Redescription and molecular characterisation of Dujardinascaris madagascariensis and a note on D. dujardini (Nematoda: Heterocheilidae), parasites of Crocodylus niloticus, with a key to Dujardinascaris spp. in crocodilians

MAŠOVÁ, Šárka, Vlastimil BARUŠ, Mária SEIFERTOVÁ, John MALALA and Miloslav JIRKŮ. Redescription and molecular characterisation of Dujardinascaris madagascariensis and a note on D. dujardini (Nematoda: Heterocheilidae), parasites of Crocodylus niloticus, with a key to Dujardinascaris spp. in crocodilians. Zootaxa. New Zealand: Magnolia Press, vol. 3893, No 2, p. 261-276. ISSN 1175-5326. doi:10.11646/zootaxa.3893.2.6. 2014.

Basic information

• Original name: Redescription and molecular characterisation of Dujardinascaris madagascariensis and a note on D. dujardini (Nematoda: Heterocheilidae), parasites of Crocodylus niloticus, with a key to Dujardinascaris spp. in crocodilians
• Authors: MAŠOVÁ, Šárka (203 Czech Republic, guarantor, belonging to the institution), Vlastimil BARUŠ (203 Czech Republic), Mária SEIFERTOVÁ (703 Slovakia, belonging to the institution), John MALALA (404 Kenya) and Miloslav JIRKŮ (203 Czech Republic).
• Edition: Zootaxa, New Zealand, Magnolia Press, 2014, 1175-5326.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: New Zealand
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 0.906
• RIV identification code: RIV/00216224:14310/14:00074171
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.11646/zootaxa.3893.2.6
• UT WoS: 000346874900006
• Keywords (in Czech): jezero Turkana; deltařeky Omo; Afrika; Keňa; bar-coding; 18S rDNA; ITS2
• Keywords in English: Lake Turkana; Omo River delta; Kenya; Africa; bar-coding; 18S rDNA; ITS2
• Tags: Africa, AKR, barcoding, Nematoda, rivok, SEM study, SSU

Abstract

An examination of one specimen of Nile crocodile, Crocodylus niloticus (Laurenti, 1768), from Lake Turkana (Kenya), revealed the presence of two ascaridoid nematodes belonging to the genus Dujardinascaris Baylis, 1947. Dujardinascaris madagascariensis Chabaud & Caballero, 1966 was studied by scanning electron microscopy, redescribed, and differentiated from D. dujardini (Travassos, 1920). Dujardinascaris madagascariencsis is the second of the genus to be sequenced. An internal fragment of the small ribosomal subunit and nuclear ribosomal DNA internal transcribed spacer 2 region were amplified—the slowly evolving 18S gene region was used for phylogenetic analysis. Molecular data confirmed affinity of D. madagascariensis to the family Heterocheilidae and revealed its closest relationship with D. waltoni. A key to the species of Dujardinascaris parasitizing crocodiles is provided.

Links

• GBP505/12/G112, research and development project: Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation