Improvement in Staphylococcus and Bacillus strain
differentiation by matrix-assisted laser desorption/ionization
time-of-flight mass spectrometry profiling by using
microwave-assisted enzymatic digestion

J 2014

Improvement in Staphylococcus and Bacillus strain differentiation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling by using microwave-assisted enzymatic digestion

BALÁŽOVÁ, Tereza, Ondrej ŠEDO, Polonca ŠTEFANIC, Ines MANDIC-MULEC, Michiel VOS et. al.

Basic information

Original name

Improvement in Staphylococcus and Bacillus strain differentiation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling by using microwave-assisted enzymatic digestion

Authors

BALÁŽOVÁ, Tereza (203 Czech Republic, belonging to the institution), Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Polonca ŠTEFANIC (705 Slovenia), Ines MANDIC-MULEC (705 Slovenia), Michiel VOS (826 United Kingdom of Great Britain and Northern Ireland) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Rapid Communications in Mass Spectrometry, Hoboken (USA), Wiley-Blackwell, 2014, 0951-4198

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.253

RIV identification code

RIV/00216224:14740/14:00077626

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1002/rcm.6966

UT WoS

000340452100002

Keywords in English

proteomics; bacteria; identification; bacillus subtilis

Abstract

V originále

RATIONALE: Distinguishing between individual bacterial strains below the species level is a challenge to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) bacterial profiling. We propose a quick method for improving strain differentiation of two Staphylococcus and one Bacillus species. METHODS: An alternative procedure to the extraction protocol recommended by Bruker Daltonics was developed. Ethanol-sterilized cells of six S. aureus and six S. haemolyticus strains were digested by trypsin using 2-min microwave irradiation and were then analyzed. Twenty-eight strains belonging to two ecotypes of B. subtilis were subjected to the same procedure to extend the scope of the method. RESULTS: S. aureus and S. haemolyticus strains, only partially distinguishable by the standard sample preparation procedure, were subjected to microwave-assisted tryptic digestion. The repeatability of the procedure was checked in three experiments accomplished at weekly intervals. Clear distinction of the strains was achieved by cluster analysis. The differentiation of B. subtilis ecotypes was also improved significantly by the digestion method. The discriminatory power of the novel method was supported by an increase in the number of strain-specific peaks, as compared to the standard method. CONCLUSIONS: The method modulates the discriminatory power of MALDI-TOF MS profiling. The differentiation of a set of S. aureus, S. haemolyticus and B. subtilis strains was improved significantly after microwave-accelerated tryptic digestion of the cellular material.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.20.0183, research and development project

Name: Centrum experimentální biomedicíny

LM2011020, research and development project

Name: CEITEC ? open access

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

BALÁŽOVÁ, Tereza, Ondrej ŠEDO, Polonca ŠTEFANIC, Ines MANDIC-MULEC, Michiel VOS and Zbyněk ZDRÁHAL. Improvement in Staphylococcus and Bacillus strain differentiation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling by using microwave-assisted enzymatic digestion. Rapid Communications in Mass Spectrometry. Hoboken (USA): Wiley-Blackwell, 2014, vol. 28, No 17, p. 1855-1861. ISSN 0951-4198. Available from: https://dx.doi.org/10.1002/rcm.6966.

@article{1211680,
   author = {Balážová, Tereza and Šedo, Ondrej and Štefanic, Polonca and MandicandMulec, Ines and Vos, Michiel and Zdráhal, Zbyněk},
   article_location = {Hoboken (USA)},
   article_number = {17},
   doi = {http://dx.doi.org/10.1002/rcm.6966},
   keywords = {proteomics; bacteria; identification; bacillus subtilis},
   language = {eng},
   issn = {0951-4198},
   journal = {Rapid Communications in Mass Spectrometry},
   title = {Improvement in Staphylococcus and Bacillus strain differentiation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling by using microwave-assisted enzymatic digestion},
   url = {http://onlinelibrary.wiley.com.ezproxy.muni.cz/doi/10.1002/rcm.6966/abstract;jsessionid=3C620400539588CB37DB7BEF515359BD.f01t01},
   volume = {28},
   year = {2014}
}

TY  - JOUR
ID  - 1211680
AU  - Balážová, Tereza - Šedo, Ondrej - Štefanic, Polonca - Mandic-Mulec, Ines - Vos, Michiel - Zdráhal, Zbyněk
PY  - 2014
TI  - Improvement in Staphylococcus and Bacillus strain differentiation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling by using microwave-assisted enzymatic digestion
JF  - Rapid Communications in Mass Spectrometry
VL  - 28
IS  - 17
SP  - 1855-1861
EP  - 1855-1861
PB  - Wiley-Blackwell
SN  - 09514198
KW  - proteomics
KW  - bacteria
KW  - identification
KW  - bacillus subtilis
UR  - http://onlinelibrary.wiley.com.ezproxy.muni.cz/doi/10.1002/rcm.6966/abstract;jsessionid=3C620400539588CB37DB7BEF515359BD.f01t01
L2  - http://onlinelibrary.wiley.com.ezproxy.muni.cz/doi/10.1002/rcm.6966/abstract;jsessionid=3C620400539588CB37DB7BEF515359BD.f01t01
N2  - RATIONALE: Distinguishing between individual bacterial strains below the species level is a challenge to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) bacterial profiling. We propose a quick method for improving strain differentiation of two Staphylococcus and one Bacillus species. METHODS: An alternative procedure to the extraction protocol recommended by Bruker Daltonics was developed. Ethanol-sterilized cells of six S. aureus and six S. haemolyticus strains were digested by trypsin using 2-min microwave irradiation and were then analyzed. Twenty-eight strains belonging to two ecotypes of B. subtilis were subjected to the same procedure to extend the scope of the method. RESULTS: S. aureus and S. haemolyticus strains, only partially distinguishable by the standard sample preparation procedure, were subjected to microwave-assisted tryptic digestion. The repeatability of the procedure was checked in three experiments accomplished at weekly intervals. Clear distinction of the strains was achieved by cluster analysis. The differentiation of B. subtilis ecotypes was also improved significantly by the digestion method. The discriminatory power of the novel method was supported by an increase in the number of strain-specific peaks, as compared to the standard method. CONCLUSIONS: The method modulates the discriminatory power of MALDI-TOF MS profiling. The differentiation of a set of S. aureus, S. haemolyticus and B. subtilis strains was improved significantly after microwave-accelerated tryptic digestion of the cellular material.
ER  -

BALÁŽOVÁ, Tereza, Ondrej ŠEDO, Polonca ŠTEFANIC, Ines MANDIC-MULEC, Michiel VOS and Zbyněk ZDRÁHAL. Improvement in Staphylococcus and Bacillus strain differentiation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling by using microwave-assisted enzymatic digestion. \textit{Rapid Communications in Mass Spectrometry}. Hoboken (USA): Wiley-Blackwell, 2014, vol.~28, No~17, p.~1855-1861. ISSN~0951-4198. Available from: https://dx.doi.org/10.1002/rcm.6966.

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