A Pseudomonas putida Strain Genetically Engineered for
1,2,3-Trichloropropane Bioremediation.

J 2014

A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation.

GHUFRANA, S.; Martina PAVLOVÁ; M.I. ARIF; C.P. POSTEMA; Jiří DAMBORSKÝ et. al.

Základní údaje

Originální název

A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation.

Autoři

GHUFRANA, S.; Martina PAVLOVÁ; M.I. ARIF; C.P. POSTEMA; Jiří DAMBORSKÝ a D.B. JANSSEN

Vydání

Applied and Environmental Microbiology, 2014, 0099-2240

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.668

Kód RIV

RIV/00216224:14310/14:00074202

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1128/AEM.01620-14

UT WoS

000341486500032

EID Scopus

2-s2.0-84905904264

Klíčová slova anglicky

1; 2;3-Trichloropropane; biodegradation; Pseudomonas putida MC4

Štítky

AKR, rivok

Změněno: 21. 1. 2015 15:41, Ing. Andrea Mikešková

Anotace

V originále

1,2,3-Trichloropropane (TCP) is a toxic compound that is recalcitrant to biodegradation in the environment and attempts to isolate TCP-degrading organisms using enrichment cultivation have failed. A potential biodegradation pathway starts with hydrolytic dehalogenation to 2,3 dichloro-1-propanol (DCP), followed by oxidative metabolism. To obtain a practically applicable TCP-degrading organism, we introduced an engineered haloalkane dehalogenase with improved TCP degradation activity into the DCP-degrading bacterium Pseudomonas putida MC4. For this, the dehalogenase gene (dhaA31) was cloned behind the constitutive dhlA promoter and introduced into the genome of strain MC4 using a transposon delivery system. The transposon-located antibiotic resistance marker was subsequently removed using a resolvase step. Growth of the resulting engineered bacterium P. putida MC4-5222 on TCP was indeed observed, and all organic chlorine was released as chloride. A packed-bed reactor with immobilized cells of strain MC4 5222 degraded >95% of influent TCP (0.33 mM) under continuous flow conditions, with stoichiometric release of inorganic chloride. The results show the use of a laboratory-evolved dehalogenase and genetic engineering for obtaining an effective plasmid-free and stable whole-cell biocatalyst for the aerobic bioremediation of a recalcitrant chlorinated hydrocarbon.

Návaznosti

GAP503/12/0572, projekt VaV

Název: Konstrukce syntetické metabolické dráhy pro degradaci důležitého environmentálního polutantu proteinovým a metabolickým inženýrstvím

Investor: Grantová agentura ČR, Konstrukce syntetické metabolické dráhy pro degradaci důležitého environmentlního polutantu proteinovým a metabolickým inženýrstvím

Citovat

GHUFRANA, S.; Martina PAVLOVÁ; M.I. ARIF; C.P. POSTEMA; Jiří DAMBORSKÝ a D.B. JANSSEN. A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation. Applied and Environmental Microbiology. 2014, roč. 80, č. 17, s. 5467-5476. ISSN 0099-2240. Dostupné z: https://doi.org/10.1128/AEM.01620-14.

@article{1213426,
   author = {Ghufrana, S. and Pavlová, Martina and Arif, M.I. and Postema, C.P. and Damborský, Jiří and Janssen, D.B.},
   article_number = {17},
   doi = {https://doi.org/10.1128/AEM.01620-14},
   keywords = {1; 2;3-Trichloropropane; biodegradation; Pseudomonas putida MC4},
   language = {eng},
   issn = {0099-2240},
   journal = {Applied and Environmental Microbiology},
   title = {A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation.},
   volume = {80},
   year = {2014}
}

TY  - JOUR
ID  - 1213426
AU  - Ghufrana, S. - Pavlová, Martina - Arif, M.I. - Postema, C.P. - Damborský, Jiří - Janssen, D.B.
PY  - 2014
TI  - A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation.
JF  - Applied and Environmental Microbiology
VL  - 80
IS  - 17
SP  - 5467-5476
EP  - 5467-5476
SN  - 00992240
KW  - 1
KW  - 2;3-Trichloropropane
KW  - biodegradation
KW  - Pseudomonas putida MC4
N2  - 1,2,3-Trichloropropane (TCP) is a toxic compound that is recalcitrant to biodegradation in the environment and attempts to isolate TCP-degrading organisms using enrichment cultivation have failed. A potential biodegradation pathway starts with hydrolytic dehalogenation to 2,3 dichloro-1-propanol (DCP), followed by oxidative metabolism. To obtain a practically applicable TCP-degrading organism, we introduced an engineered haloalkane dehalogenase with improved TCP degradation activity into the DCP-degrading bacterium Pseudomonas putida MC4. For this, the dehalogenase gene (dhaA31) was cloned behind the constitutive dhlA promoter and introduced into the genome of strain MC4 using a transposon delivery system. The transposon-located antibiotic resistance marker was subsequently removed using a resolvase step. Growth of the resulting engineered bacterium P. putida MC4-5222 on TCP was indeed observed, and all organic chlorine was released as chloride. A packed-bed reactor with immobilized cells of strain MC4 5222 degraded >95% of influent TCP (0.33 mM) under continuous flow conditions, with stoichiometric release of inorganic chloride. The results show the use of a laboratory-evolved dehalogenase and genetic engineering for obtaining an effective plasmid-free and stable whole-cell biocatalyst for the aerobic bioremediation of a recalcitrant chlorinated hydrocarbon.
ER  -

GHUFRANA, S.; Martina PAVLOVÁ; M.I. ARIF; C.P. POSTEMA; Jiří DAMBORSKÝ a D.B. JANSSEN. A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation. \textit{Applied and Environmental Microbiology}. 2014, roč.~80, č.~17, s.~5467-5476. ISSN~0099-2240. Dostupné z: https://doi.org/10.1128/AEM.01620-14.

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