains several sequences thought to be unique to TPA strains; these sequences very likely represent remnants of recombination events during the evolution of TEN treponemes. This finding emphasizes a possible role of repeated horizontal gene transfer between treponemal subspecies in shaping the Bosnia A genome.
ER  -

ŠTAUDOVÁ, Barbora, Michal STROUHAL, Marie ZOBANÍKOVÁ, Darina ČEJKOVÁ, Lucinda L. FULTON, Lei CHEN, lorenzo GIACANI, Arturo CENTURION-LARA, Sylvia M. BRUISTEN, Erica SODERGREN, George M. WEINSTOCK a David ŠMAJS. Whole Genome Sequence of the Treponema pallidum subsp. endemicum Strain Bosnia A: The Genome Is Related to Yaws Treponemes but Contains Few Loci Similar to Syphilis Treponemes. \textit{PLoS neglected tropical diseases}. San Francisco: Public Library of Science, 2014, roč.~8, č.~11, s.~''e3261'', 13 s. ISSN~1935-2735. Dostupné z: https://dx.doi.org/10.1371/journal.pntd.0003261.

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