Rapid Detection of Microorganisms Based on Active and Passive
Modes of QCM

J 2015

Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM

FARKA, Zdeněk; David KOVÁŘ a Petr SKLÁDAL

Základní údaje

Originální název

Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM

Autoři

FARKA, Zdeněk (203 Česká republika, domácí); David KOVÁŘ (203 Česká republika, domácí) a Petr SKLÁDAL (203 Česká republika, garant, domácí)

Vydání

Sensors, Basel, (Switzerland), MDPI AG, 2015, 1424-8220

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.033

Kód RIV

RIV/00216224:14740/15:00082200

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.3390/s150100079

UT WoS

000348309400005

EID Scopus

2-s2.0-84919823099

Klíčová slova anglicky

Escherichia coli; immunosensor; impedance analysis; label-free detection; quartz crystal microbalance

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 9. 2017 11:06, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

Label-free immunosensors are well suited for detection of microorganisms because of their fast response and reasonable sensitivity comparable to infection doses of common pathogens. Active (lever oscillator and frequency counter) and passive (impedance analyzer) modes of quartz crystal microbalance (QCM) were used and compared for rapid detection of three strains of E. coli. Different approaches for antibody immobilization were compared, the immobilization of reduced antibody using Sulfo-SMCC was most effective achieving the limit of detection (LOD) 8 × 10^4 CFU·mL-1 in 10 min. For the passive mode, software evaluating impedance characteristics in real-time was developed and used. Almost the same results were achieved using both active and passive modes confirming that the sensor properties are not limited by the frequency evaluation method but mainly by affinity of the antibody. Furthermore, reference measurements were done using surface plasmon resonance. Effect of condition of cells on signal was observed showing that cells ruptured by ultrasonication provided slightly higher signal changes than intact microbes.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

FARKA, Zdeněk; David KOVÁŘ a Petr SKLÁDAL. Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM. Sensors. Basel, (Switzerland): MDPI AG, 2015, roč. 15, č. 1, s. 79-92. ISSN 1424-8220. Dostupné z: https://doi.org/10.3390/s150100079.

@article{1214396,
   author = {Farka, Zdeněk and Kovář, David and Skládal, Petr},
   article_location = {Basel, (Switzerland)},
   article_number = {1},
   doi = {https://doi.org/10.3390/s150100079},
   keywords = {Escherichia coli; immunosensor; impedance analysis; label-free detection; quartz crystal microbalance},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM},
   url = {http://www.mdpi.com/1424-8220/15/1/79},
   volume = {15},
   year = {2015}
}

TY  - JOUR
ID  - 1214396
AU  - Farka, Zdeněk - Kovář, David - Skládal, Petr
PY  - 2015
TI  - Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM
JF  - Sensors
VL  - 15
IS  - 1
SP  - 79-92
EP  - 79-92
PB  - MDPI AG
SN  - 14248220
KW  - Escherichia coli
KW  - immunosensor
KW  - impedance analysis
KW  - label-free detection
KW  - quartz crystal microbalance
UR  - http://www.mdpi.com/1424-8220/15/1/79
L2  - http://www.mdpi.com/1424-8220/15/1/79
N2  - Label-free immunosensors are well suited for detection of microorganisms because of their fast response and reasonable sensitivity comparable to infection doses of common pathogens. Active (lever oscillator and frequency counter) and passive (impedance analyzer) modes of quartz crystal microbalance (QCM) were used and compared for rapid detection of three strains of E. coli. Different approaches for antibody immobilization were compared, the immobilization of reduced antibody using Sulfo-SMCC was most effective achieving the limit of detection (LOD) 8 × 10^4 CFU·mL-1 in 10 min. For the passive mode, software evaluating impedance characteristics in real-time was developed and used. Almost the same results were achieved using both active and passive modes confirming that the sensor properties are not limited by the frequency evaluation method but mainly by affinity of the antibody. Furthermore, reference measurements were done using surface plasmon resonance. Effect of condition of cells on signal was observed showing that cells ruptured by ultrasonication provided slightly higher signal changes than intact microbes.
ER  -

FARKA, Zdeněk; David KOVÁŘ a Petr SKLÁDAL. Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM. \textit{Sensors}. Basel, (Switzerland): MDPI AG, 2015, roč.~15, č.~1, s.~79-92. ISSN~1424-8220. Dostupné z: https://doi.org/10.3390/s150100079.

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