| MÁDR, Aleš, Andrea CELÁ, Bořivoj KLEJDUS, Marta PELCOVÁ, Igor CRHA, Jana ŽÁKOVÁ a Zdeněk GLATZ. Determination of pyruvate and lactate as potential biomarkers of embryo viability in assisted reproduction by capillary electrophoresis with contactless conductivity detection. Electrophoresis. 2015, roč. 36, 11-12, s. 1244-1250. ISSN 0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201400487. |
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@article{1214175,
author = {Mádr, Aleš and Celá, Andrea and Klejdus, Bořivoj and Pelcová, Marta and Crha, Igor and Žáková, Jana and Glatz, Zdeněk},
article_number = {11-12},
doi = {http://dx.doi.org/10.1002/elps.201400487},
keywords = {assisted reproduction; capillary electrophoresis; conductivity detection; lactate; pyruvate},
language = {eng},
issn = {0173-0835},
journal = {Electrophoresis},
title = {Determination of pyruvate and lactate as potential biomarkers of embryo viability in assisted reproduction by capillary electrophoresis with contactless conductivity detection},
volume = {36},
year = {2015}
}
TY - JOUR
ID - 1214175
AU - Mádr, Aleš - Celá, Andrea - Klejdus, Bořivoj - Pelcová, Marta - Crha, Igor - Žáková, Jana - Glatz, Zdeněk
PY - 2015
TI - Determination of pyruvate and lactate as potential biomarkers of embryo viability in assisted reproduction by capillary electrophoresis with contactless conductivity detection
JF - Electrophoresis
VL - 36
IS - 11-12
SP - 1244-1250
EP - 1244-1250
SN - 01730835
KW - assisted reproduction
KW - capillary electrophoresis
KW - conductivity detection
KW - lactate
KW - pyruvate
N2 - Importance of human assisted reproduction is rising as a response for rising number of couples suffering from infertility. The key steps in human assisted reproduction are in vitro fertilization of an oocyte, cultivation of embryo and selection of an embryo with the highest developmental potential for implantation. Assessment of developmental potential of embryo is lacking of precision thus novel techniques are sought. Capillary electrophoresis with contactless conductivity detection was applied for analysis of pyruvate and lactate as potential biomarkers of developmental potential of human embryos. Optimizations of separation conditions and treatment of a sample are described and discussed. Developed method was successfully applied for analysis of several samples of spent culture media collected after cultivation of embryos.
ER -
MÁDR, Aleš, Andrea CELÁ, Bořivoj KLEJDUS, Marta PELCOVÁ, Igor CRHA, Jana ŽÁKOVÁ a Zdeněk GLATZ. Determination of pyruvate and lactate as potential biomarkers of embryo viability in assisted reproduction by capillary electrophoresis with contactless conductivity detection. \textit{Electrophoresis}. 2015, roč.~36, 11-12, s.~1244-1250. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201400487.
|
