Nonaqueous capillary electrophoresis of dextromethorphan and
its metabolites

J 2014

Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites

PELCOVÁ, Marta; Monika LANGMAJEROVÁ; Eliška CVINGRÁFOVÁ; Jan JUŘICA; Zdeněk GLATZ et. al.

Základní údaje

Originální název

Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites

Autoři

PELCOVÁ, Marta (203 Česká republika, domácí); Monika LANGMAJEROVÁ (203 Česká republika, domácí); Eliška CVINGRÁFOVÁ (203 Česká republika, domácí); Jan JUŘICA (203 Česká republika, domácí) a Zdeněk GLATZ (203 Česká republika, garant, domácí)

Vydání

JOURNAL OF SEPARATION SCIENCE, 2014, 1615-9306

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.737

Kód RIV

RIV/00216224:14310/14:00074172

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1002/jssc.201400582

UT WoS

000342909300021

EID Scopus

2-s2.0-84908076583

Klíčová slova anglicky

Dextromethorphan; Dextrorphan; Methanol; Nonaqueous media

Štítky

AKR, rivok

Změněno: 13. 3. 2018 12:00, doc. PharmDr. Jan Juřica, Ph.D.

Anotace

V originále

This study deals with the nonaqueous capillary electrophoretic separation of dextromethorphan and its metabolites using a methanolic background electrolyte. The optimization of separation conditions was performed in terms of the resolution of dextromethorphan and dextrorphan and the effect of separation temperature, voltage, and the characteristics of the background electrolyte were studied. Complete separation of all analytes was achieved in 40 mM ammonium acetate dissolved in methanol. Hydrodynamic injection was performed at 3 kPa for 4 s. The separation voltage was 20 kV accompanied by a low electric current. The ultraviolet detection was performed at 214 nm, the temperature of the capillary was 25°C. These conditions enabled the separation of four analytes plus the internal standard within 9 min. Further, the developed method was validated in terms of linearity, sensitivity, and repeatability. Rat liver perfusate samples were subjected to the nonaqueous capillary electrophoretic method to illustrate its applicability.

Návaznosti

GBP206/12/G014, projekt VaV

Název: Centrum pokročilých bioanalytických technologií

Citovat

PELCOVÁ, Marta; Monika LANGMAJEROVÁ; Eliška CVINGRÁFOVÁ; Jan JUŘICA a Zdeněk GLATZ. Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites. JOURNAL OF SEPARATION SCIENCE. 2014, roč. 37, č. 19, s. 2785-2790. ISSN 1615-9306. Dostupné z: https://dx.doi.org/10.1002/jssc.201400582.

@article{1212414,
   author = {Pelcová, Marta and Langmajerová, Monika and Cvingráfová, Eliška and Juřica, Jan and Glatz, Zdeněk},
   article_number = {19},
   doi = {http://dx.doi.org/10.1002/jssc.201400582},
   keywords = {Dextromethorphan; Dextrorphan; Methanol; Nonaqueous media},
   language = {eng},
   issn = {1615-9306},
   journal = {JOURNAL OF SEPARATION SCIENCE},
   title = {Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites},
   volume = {37},
   year = {2014}
}

TY  - JOUR
ID  - 1212414
AU  - Pelcová, Marta - Langmajerová, Monika - Cvingráfová, Eliška - Juřica, Jan - Glatz, Zdeněk
PY  - 2014
TI  - Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites
JF  - JOURNAL OF SEPARATION SCIENCE
VL  - 37
IS  - 19
SP  - 2785-2790
EP  - 2785-2790
SN  - 16159306
KW  - Dextromethorphan
KW  - Dextrorphan
KW  - Methanol
KW  - Nonaqueous media
N2  - This study deals with the nonaqueous capillary electrophoretic separation of dextromethorphan and its metabolites using a methanolic background electrolyte. The optimization of separation conditions was performed in terms of the resolution of dextromethorphan and dextrorphan and the effect of separation temperature, voltage, and the characteristics of the background electrolyte were studied. Complete separation of all analytes was achieved in 40 mM ammonium acetate dissolved in methanol. Hydrodynamic injection was performed at 3 kPa for 4 s. The separation voltage was 20 kV accompanied by a low electric current. The ultraviolet detection was performed at 214 nm, the temperature of the capillary was 25°C. These conditions enabled the separation of four analytes plus the internal standard within 9 min. Further, the developed method was validated in terms of linearity, sensitivity, and repeatability. Rat liver perfusate samples were subjected to the nonaqueous capillary electrophoretic method to illustrate its applicability.
ER  -

PELCOVÁ, Marta; Monika LANGMAJEROVÁ; Eliška CVINGRÁFOVÁ; Jan JUŘICA a Zdeněk GLATZ. Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites. \textit{JOURNAL OF SEPARATION SCIENCE}. 2014, roč.~37, č.~19, s.~2785-2790. ISSN~1615-9306. Dostupné z: https://dx.doi.org/10.1002/jssc.201400582.

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